Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   CPZ87_RS01210 Genome accession   NZ_CP024645
Coordinates   268234..268656 (+) Length   140 a.a.
NCBI ID   WP_085748872.1    Uniprot ID   A0A1W6L2Y2
Organism   Piscinibacter gummiphilus strain NBRC 109400     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 263234..273656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPZ87_RS01185 (CPZ87_01185) - 263313..264065 (+) 753 WP_085748867.1 TerC family protein -
  CPZ87_RS01190 (CPZ87_01190) - 264124..265377 (+) 1254 WP_085748868.1 serine/threonine-protein kinase -
  CPZ87_RS01195 (CPZ87_01195) - 265439..266221 (+) 783 WP_085748869.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  CPZ87_RS01200 (CPZ87_01200) - 266304..266987 (+) 684 WP_085748870.1 FHA domain-containing protein -
  CPZ87_RS01205 (CPZ87_01205) - 267247..268023 (+) 777 WP_085748871.1 MBL fold metallo-hydrolase -
  CPZ87_RS01210 (CPZ87_01210) pilA2 268234..268656 (+) 423 WP_085748872.1 pilin Machinery gene
  CPZ87_RS30520 (CPZ87_01215) pilA 268719..269144 (+) 426 WP_085748873.1 pilin Machinery gene
  CPZ87_RS01220 (CPZ87_01220) - 269141..270997 (+) 1857 WP_085748874.1 Wzy polymerase domain-containing protein -
  CPZ87_RS01225 (CPZ87_01225) tfpZ 271095..271886 (+) 792 WP_085748875.1 TfpX/TfpZ family type IV pilin accessory protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14410.29 Da        Isoelectric Point: 7.7771

>NTDB_id=254680 CPZ87_RS01210 WP_085748872.1 268234..268656(+) (pilA2) [Piscinibacter gummiphilus strain NBRC 109400]
MKRSIQKGFTLIELMIVVAIIGILAAVALPAYQDYVKRSRISEGLALASGAKTALAEYVASNNEWPASGSAAGIEDGTAI
NGNSVNSVTVGTSNGQITIEYASKAGGNNIVIQAATTAGGVTWDCRGGSVEGKYRPSECR

Nucleotide


Download         Length: 423 bp        

>NTDB_id=254680 CPZ87_RS01210 WP_085748872.1 268234..268656(+) (pilA2) [Piscinibacter gummiphilus strain NBRC 109400]
ATGAAGCGTTCCATCCAAAAGGGTTTCACCCTCATCGAACTGATGATCGTCGTCGCGATCATCGGCATCCTGGCCGCCGT
CGCTCTGCCGGCCTACCAGGACTACGTGAAGCGTTCGCGCATCTCTGAAGGCCTGGCGCTGGCTTCGGGTGCGAAGACCG
CCCTGGCCGAGTACGTCGCCTCGAACAACGAATGGCCGGCCAGCGGCTCCGCGGCTGGCATTGAAGATGGCACCGCCATC
AATGGCAACTCGGTGAACTCGGTCACGGTCGGTACGTCGAACGGTCAAATCACCATCGAGTACGCCAGCAAGGCCGGTGG
CAACAACATCGTCATTCAAGCTGCGACGACCGCCGGTGGCGTGACCTGGGACTGCCGCGGCGGCTCGGTCGAAGGCAAGT
ACCGCCCCAGCGAGTGCCGCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)

Pilin (bacterial filament) (Pilin)

(38-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W6L2Y2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

54.412

97.143

0.529
  pilA2 Legionella pneumophila strain ERS1305867

53.676

97.143

0.521
  comP Acinetobacter baylyi ADP1

46.528

100

0.479
  pilA Ralstonia pseudosolanacearum GMI1000

40

100

0.471
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.759

100

0.464
  pilE Neisseria gonorrhoeae MS11

39.623

100

0.45
  pilE Neisseria gonorrhoeae strain FA1090

39.873

100

0.45
  pilA Vibrio cholerae C6706

43.056

100

0.443
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.056

100

0.443
  pilA Vibrio cholerae strain A1552

43.056

100

0.443
  pilA/pilA1 Eikenella corrodens VA1

37.908

100

0.414
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.86

100

0.407
  pilA Pseudomonas aeruginosa PAK

37.748

100

0.407
  pilA Vibrio campbellii strain DS40M4

35.484

100

0.393
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.298

100

0.386
  pilA Haemophilus influenzae 86-028NP

40.769

92.857

0.379

Multiple sequence alignment