Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CPC19_RS01155 Genome accession   NZ_CP023664
Coordinates   218699..219826 (+) Length   375 a.a.
NCBI ID   WP_015004886.1    Uniprot ID   A0AB33Z1A0
Organism   Cycloclasticus sp. PY97N     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 213699..224826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPC19_RS01135 (CPC19_01135) - 215409..215963 (-) 555 WP_016390294.1 YggT family protein -
  CPC19_RS01140 (CPC19_01140) proC 215963..216790 (-) 828 WP_016390293.1 pyrroline-5-carboxylate reductase -
  CPC19_RS01145 (CPC19_01145) - 216824..217513 (-) 690 WP_016390292.1 YggS family pyridoxal phosphate-dependent enzyme -
  CPC19_RS01150 (CPC19_01150) pilT 217637..218674 (+) 1038 WP_096910734.1 type IV pilus twitching motility protein PilT Machinery gene
  CPC19_RS01155 (CPC19_01155) pilU 218699..219826 (+) 1128 WP_015004886.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CPC19_RS01160 (CPC19_01160) - 219994..220422 (-) 429 WP_016390291.1 type IV pilin protein -
  CPC19_RS01165 (CPC19_01165) - 220598..221122 (+) 525 WP_016390290.1 GspH/FimT family pseudopilin -
  CPC19_RS01170 (CPC19_01170) - 221116..221772 (+) 657 WP_016390289.1 PilW family protein -
  CPC19_RS01175 (CPC19_01175) - 221778..223091 (+) 1314 WP_016390288.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41741.52 Da        Isoelectric Point: 5.0659

>NTDB_id=248981 CPC19_RS01155 WP_015004886.1 218699..219826(+) (pilU) [Cycloclasticus sp. PY97N]
MDFDALLQLVVHKKASDLFITSGWAPTIKVNGALQQVSKTRLSASQSLDIVKSIMTDDQKKEFEETKECQFAIQRPDIGR
FRVSAFIQKDDAGMVLRRIETTIPTSEELNLPPILDELAMTKRGLIIFVGATGTGKSTSLAAMVGYRNRNSSGHIITIED
PVEYVHEHAGCVITQREVGIDTESYEVALKNTLRQAPDVILIGEIRSRETMEHAMTFAETGHLCLSTLHANNANQALDRI
IHFFPEEAHKQLFMDLSLNLKAMIAQQLVPTIDGKGRKAVIEIMLNTPLASDFIRKGDIHKLKELMKNSTEQGMQTFDQA
LYGFYASGDISYENAINYADSSNEVRLMIKLGDAKAKNPDDDNDSGMELIDNYDG

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=248981 CPC19_RS01155 WP_015004886.1 218699..219826(+) (pilU) [Cycloclasticus sp. PY97N]
ATGGATTTTGATGCACTTTTACAACTCGTTGTTCATAAAAAAGCATCTGACTTATTTATTACATCTGGTTGGGCTCCCAC
CATTAAGGTCAATGGAGCGCTACAGCAAGTATCAAAAACACGCTTAAGCGCTAGCCAGTCTTTAGACATCGTAAAAAGCA
TCATGACCGATGATCAGAAAAAAGAGTTTGAAGAGACCAAAGAGTGCCAATTTGCCATTCAACGCCCAGATATTGGCCGT
TTTCGTGTCAGCGCATTTATACAAAAAGACGATGCTGGCATGGTCTTACGTCGAATCGAAACCACCATCCCAACCTCTGA
AGAACTCAACCTTCCACCAATTTTAGACGAATTGGCAATGACAAAACGCGGGCTCATTATCTTCGTGGGCGCAACCGGCA
CCGGTAAATCAACGTCCTTAGCTGCGATGGTTGGCTACCGTAATAGAAACAGTTCCGGCCATATCATCACCATTGAAGAC
CCTGTTGAGTATGTGCATGAACACGCGGGGTGTGTCATTACGCAACGCGAAGTAGGCATAGATACCGAATCGTATGAAGT
TGCGCTTAAAAACACGTTGCGTCAAGCACCCGATGTCATCCTAATTGGTGAGATTCGTAGTCGCGAAACCATGGAGCACG
CTATGACCTTCGCTGAAACGGGGCATTTATGCCTGTCCACTTTGCACGCTAATAACGCCAACCAAGCACTTGATCGTATT
ATTCATTTCTTCCCTGAAGAAGCGCACAAGCAATTATTTATGGATTTATCGCTCAACCTAAAGGCCATGATCGCGCAGCA
ACTTGTACCCACCATCGATGGCAAAGGCAGAAAAGCAGTGATTGAAATTATGCTTAACACACCGTTAGCCTCTGACTTCA
TCAGAAAAGGTGATATTCATAAACTAAAAGAGCTGATGAAAAACTCTACCGAACAAGGCATGCAAACGTTTGACCAGGCA
CTGTACGGCTTCTATGCCAGCGGCGACATCAGCTACGAAAATGCCATTAATTACGCCGACTCCAGCAACGAGGTGCGCTT
AATGATCAAGCTGGGTGATGCAAAAGCTAAAAACCCCGATGATGATAACGACAGTGGTATGGAGTTGATTGATAATTATG
ATGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.898

93.867

0.637
  pilU Acinetobacter baylyi ADP1

62.169

100

0.627
  pilU Vibrio cholerae strain A1552

57.429

93.333

0.536
  pilT Legionella pneumophila strain ERS1305867

41.935

90.933

0.381
  pilT Legionella pneumophila strain Lp02

41.935

90.933

0.381
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.298

90.4

0.373
  pilT Pseudomonas aeruginosa PAK

41.194

89.333

0.368
  pilT Acinetobacter baylyi ADP1

40.176

90.933

0.365
  pilT Vibrio cholerae strain A1552

41.768

87.467

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.768

87.467

0.365
  pilT Acinetobacter baumannii strain A118

40.299

89.333

0.36
  pilT Pseudomonas stutzeri DSM 10701

40.299

89.333

0.36
  pilT Acinetobacter baumannii D1279779

40.299

89.333

0.36
  pilT Neisseria meningitidis 8013

39.474

91.2

0.36
  pilT Neisseria gonorrhoeae MS11

39.474

91.2

0.36

Multiple sequence alignment