Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CK911_RS19750 Genome accession   NZ_CP023817
Coordinates   4360736..4361770 (+) Length   344 a.a.
NCBI ID   WP_042024218.1    Uniprot ID   -
Organism   Aeromonas sp. CU5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4355736..4366770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK911_RS19720 (CK911_19720) - 4356432..4357628 (+) 1197 WP_098972105.1 NupC/NupG family nucleoside CNT transporter -
  CK911_RS19725 (CK911_19725) - 4357700..4358119 (-) 420 WP_098972107.1 DUF4426 domain-containing protein -
  CK911_RS19730 (CK911_19730) yggU 4358163..4358465 (-) 303 WP_098972109.1 DUF167 family protein YggU -
  CK911_RS19735 (CK911_19735) - 4358465..4359016 (-) 552 WP_068977360.1 YggT family protein -
  CK911_RS19740 (CK911_19740) proC 4359028..4359852 (-) 825 WP_098972111.1 pyrroline-5-carboxylate reductase -
  CK911_RS19745 (CK911_19745) - 4359995..4360696 (-) 702 WP_098972113.1 YggS family pyridoxal phosphate-dependent enzyme -
  CK911_RS19750 (CK911_19750) pilT 4360736..4361770 (+) 1035 WP_042024218.1 type IVa pilus ATPase TapT Machinery gene
  CK911_RS19755 (CK911_19755) pilU 4361799..4362908 (+) 1110 WP_098972116.1 type IVa pilus ATPase TapU Machinery gene
  CK911_RS19760 (CK911_19760) yaaA 4362956..4363729 (+) 774 WP_098972118.1 peroxide stress protein YaaA -
  CK911_RS19765 (CK911_19765) - 4363926..4364351 (+) 426 WP_042024223.1 hypothetical protein -
  CK911_RS19770 (CK911_19770) - 4364715..4365206 (-) 492 WP_098972120.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  CK911_RS19775 (CK911_19775) arfB 4365237..4365653 (-) 417 WP_098972122.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  CK911_RS19780 (CK911_19780) glnK 4365855..4366193 (+) 339 WP_005341406.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38349.08 Da        Isoelectric Point: 6.7440

>NTDB_id=250316 CK911_RS19750 WP_042024218.1 4360736..4361770(+) (pilT) [Aeromonas sp. CU5]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHSLIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQSRGSGAVFRTIPSTVLSLEDLDAPEIFRKISEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKVKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=250316 CK911_RS19750 WP_042024218.1 4360736..4361770(+) (pilT) [Aeromonas sp. CU5]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGTGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTGCGCAAGATCAATTTGCCCGCCCTCGAACATCGAGAAGTACATAGCCTCATTTATGACA
TCATGAACGATCACCAGCGCAAGGAGCTGGAAGAGAACTTCGAAGTCGATTTCTCGTTTGAGGTGCCGAATTTGGCCCGT
TTCCGGGTTAACGCCTTTCAGCAATCCCGCGGATCAGGCGCAGTATTTCGGACCATCCCCAGCACGGTACTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTTCGCAAAATTTCGGAATTTCCCCGAGGTCTGGTGCTGGTGACCGGCCCGACCGGTT
CTGGTAAGTCGACCACCTTGGCGGCCATGGTCAACTACATCAATGAGAACTTCCACCACCACATTCTCACCATCGAAGAC
CCCATCGAATTCGTTCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTGCACCGGGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCTCTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGTGACCTCGAGACCATTCGCCTCG
CCATGACTGCCGCCGAAACCGGCCATCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCTAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGTGCGGAGAAAGACATGGTGCGTTCCATGCTCTCCGAATCCCTGCGGGCGGTTATCTCCCAGAC
CCTGCTCAAGCGCATTGGCGGTGGTCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCCGCGGTGCGTAACCTCATTC
GGGAAGACAAGATCGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAAAGCCTG
AAACAGCTGGTTAGCCGCGGTGTGGTGGCATCCCTCGATGCCAAGGTCAAGGCGGTTGATCCCAACAGTATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Acinetobacter baylyi ADP1

77.259

99.709

0.77
  pilT Pseudomonas stutzeri DSM 10701

75.872

100

0.759
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.371

97.965

0.738
  pilT Vibrio cholerae strain A1552

75.371

97.965

0.738
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.565

100

0.698
  pilT Neisseria gonorrhoeae MS11

69.275

100

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.462

99.419

0.512
  pilU Vibrio cholerae strain A1552

42.319

100

0.424
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404

Multiple sequence alignment