Detailed information NTG0252934

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: DIW83_RS14475
• Genome accession: NZ_CP029351
• Coordinates: 2995096..2996214 (+)
• Length: 372 a.a.
• NCBI ID: WP_002052019.1
• Uniprot ID: A0AA36KFD7
• Organism: Acinetobacter nosocomialis strain NCTC 8102
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2990096..3001214

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DIW83_RS14460 (DIW83_14460)	-	2992262..2993104 (-)	843	WP_006581385.1	undecaprenyl-diphosphate phosphatase	-
DIW83_RS14465 (DIW83_14465)	-	2993212..2993904 (-)	693	WP_016804433.1	YggS family pyridoxal phosphate-dependent enzyme	-
DIW83_RS14470 (DIW83_14470)	pilT	2994031..2995068 (+)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
DIW83_RS14475 (DIW83_14475)	pilU	2995096..2996214 (+)	1119	WP_002052019.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
DIW83_RS14480 (DIW83_14480)	fur	2996284..2996721 (-)	438	WP_002052029.1	ferric iron uptake transcriptional regulator	-
DIW83_RS14485 (DIW83_14485)	-	2996833..2997231 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
DIW83_RS14490 (DIW83_14490)	-	2997287..2997610 (-)	324	WP_002052062.1	RnfH family protein	-
DIW83_RS14495 (DIW83_14495)	-	2997610..2998677 (-)	1068	WP_004710227.1	hypothetical protein	-
DIW83_RS14505 (DIW83_14505)	-	2998830..2999285 (-)	456	WP_000782976.1	bacteriohemerythrin	-
DIW83_RS14510 (DIW83_14510)	-	2999538..3000386 (-)	849	WP_016804434.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41980.18 Da
• Isoelectric Point: 6.6944

>NTDB_id=252934 DIW83_RS14475 WP_002052019.1 2995096..2996214(+) (pilU) [Acinetobacter nosocomialis strain NCTC 8102]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLANEGPDQITDEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=252934 DIW83_RS14475 WP_002052019.1 2995096..2996214(+) (pilU) [Acinetobacter nosocomialis strain NCTC 8102]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTAATTGGTCAACTCTTACATTCCA
TCATGAGTGAAAAACAACGTAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGCGATAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGTGACATGCCGGGTATGGTACTACGTCGAATTGAAACGAAAATTCCTTCAAT
TGATGACTTACAGTTGCCGACTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAG
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAACGTGAAGTCGGTATTGATACGGACTCTTTCGA
AATTGCTTTAAAAAATACATTACGACAAGCACCAGATGTGATCTTAATTGGTGAAATCCGCTCTCGTGAAGTCATGGACT
ACGCAATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACAATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGACTTGTCTCTTAACTTAAAAGCCATGATTGCACA
GCAGCTTATTCCAACACCAGATGGTAATTCACGCCGTGCAGCGATTGAGATCTTAATTAACTCACCATTAATTTCAGACT
ATATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTGATGAAACGCTCACGTGAGCTTGGTATGCAGACCTTTGACCAA
GCTTTATTCGACTTATATAAAGCGGGCCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTAACGATTAAGCTTGCAAATGAAGGTCCTGATCAAATTACAGACGAAAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0AA36KFD7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.254	95.43	0.403