Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DIW83_RS14470 Genome accession   NZ_CP029351
Coordinates   2994031..2995068 (+) Length   345 a.a.
NCBI ID   WP_006581387.1    Uniprot ID   A0AA36KDN2
Organism   Acinetobacter nosocomialis strain NCTC 8102     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2989031..3000068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DIW83_RS14460 (DIW83_14460) - 2992262..2993104 (-) 843 WP_006581385.1 undecaprenyl-diphosphate phosphatase -
  DIW83_RS14465 (DIW83_14465) - 2993212..2993904 (-) 693 WP_016804433.1 YggS family pyridoxal phosphate-dependent enzyme -
  DIW83_RS14470 (DIW83_14470) pilT 2994031..2995068 (+) 1038 WP_006581387.1 type IV pilus twitching motility protein PilT Machinery gene
  DIW83_RS14475 (DIW83_14475) pilU 2995096..2996214 (+) 1119 WP_002052019.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DIW83_RS14480 (DIW83_14480) fur 2996284..2996721 (-) 438 WP_002052029.1 ferric iron uptake transcriptional regulator -
  DIW83_RS14485 (DIW83_14485) - 2996833..2997231 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  DIW83_RS14490 (DIW83_14490) - 2997287..2997610 (-) 324 WP_002052062.1 RnfH family protein -
  DIW83_RS14495 (DIW83_14495) - 2997610..2998677 (-) 1068 WP_004710227.1 hypothetical protein -
  DIW83_RS14505 (DIW83_14505) - 2998830..2999285 (-) 456 WP_000782976.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38328.92 Da        Isoelectric Point: 6.7271

>NTDB_id=252933 DIW83_RS14470 WP_006581387.1 2994031..2995068(+) (pilT) [Acinetobacter nosocomialis strain NCTC 8102]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=252933 DIW83_RS14470 WP_006581387.1 2994031..2995068(+) (pilT) [Acinetobacter nosocomialis strain NCTC 8102]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCATCCGATTTGCACTTATCAGCTGGTATGCCACCAAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATTAACTTGCCAGCCTTAGAACATAAAGATGTCCATCGCCTCGTTTATGACA
TCATGAATGATAAACAGCGTCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCGAATGTTGCGCGT
TTCCGTGTGAACGCATTTAACCAAAATCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACGATGGA
AGATCTAGGTCTAGGGCAAATCTTTAAAGATATTTGCGACTATCCTCGCGGGATTGTACTGGTGACAGGACCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCAATGCTCGATTATATTAATGAAAACCGCTATGACCATATTTTAACGGTGGAAGAC
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTGATTAACCAGCGTGAAGTGCATCGTGATACACATGGCTTTAATGA
AGCCCTTCGTTCAGCACTACGTGAAGACCCAGATATTATTTTAGTCGGTGAGATGCGTGACCTTGAAACGATTCGATTAG
CACTTACTGCTGCCGAAACAGGTCACTTGGTATTTGGTACGCTTCACACCACATCGGCTGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCTTTACAAGCCGTTATTTCACAAAC
TCTGCTTAAAAAGAATGGTGGTGGTCGTGTTGCGGCACATGAAATTATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTATTCAGCTATTCAGACTGGTGCTAACCATGGTATGACAACCCTCGACCAAAGCCTG
AAAGGTTTGGTGTCACGTGGTGTAATTAGTCCACAAACAGCCCGTACAGCGGCGAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0AA36KDN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

100

100

1
  pilT Acinetobacter baumannii D1279779

99.71

100

0.997
  pilT Acinetobacter baumannii strain A118

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment