Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CCZ27_RS22250 Genome accession   NZ_CP023439
Coordinates   5101916..5102959 (-) Length   347 a.a.
NCBI ID   WP_096451922.1    Uniprot ID   -
Organism   Thauera sp. K11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5096916..5107959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCZ27_RS22230 (CCZ27_22230) - 5096917..5098506 (-) 1590 WP_232516500.1 FecR domain-containing protein -
  CCZ27_RS22235 (CCZ27_22235) mobA 5098747..5099304 (-) 558 WP_232516692.1 molybdenum cofactor guanylyltransferase MobA -
  CCZ27_RS22240 (CCZ27_22240) - 5099531..5100703 (+) 1173 WP_096451918.1 THUMP domain-containing protein -
  CCZ27_RS22245 (CCZ27_22245) pilU 5100711..5101844 (-) 1134 WP_096451920.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CCZ27_RS22250 (CCZ27_22250) pilT 5101916..5102959 (-) 1044 WP_096451922.1 type IV pilus twitching motility protein PilT Machinery gene
  CCZ27_RS22255 (CCZ27_22255) - 5103051..5103758 (+) 708 WP_096451924.1 YggS family pyridoxal phosphate-dependent enzyme -
  CCZ27_RS22260 (CCZ27_22260) proC 5103841..5104665 (+) 825 WP_096451926.1 pyrroline-5-carboxylate reductase -
  CCZ27_RS22265 (CCZ27_22265) - 5104673..5105254 (+) 582 WP_096451928.1 YggT family protein -
  CCZ27_RS22270 (CCZ27_22270) - 5105259..5105570 (+) 312 WP_096451930.1 DUF167 family protein -
  CCZ27_RS22275 (CCZ27_22275) - 5105572..5106852 (-) 1281 WP_096451932.1 dihydroorotase -
  CCZ27_RS22280 (CCZ27_22280) - 5106863..5107822 (-) 960 WP_096451934.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38576.17 Da        Isoelectric Point: 6.8320

>NTDB_id=247174 CCZ27_RS22250 WP_096451922.1 5101916..5102959(-) (pilT) [Thauera sp. K11]
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKDVHAMVYDIMNDAQRKQYEESWECDFSFAVPNLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEELNCPKIFKEIADQPRGIVLVTGPTGSGKSTTLAAMVDYINENEYGHILTVED
PIEFVHESKRSLINQREVHRDTMSFNNALRAALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGAGRVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQNVGMQTLDQC
LADLVRRNVVSAAEARVRAQNKDNFAS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=247174 CCZ27_RS22250 WP_096451922.1 5101916..5102959(-) (pilT) [Thauera sp. K11]
ATGGACATCACCGAACTGCTCGCCTTCGCGGTCAAGAACAAGGCTTCCGACCTCCACCTCTCGGCCGGCCTTCCGCCGAT
GATCCGCGTGCACGGCGACGTGCGCCGCATCAACCTGCCGCCCATGGAACACAAGGACGTGCACGCCATGGTGTACGACA
TCATGAACGATGCGCAGCGCAAGCAGTACGAGGAGAGCTGGGAGTGCGACTTCTCGTTCGCGGTGCCCAACCTCGCGCGC
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCGGGCGCGGTGTTCCGGACCATTCCGTCCAAGGTGCTGACGCTCGA
GGAACTCAACTGCCCGAAGATCTTCAAGGAGATCGCCGACCAGCCGCGCGGCATCGTGCTCGTCACCGGGCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGACTACATCAACGAGAACGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCATGAATCCAAGCGCAGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGATGTCCTTCAACAA
CGCGCTGCGCGCCGCGCTGCGCGAGGACCCGGACGTGATCCTGGTGGGCGAAATGCGCGACCTCGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGCATC
GTCGACGTCTTCCCGGCGGCGGAAAAGGACATGGTGCGCTCCATGCTGTCGGAATCGCTGCGCGCAGTGATCTCGCAGAC
GCTGCTGAAGACCAAGGACGGCGCCGGCCGGGTGGCGGCGCACGAGATCATGATCGGCACCCCCGCCATCCGCAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGTCGATCCAGACCGGCCAGAACGTGGGCATGCAGACGCTGGACCAGTGC
CTGGCCGATCTCGTGCGGCGCAACGTGGTGTCGGCCGCCGAGGCCCGCGTGCGTGCGCAGAACAAGGATAATTTCGCCAG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.29

99.424

0.798
  pilT Acinetobacter baumannii strain A118

80.29

99.424

0.798
  pilT Acinetobacter nosocomialis M2

80.29

99.424

0.798
  pilT Acinetobacter baylyi ADP1

77.391

99.424

0.769
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Legionella pneumophila strain Lp02

73.913

99.424

0.735
  pilT Legionella pneumophila strain ERS1305867

73.913

99.424

0.735
  pilT Neisseria meningitidis 8013

71.676

99.712

0.715
  pilT Neisseria gonorrhoeae MS11

71.387

99.712

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.824

97.983

0.674
  pilT Vibrio cholerae strain A1552

68.824

97.983

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.468

99.712

0.533
  pilU Acinetobacter baylyi ADP1

41.379

100

0.415
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.58

99.424

0.403

Multiple sequence alignment