Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CK910_RS08740 Genome accession   NZ_CP023818
Coordinates   1841404..1842513 (-) Length   369 a.a.
NCBI ID   WP_043142397.1    Uniprot ID   A0AAW7HZS2
Organism   Aeromonas sp. CA23     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1836404..1847513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK910_RS08720 (CK910_08720) brnQ 1836848..1838155 (-) 1308 WP_098985046.1 branched-chain amino acid transport system II carrier protein -
  CK910_RS08725 (CK910_08725) - 1838346..1839056 (-) 711 WP_098982291.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  CK910_RS08730 (CK910_08730) srmB 1839185..1840408 (+) 1224 WP_098982293.1 ATP-dependent RNA helicase SrmB -
  CK910_RS08735 (CK910_08735) yaaA 1840599..1841372 (-) 774 WP_043142400.1 peroxide stress protein YaaA -
  CK910_RS08740 (CK910_08740) pilU 1841404..1842513 (-) 1110 WP_043142397.1 type IVa pilus ATPase TapU Machinery gene
  CK910_RS08745 (CK910_08745) pilT 1842542..1843576 (-) 1035 WP_042063177.1 type IVa pilus ATPase TapT Machinery gene
  CK910_RS08750 (CK910_08750) - 1843616..1844317 (+) 702 WP_098982296.1 YggS family pyridoxal phosphate-dependent enzyme -
  CK910_RS08755 (CK910_08755) proC 1844408..1845232 (+) 825 WP_098982299.1 pyrroline-5-carboxylate reductase -
  CK910_RS08760 (CK910_08760) - 1845261..1845812 (+) 552 WP_043142388.1 YggT family protein -
  CK910_RS08765 (CK910_08765) yggU 1845812..1846111 (+) 300 WP_043554521.1 DUF167 family protein YggU -
  CK910_RS08770 (CK910_08770) - 1846130..1846549 (+) 420 WP_098982302.1 DUF4426 domain-containing protein -
  CK910_RS08775 (CK910_08775) - 1846619..1846918 (-) 300 WP_098982304.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41097.15 Da        Isoelectric Point: 6.4431

>NTDB_id=250335 CK910_RS08740 WP_043142397.1 1841404..1842513(-) (pilU) [Aeromonas sp. CA23]
MSLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLGGEPLDKKSALTLVKETLSQDHFERYIRTKEANYAIHREDLGR
FRVSAFWQQDLPGMVLRRIETRIPTFDELTLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSVDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=250335 CK910_RS08740 WP_043142397.1 1841404..1842513(-) (pilU) [Aeromonas sp. CA23]
ATGAGTCTGGATGCCCTGTTGACCGAACTGGTCGCACGCAAAGGTTCGGATCTGTTCATCACGGTCGGCACGCCCCCCAC
CCTCAAGGTGAACGGCCATCTGCAGTCCCTGGGGGGAGAGCCGCTCGACAAGAAGAGTGCCCTGACCCTGGTCAAGGAGA
CATTAAGCCAGGATCACTTCGAGCGTTACATCCGCACCAAGGAAGCCAACTACGCCATCCACCGCGAGGATCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGATCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGGATCCCGACCTTCGA
CGAGCTGACCCTGCCCCCCATCTTGCAGGAGGTGGCCATGGCCAAGCGCGGGCTTGTGCTGTTCGTCGGCGCCACTGGTG
CGGGCAAGTCCACCACCCAGGCCGCCATGATAGGGTTTCGCAACCAGCACGCCGATGGCCATATCCTGACGGTGGAAGAC
CCGGTGGAATTCGTGCACCAGCACGGCCGCAGCCTGATCACCCAGCGGGAGGTGGGGATAGACACGGAGTCGTTCGACGT
GGCGCTGAAGAGCTCGTTGCGCCAGGCACCGGACGTGATCCTCATCGGCGAGATCCGCAGCCAGGAGACCATGGAGTTTG
CGCTGCAATTTGCCGAGACGGGTCACCTCTGTCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCACCTGGTACCGCAGGAGAAACACCGCCAGTTCCTGTTTGACCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
ACTGCTGCCGAGCGTGGACGGTCGCCGCCGTTTTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCTACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCCGGCCGTGAACAGCTGGGTGCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.054

99.729

0.599
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

58.074

95.664

0.556
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.9

89.702

0.385
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363

Multiple sequence alignment