Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RFER_RS19690 Genome accession   NC_007908
Coordinates   4328256..4329392 (-) Length   378 a.a.
NCBI ID   WP_011466139.1    Uniprot ID   Q21RM7
Organism   Rhodoferax ferrireducens T118     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4323256..4334392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFER_RS19655 (Rfer_3870) - 4323817..4324140 (-) 324 WP_011466132.1 EthD family reductase -
  RFER_RS19660 (Rfer_3871) - 4324233..4324586 (-) 354 WP_011466133.1 MerR family transcriptional regulator -
  RFER_RS19665 (Rfer_3872) rsmI 4324697..4325632 (-) 936 WP_041791011.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  RFER_RS19670 (Rfer_3873) - 4325632..4326018 (+) 387 WP_011466135.1 YraN family protein -
  RFER_RS19675 (Rfer_3874) - 4326055..4326648 (+) 594 WP_011466136.1 phosphoheptose isomerase -
  RFER_RS19680 (Rfer_3875) - 4326645..4327289 (+) 645 WP_011466137.1 BON domain-containing protein -
  RFER_RS19685 (Rfer_3876) - 4327333..4328247 (-) 915 WP_011466138.1 NAD(P)-dependent oxidoreductase -
  RFER_RS19690 (Rfer_3877) pilU 4328256..4329392 (-) 1137 WP_011466139.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RFER_RS19695 (Rfer_3878) pilT 4329465..4330508 (-) 1044 WP_011466140.1 type IV pilus twitching motility protein PilT Machinery gene
  RFER_RS19700 (Rfer_3879) - 4330544..4331263 (+) 720 WP_011466141.1 YggS family pyridoxal phosphate-dependent enzyme -
  RFER_RS19705 (Rfer_3880) metK 4331325..4332506 (-) 1182 WP_011466142.1 methionine adenosyltransferase -
  RFER_RS19710 (Rfer_3881) - 4332698..4333528 (+) 831 WP_011466143.1 lysophospholipid acyltransferase family protein -
  RFER_RS19715 (Rfer_3882) - 4333606..4334031 (-) 426 WP_011466144.1 Lrp/AsnC family transcriptional regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41835.16 Da        Isoelectric Point: 6.9076

>NTDB_id=25557 RFER_RS19690 WP_011466139.1 4328256..4329392(-) (pilU) [Rhodoferax ferrireducens T118]
MERDQATKFINDLLKLMISRNGSDLFLTADFPPAIKVDGKVTKVSPQALTAAHTLSLARSIMSDKQVAEFERTKECNFAI
APPGVGRFRVSAFIQQGRVGLVLRVIPLSVPTIDGLGVPPILKEVASAKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHQHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKALVSQRLIPKQDGRGRYAVVEIMLNTPLISDMIFKGEVSEIKEIMKKSRNLG
MQTFDQALYDAYEANLITYEDALRNADSLNDLRLQIKLNSHRGKAQDLNAGTENFSIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=25557 RFER_RS19690 WP_011466139.1 4328256..4329392(-) (pilU) [Rhodoferax ferrireducens T118]
ATGGAACGCGATCAAGCCACCAAATTCATCAACGATCTGCTCAAGCTGATGATCAGCCGCAACGGCAGCGATTTGTTCCT
CACGGCCGATTTTCCGCCCGCCATCAAGGTGGACGGCAAGGTCACCAAGGTCTCGCCCCAAGCGCTCACCGCGGCGCATA
CCCTGTCGTTGGCGCGCTCCATCATGAGCGACAAGCAGGTGGCCGAGTTTGAACGCACCAAAGAATGCAACTTTGCCATC
GCACCGCCCGGTGTTGGCCGTTTTCGCGTCAGCGCCTTTATCCAGCAGGGCCGGGTTGGCTTGGTGCTGCGGGTCATACC
GCTGAGCGTGCCCACTATTGATGGTTTGGGTGTGCCCCCGATACTCAAGGAAGTGGCCTCTGCCAAGCGCGGTTTGTGCA
TTTTGGTGGGAGCTACCGGCTCCGGCAAATCGACCACCCTGGCCGCGATGGTTGACTGGCGCAACGAAAACTCGTTTGGT
CACATCATCACGGTTGAAGACCCGATTGAGTTTGTGCATCAGCACAAAAACTGTGTGGTGACCCAGCGCGAGGTCGGCAT
CGACACCGACAGTTGGGAAGCGGCGCTCAAAAATACCCTGCGCCAGGCGCCCGATGTCATTCTGATGGGCGAAATTCGCG
ACCGCGAAACCATGGAACATGCGGTCGCGTTTGCCGAGACCGGGCATTTGTGTCTCGCCACCTTGCACGCCAACAGCGCC
AACCAAGCGCTGGACCGGATCATCAATTTCTTCCCTGAAGAGCGCCGCGCGCAGTTGCTGATGGACTTGTCGCTCAACCT
GAAGGCGCTGGTGTCGCAGCGGCTGATCCCGAAACAGGATGGTCGCGGGCGCTATGCGGTGGTCGAGATCATGCTCAACA
CGCCGCTTATCTCCGACATGATCTTCAAGGGCGAAGTCTCCGAGATCAAGGAGATCATGAAGAAAAGCCGCAACCTGGGC
ATGCAGACTTTCGACCAGGCCTTGTACGACGCCTACGAAGCCAACCTGATCACCTACGAGGACGCCCTGCGCAACGCCGA
TTCACTCAATGACCTGCGCCTGCAGATCAAGCTCAACAGCCATCGCGGCAAAGCCCAGGACCTGAACGCCGGTACCGAAA
ATTTCTCCATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q21RM7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.793

92.063

0.587
  pilU Acinetobacter baylyi ADP1

58.496

94.974

0.556
  pilU Vibrio cholerae strain A1552

54.31

92.063

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Legionella pneumophila strain ERS1305867

43.544

88.095

0.384
  pilT Legionella pneumophila strain Lp02

43.544

88.095

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baumannii D1279779

45.141

84.392

0.381
  pilT Acinetobacter baumannii strain A118

45.141

84.392

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.479

83.862

0.373
  pilT Vibrio cholerae strain A1552

44.479

83.862

0.373
  pilT Acinetobacter baylyi ADP1

43.574

84.392

0.368

Multiple sequence alignment