Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CK911_RS19755 Genome accession   NZ_CP023817
Coordinates   4361799..4362908 (+) Length   369 a.a.
NCBI ID   WP_098972116.1    Uniprot ID   A0A291TNT0
Organism   Aeromonas sp. CU5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4356799..4367908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK911_RS19725 (CK911_19725) - 4357700..4358119 (-) 420 WP_098972107.1 DUF4426 domain-containing protein -
  CK911_RS19730 (CK911_19730) yggU 4358163..4358465 (-) 303 WP_098972109.1 DUF167 family protein YggU -
  CK911_RS19735 (CK911_19735) - 4358465..4359016 (-) 552 WP_068977360.1 YggT family protein -
  CK911_RS19740 (CK911_19740) proC 4359028..4359852 (-) 825 WP_098972111.1 pyrroline-5-carboxylate reductase -
  CK911_RS19745 (CK911_19745) - 4359995..4360696 (-) 702 WP_098972113.1 YggS family pyridoxal phosphate-dependent enzyme -
  CK911_RS19750 (CK911_19750) pilT 4360736..4361770 (+) 1035 WP_042024218.1 type IVa pilus ATPase TapT Machinery gene
  CK911_RS19755 (CK911_19755) pilU 4361799..4362908 (+) 1110 WP_098972116.1 type IVa pilus ATPase TapU Machinery gene
  CK911_RS19760 (CK911_19760) yaaA 4362956..4363729 (+) 774 WP_098972118.1 peroxide stress protein YaaA -
  CK911_RS19765 (CK911_19765) - 4363926..4364351 (+) 426 WP_042024223.1 hypothetical protein -
  CK911_RS19770 (CK911_19770) - 4364715..4365206 (-) 492 WP_098972120.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  CK911_RS19775 (CK911_19775) arfB 4365237..4365653 (-) 417 WP_098972122.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  CK911_RS19780 (CK911_19780) glnK 4365855..4366193 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  CK911_RS19785 (CK911_19785) - 4366451..4367458 (+) 1008 WP_098972124.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41079.07 Da        Isoelectric Point: 6.5036

>NTDB_id=250317 CK911_RS19755 WP_098972116.1 4361799..4362908(+) (pilU) [Aeromonas sp. CU5]
MNLDDLLSQLVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQEQPGMVVRRIETRIPTFEDLQLPNILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNKHADGHILTVED
PVEFVHQHGRCLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRFAAFEVLLNTPLITDIIRKGEIHRLKEVMTKSSELGMQTFDQA
LFKLFCAGQIGYSEALAHADSANDLRLQIKLSGQDTLGTGMLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=250317 CK911_RS19755 WP_098972116.1 4361799..4362908(+) (pilU) [Aeromonas sp. CU5]
ATGAATCTGGATGATCTGCTGAGCCAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGTTCACCGCCCAC
CCTCAAGGTGAACGGTCATCTTGTATCGCTGGGGGGGGAGGCGCTAGACAAGAAGGGGGCACTGACGCTGGTCAGGGATA
CCTTGAGCAGCGATCACTTCGAGCGCTATATCCGTACCAAGGAGGCCAACTACGCGATTTATCGCGAGGCCCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCAGCCCGGCATGGTGGTGCGCCGCATCGAAACCCGCATCCCCACCTTTGA
AGACCTGCAACTGCCCAATATCTTGCAAGAGGTCGCGATGGCCAAGCGGGGATTGGTGCTGTTTGTCGGGGCTACCGGGG
CAGGTAAATCCACCACCCAGGCGGCAATGATTGGCTATCGCAACAAGCATGCCGATGGCCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGCCGTTGTTTGGTGACCCAGCGAGAGGTGGGGATCGATACCGAGTCATTCGATGT
GGCGCTGAAAAGCTCGCTGCGTCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACGGGCCACCTCTGTCTCGCCACCTTGCATGCCAACAACGCCAATCAGGCGCTGGATCGCATT
CTCCATCTGGTGCCGCAAGACAAACACCGTCAGTTCCTGTTCGATCTCTCCTTCAATCTCAAGGCCATCGTTGCCCAGCA
GCTGGTGCCCAGCATGGATGGCAAGCGGCGTTTTGCGGCCTTCGAGGTGCTGCTCAATACCCCGCTGATCACCGATATCA
TCCGCAAGGGTGAGATACACAGGCTCAAGGAGGTGATGACCAAATCCTCTGAGTTGGGCATGCAGACTTTCGATCAGGCG
TTGTTCAAGCTGTTCTGTGCTGGCCAGATTGGCTACAGTGAGGCCCTCGCCCATGCCGATTCGGCCAATGATTTGCGCTT
GCAAATCAAACTCTCCGGTCAGGATACTCTCGGTACCGGCATGCTGGATAATGTAACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A291TNT0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

60.335

97.019

0.585
  pilU Acinetobacter baylyi ADP1

58.074

95.664

0.556
  pilT Legionella pneumophila strain Lp02

41.108

92.954

0.382
  pilT Legionella pneumophila strain ERS1305867

41.108

92.954

0.382
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.233

91.87

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36

Multiple sequence alignment