Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CHY_RS02830 Genome accession   NC_007503
Coordinates   546099..547187 (+) Length   362 a.a.
NCBI ID   WP_011343563.1    Uniprot ID   Q3AEE7
Organism   Carboxydothermus hydrogenoformans Z-2901     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541099..552187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHY_RS02815 (CHY_0628) - 541344..542249 (-) 906 WP_011343560.1 selenium metabolism-associated LysR family transcriptional regulator -
  CHY_RS02820 (CHY_0629) aroB 542719..544308 (+) 1590 WP_011343561.1 3-dehydroquinate synthase -
  CHY_RS02825 (CHY_0630) - 544371..546077 (+) 1707 WP_011343562.1 GspE/PulE family protein -
  CHY_RS02830 (CHY_0631) pilT 546099..547187 (+) 1089 WP_011343563.1 type IV pilus twitching motility protein PilT Machinery gene
  CHY_RS02835 (CHY_0632) - 547285..548493 (+) 1209 WP_011343564.1 type II secretion system F family protein -
  CHY_RS12610 (CHY_0633) - 548579..549001 (+) 423 WP_011343565.1 type II secretion system protein GspG -
  CHY_RS02845 (CHY_0634) - 549064..549813 (+) 750 WP_011343566.1 prepilin peptidase -
  CHY_RS02850 (CHY_0635) - 549786..550238 (+) 453 WP_011343567.1 pilus assembly FimT family protein -
  CHY_RS02855 (CHY_0636) - 550247..550621 (+) 375 WP_083757246.1 type IV pilus modification PilV family protein -
  CHY_RS02860 (CHY_0637) - 550626..551138 (+) 513 WP_011343569.1 PilW family protein -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 40390.73 Da        Isoelectric Point: 7.6191

>NTDB_id=24873 CHY_RS02830 WP_011343563.1 546099..547187(+) (pilT) [Carboxydothermus hydrogenoformans Z-2901]
MINELLRLMVEFKASDLHLTVNFPPAYRIHGNIRPLTELARENPGIPQSLTRLLTMSDTETMARTIMSTEQWEKFAKMGE
LDFAYSLPDVGRFRVNVFRQRGAVALVLRHINSKILSFKELGLPEVIADLSRKNRGIVLVTGPTGSGKSTTLASMIDLIN
SERACHIITLEDPIEYLHTHKKSVVNQREIGSDTESFAKALRAAMREDPDVILVGEMRDLETISIAITAAETGHLVFATL
HTSSAAETIDRIIDVFPPSQQQQIRVQLSTTIQGIIAQQLIPRMDGKGRVVAVEVMVATPAIRNLIREGKTHQIPAQIQT
GAKYGMQTLDMALAKLYQNRLISYEEAISRAHDPESLARMLY

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=24873 CHY_RS02830 WP_011343563.1 546099..547187(+) (pilT) [Carboxydothermus hydrogenoformans Z-2901]
ATGATTAATGAGCTTTTACGGTTAATGGTAGAGTTTAAAGCGTCGGACTTGCATTTAACGGTGAACTTTCCCCCGGCTTA
CCGGATTCACGGCAATATCCGGCCGCTAACGGAGCTTGCCAGGGAAAATCCGGGGATACCGCAAAGCTTGACAAGGCTTT
TAACCATGAGCGATACCGAAACCATGGCCCGGACGATTATGTCGACCGAGCAGTGGGAAAAATTTGCGAAAATGGGTGAA
CTGGACTTTGCCTACTCTCTACCGGATGTGGGCCGTTTTCGGGTAAACGTCTTTCGCCAGCGAGGAGCGGTAGCTTTAGT
TCTCCGGCATATTAATTCGAAAATATTGTCCTTTAAAGAGTTGGGCCTTCCGGAAGTTATTGCCGATCTGTCCCGGAAAA
ATCGGGGGATTGTCCTGGTAACCGGTCCCACCGGTAGCGGTAAATCAACCACCCTGGCTTCAATGATTGATTTAATCAAT
AGCGAGCGAGCCTGTCACATCATAACCCTGGAAGATCCGATTGAATATCTGCATACCCATAAAAAAAGTGTGGTAAACCA
GCGGGAAATAGGCAGTGATACCGAAAGCTTTGCCAAGGCTTTGCGGGCTGCGATGCGGGAAGATCCGGACGTCATCCTTG
TGGGTGAGATGCGGGACTTAGAAACTATTTCTATCGCGATTACGGCGGCAGAAACTGGACACCTGGTTTTTGCGACCCTG
CACACTTCCAGTGCTGCGGAAACCATTGACCGGATCATAGACGTTTTCCCCCCTTCCCAGCAGCAGCAAATTCGCGTACA
GCTTTCCACCACCATTCAGGGGATAATTGCCCAGCAGTTAATTCCCCGGATGGATGGTAAAGGCCGGGTAGTGGCGGTGG
AGGTCATGGTGGCAACTCCAGCAATAAGAAACCTTATCCGGGAGGGTAAAACCCACCAAATCCCTGCCCAGATTCAAACC
GGGGCTAAATACGGAATGCAGACGTTAGATATGGCATTAGCGAAACTTTATCAAAATCGGCTTATTTCTTATGAAGAAGC
TATTTCCCGGGCCCATGATCCCGAAAGTTTAGCCCGAATGTTATATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3AEE7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.811

99.171

0.514
  pilT Pseudomonas aeruginosa PAK

50.562

98.343

0.497
  pilT Acinetobacter baumannii strain A118

50

98.343

0.492
  pilT Acinetobacter nosocomialis M2

50

98.343

0.492
  pilT Acinetobacter baumannii D1279779

50

98.343

0.492
  pilT Acinetobacter baylyi ADP1

49.719

98.343

0.489
  pilT Legionella pneumophila strain ERS1305867

49.153

97.79

0.481
  pilT Legionella pneumophila strain Lp02

49.153

97.79

0.481
  pilT Vibrio cholerae strain A1552

49.857

96.409

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.857

96.409

0.481
  pilT Neisseria meningitidis 8013

48.468

99.171

0.481
  pilT Pseudomonas stutzeri DSM 10701

48.876

98.343

0.481
  pilT Neisseria gonorrhoeae MS11

48.189

99.171

0.478
  pilU Pseudomonas stutzeri DSM 10701

42.737

98.895

0.423
  pilU Vibrio cholerae strain A1552

41.389

99.448

0.412
  pilU Acinetobacter baylyi ADP1

41.176

98.619

0.406

Multiple sequence alignment