Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CK910_RS08745 Genome accession   NZ_CP023818
Coordinates   1842542..1843576 (-) Length   344 a.a.
NCBI ID   WP_042063177.1    Uniprot ID   A0A6S5C6A2
Organism   Aeromonas sp. CA23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1837542..1848576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK910_RS08725 (CK910_08725) - 1838346..1839056 (-) 711 WP_098982291.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  CK910_RS08730 (CK910_08730) srmB 1839185..1840408 (+) 1224 WP_098982293.1 ATP-dependent RNA helicase SrmB -
  CK910_RS08735 (CK910_08735) yaaA 1840599..1841372 (-) 774 WP_043142400.1 peroxide stress protein YaaA -
  CK910_RS08740 (CK910_08740) pilU 1841404..1842513 (-) 1110 WP_043142397.1 type IVa pilus ATPase TapU Machinery gene
  CK910_RS08745 (CK910_08745) pilT 1842542..1843576 (-) 1035 WP_042063177.1 type IVa pilus ATPase TapT Machinery gene
  CK910_RS08750 (CK910_08750) - 1843616..1844317 (+) 702 WP_098982296.1 YggS family pyridoxal phosphate-dependent enzyme -
  CK910_RS08755 (CK910_08755) proC 1844408..1845232 (+) 825 WP_098982299.1 pyrroline-5-carboxylate reductase -
  CK910_RS08760 (CK910_08760) - 1845261..1845812 (+) 552 WP_043142388.1 YggT family protein -
  CK910_RS08765 (CK910_08765) yggU 1845812..1846111 (+) 300 WP_043554521.1 DUF167 family protein YggU -
  CK910_RS08770 (CK910_08770) - 1846130..1846549 (+) 420 WP_098982302.1 DUF4426 domain-containing protein -
  CK910_RS08775 (CK910_08775) - 1846619..1846918 (-) 300 WP_098982304.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38291.00 Da        Isoelectric Point: 6.7439

>NTDB_id=250336 CK910_RS08745 WP_042063177.1 1842542..1843576(-) (pilT) [Aeromonas sp. CA23]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKISEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSV

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=250336 CK910_RS08745 WP_042063177.1 1842542..1843576(-) (pilT) [Aeromonas sp. CA23]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCCGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACAGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCACCAGCGCAAGGAGCTGGAGGAAAACTTCGAGGTCGATTTCTCGTTCGAGGTGCCCAACCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGTTCGGGCGCCGTGTTTCGTACCATCCCCAGCACAGTGCTGAGTTTGGA
AGATCTCGATGCCCCCGAGATCTTTCGCAAGATCTCCGAATACCCCCGCGGCCTGGTGCTGGTGACAGGGCCGACCGGCT
CGGGCAAATCGACCACCTTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCACCACCACATACTCACCATCGAAGAC
CCCATCGAATTCGTGCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTACACAGGGATACCAAGAGCTTCAGTAA
CGCCCTGCGCTCGGCACTGCGGGAAGATCCTGACATCATACTGGTAGGCGAGATGCGTGACCTCGAGACCATACGCCTGG
CCATGACGGCGGCCGAGACGGGCCACCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATAGACCGTATC
ATCGACGTCTTCCCCGGCGCGGAAAAAGACATGGTGCGATCCATGCTCTCCGAATCGCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGGATCGGCGGTGGCCGGGTGGCGGCGCACGAGATCATGATGGGCATACCCGCGGTGCGCAACCTCATCC
GGGAAGACAAGATCGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTCAGCCGTGGCGTGGTGGCCTCCCTGGATGCCAAGGCCAAGGCGGTCGACCCCAATAGCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6S5C6A2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.843

99.709

0.776
  pilT Acinetobacter baylyi ADP1

77.843

99.709

0.776
  pilT Acinetobacter baumannii D1279779

77.551

99.709

0.773
  pilT Acinetobacter baumannii strain A118

77.551

99.709

0.773
  pilT Pseudomonas stutzeri DSM 10701

76.385

99.709

0.762
  pilT Pseudomonas aeruginosa PAK

75.802

99.709

0.756
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.353

98.837

0.715
  pilT Legionella pneumophila strain ERS1305867

72.353

98.837

0.715
  pilT Neisseria meningitidis 8013

69.565

100

0.698
  pilT Neisseria gonorrhoeae MS11

69.275

100

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.462

99.419

0.512
  pilU Vibrio cholerae strain A1552

42.609

100

0.427
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404

Multiple sequence alignment