Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RFER_RS19695 Genome accession   NC_007908
Coordinates   4329465..4330508 (-) Length   347 a.a.
NCBI ID   WP_011466140.1    Uniprot ID   Q21RM6
Organism   Rhodoferax ferrireducens T118     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4324465..4335508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFER_RS19665 (Rfer_3872) rsmI 4324697..4325632 (-) 936 WP_041791011.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  RFER_RS19670 (Rfer_3873) - 4325632..4326018 (+) 387 WP_011466135.1 YraN family protein -
  RFER_RS19675 (Rfer_3874) - 4326055..4326648 (+) 594 WP_011466136.1 phosphoheptose isomerase -
  RFER_RS19680 (Rfer_3875) - 4326645..4327289 (+) 645 WP_011466137.1 BON domain-containing protein -
  RFER_RS19685 (Rfer_3876) - 4327333..4328247 (-) 915 WP_011466138.1 NAD(P)-dependent oxidoreductase -
  RFER_RS19690 (Rfer_3877) pilU 4328256..4329392 (-) 1137 WP_011466139.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RFER_RS19695 (Rfer_3878) pilT 4329465..4330508 (-) 1044 WP_011466140.1 type IV pilus twitching motility protein PilT Machinery gene
  RFER_RS19700 (Rfer_3879) - 4330544..4331263 (+) 720 WP_011466141.1 YggS family pyridoxal phosphate-dependent enzyme -
  RFER_RS19705 (Rfer_3880) metK 4331325..4332506 (-) 1182 WP_011466142.1 methionine adenosyltransferase -
  RFER_RS19710 (Rfer_3881) - 4332698..4333528 (+) 831 WP_011466143.1 lysophospholipid acyltransferase family protein -
  RFER_RS19715 (Rfer_3882) - 4333606..4334031 (-) 426 WP_011466144.1 Lrp/AsnC family transcriptional regulator -
  RFER_RS19720 (Rfer_3883) - 4334157..4335266 (+) 1110 WP_011466145.1 ornithine cyclodeaminase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38275.93 Da        Isoelectric Point: 6.7746

>NTDB_id=25558 RFER_RS19695 WP_011466140.1 4329465..4330508(-) (pilT) [Rhodoferax ferrireducens T118]
MDITQLLAFSVKNKASDLHLSSGLPPMIRVNGDVRRINVEALDHKQVHAMVYDIMNDSQRKVYEEFWEVDFSFEIEGLAR
FRVNAYNQQRGAGAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNFLNETEFGHILTVED
PIEFVHESKKCLVNQREVGTHTLSFAAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRV
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSSIQTGNSVGMQTLDQN
LSDLVKRNIISPSEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=25558 RFER_RS19695 WP_011466140.1 4329465..4330508(-) (pilT) [Rhodoferax ferrireducens T118]
GTGGACATCACCCAATTACTGGCCTTCAGCGTCAAGAACAAGGCGTCTGACCTGCACCTCTCCTCCGGTCTGCCCCCCAT
GATTCGCGTCAATGGCGACGTGCGGCGCATCAACGTCGAAGCGCTCGATCACAAGCAGGTGCATGCCATGGTGTATGACA
TCATGAACGACTCGCAACGCAAGGTGTATGAAGAGTTTTGGGAGGTTGACTTCTCGTTCGAGATTGAAGGGCTGGCCCGC
TTTCGGGTCAATGCCTACAACCAGCAACGCGGGGCGGGTGCGGTGTTTCGGACCATCCCGAGCAAAATCTTGACCCTGGA
GCAATTGAACGCACCGAAGATTTTTGCCGATCTGGCGCTCAAACCGCGCGGCCTGGTGCTGGTGACCGGCCCCACCGGGT
CCGGCAAGTCGACCACCCTGGCGGCCATGGTCAACTTTCTCAATGAAACCGAGTTCGGCCACATCCTGACGGTGGAAGAC
CCGATCGAGTTTGTGCATGAGTCCAAAAAATGTCTGGTCAACCAGCGCGAGGTTGGCACCCACACGCTCAGTTTTGCCGC
CGCCCTGCGCTCGGCCCTGCGCGAAGACCCGGACGCCATTCTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACCGCCGCCGAAACCGGCCACCTGGTGTTTGGCACCTTGCATACCTCCAGCGCTGCCAAGACCATTGACCGCGTC
ATCGATGTCTTTCCGGCCGAAGAAAAAGAGATGGTGCGCGCCATGTTGTCCGAATCCCTGCAGGCGGTGATCTCGCAGAC
CCTGTGCAAAACCAAGGACGGCCAGGGCCGGGTTGCGGCGCACGAGATCATGCTCGGCACCAGCGCCATCCGCAATTTGA
TCCGTGAGGCCAAGGTGGCGCAGATGTATTCGAGCATTCAGACCGGCAACAGTGTGGGCATGCAGACACTGGATCAGAAC
CTGAGTGATCTGGTCAAACGCAACATCATCAGTCCGTCGGAAGCGCGCTCCAAAGCCAAGATTCCGGAGAATTTCCCCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q21RM6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.391

99.424

0.769
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

73.156

97.695

0.715
  pilT Legionella pneumophila strain ERS1305867

73.156

97.695

0.715
  pilT Neisseria meningitidis 8013

67.246

99.424

0.669
  pilT Neisseria gonorrhoeae MS11

66.957

99.424

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.569

98.271

0.654
  pilT Vibrio cholerae strain A1552

66.569

98.271

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

40.173

99.712

0.401
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment