Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CPC19_RS01150 Genome accession   NZ_CP023664
Coordinates   217637..218674 (+) Length   345 a.a.
NCBI ID   WP_096910734.1    Uniprot ID   -
Organism   Cycloclasticus sp. PY97N     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 212637..223674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPC19_RS01130 (CPC19_01130) - 213243..215174 (-) 1932 WP_016390295.1 dynamin family protein -
  CPC19_RS01135 (CPC19_01135) - 215409..215963 (-) 555 WP_016390294.1 YggT family protein -
  CPC19_RS01140 (CPC19_01140) proC 215963..216790 (-) 828 WP_016390293.1 pyrroline-5-carboxylate reductase -
  CPC19_RS01145 (CPC19_01145) - 216824..217513 (-) 690 WP_016390292.1 YggS family pyridoxal phosphate-dependent enzyme -
  CPC19_RS01150 (CPC19_01150) pilT 217637..218674 (+) 1038 WP_096910734.1 type IV pilus twitching motility protein PilT Machinery gene
  CPC19_RS01155 (CPC19_01155) pilU 218699..219826 (+) 1128 WP_015004886.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CPC19_RS01160 (CPC19_01160) - 219994..220422 (-) 429 WP_016390291.1 type IV pilin protein -
  CPC19_RS01165 (CPC19_01165) - 220598..221122 (+) 525 WP_016390290.1 GspH/FimT family pseudopilin -
  CPC19_RS01170 (CPC19_01170) - 221116..221772 (+) 657 WP_016390289.1 PilW family protein -
  CPC19_RS01175 (CPC19_01175) - 221778..223091 (+) 1314 WP_016390288.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38585.37 Da        Isoelectric Point: 6.2885

>NTDB_id=248980 CPC19_RS01150 WP_096910734.1 217637..218674(+) (pilT) [Cycloclasticus sp. PY97N]
MDISDLLAFSTKNNASDLHLSAGLPPMIRVDGDIRRINMPELEHKEVHAMIYDIMNDKQRRDFEEFLEVDFSFELPGTAR
FRVNAFNQDRGAAAVFRTIPVDILTLEDLNCPQSFKEIINVPRGIVLVTGPTGSGKSTTLAALMDYKNDNVYEHILTIED
PIEFVHKSKKCLINQREVHKHTHGFSEALRVALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDLI
IDVFPEGEKGMVRSMLSESLRAVIAQTLMKKIGGGRIAAWEIMTGTAAIRNLIREDKIAQMYSTIQTSRKDGMQTLDQHL
TELVQAGVITRQDARMKAADKKAFS

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=248980 CPC19_RS01150 WP_096910734.1 217637..218674(+) (pilT) [Cycloclasticus sp. PY97N]
ATGGATATTTCAGATTTATTAGCCTTTAGCACCAAAAATAACGCCTCTGACTTACACCTTTCAGCTGGTTTACCTCCTAT
GATTCGAGTGGATGGCGACATTCGACGAATCAATATGCCTGAGCTTGAGCATAAAGAAGTTCATGCAATGATTTATGACA
TCATGAACGATAAACAGCGCCGTGACTTTGAAGAGTTTCTTGAAGTCGATTTTTCCTTCGAATTGCCCGGCACTGCTCGC
TTCCGTGTTAATGCCTTTAATCAAGACCGTGGAGCTGCTGCTGTATTTAGAACTATTCCCGTTGATATTCTTACCCTAGA
AGACTTAAATTGCCCCCAATCTTTTAAAGAAATCATTAATGTTCCTCGTGGCATTGTGCTGGTAACAGGGCCAACAGGGT
CAGGAAAATCGACCACACTAGCGGCTTTGATGGATTATAAAAACGATAATGTATACGAACACATTCTCACCATTGAAGAC
CCTATCGAATTCGTACATAAAAGTAAAAAATGCCTTATTAATCAACGTGAGGTACACAAGCATACACATGGGTTTAGTGA
AGCCTTACGTGTTGCGCTTCGTGAAGACCCCGACATTATTCTAGTCGGCGAAATGCGAGATCTTGAAACCATTCGCCTTG
CGTTAACCGCGGCAGAAACAGGCCACTTGGTCTTCGGTACTTTACACACCAGCTCTGCTGCCAAAACCATTGACCTTATT
ATTGATGTATTCCCCGAGGGTGAAAAAGGCATGGTTCGCTCCATGTTATCCGAGTCACTTCGTGCTGTTATTGCACAAAC
CTTAATGAAAAAAATTGGTGGTGGACGAATTGCCGCATGGGAAATCATGACCGGCACTGCGGCTATCCGAAACCTCATTC
GAGAAGATAAAATTGCACAAATGTATTCCACCATACAAACCAGTCGTAAAGATGGTATGCAAACCCTCGATCAACACTTA
ACAGAGCTTGTTCAAGCCGGTGTCATTACAAGACAAGATGCCCGAATGAAAGCCGCCGACAAAAAAGCATTTAGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.453

99.71

0.762
  pilT Acinetobacter nosocomialis M2

75.581

99.71

0.754
  pilT Acinetobacter baumannii D1279779

75.581

99.71

0.754
  pilT Acinetobacter baumannii strain A118

75.581

99.71

0.754
  pilT Pseudomonas stutzeri DSM 10701

75.291

99.71

0.751
  pilT Legionella pneumophila strain Lp02

72.384

99.71

0.722
  pilT Legionella pneumophila strain ERS1305867

72.384

99.71

0.722
  pilT Acinetobacter baylyi ADP1

72.384

99.71

0.722
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.246

100

0.672
  pilT Vibrio cholerae strain A1552

67.246

100

0.672
  pilT Neisseria meningitidis 8013

64.74

100

0.649
  pilT Neisseria gonorrhoeae MS11

64.451

100

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.133

100

0.493
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.474

99.13

0.391
  pilU Vibrio cholerae strain A1552

39.589

98.841

0.391

Multiple sequence alignment