Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CCZ27_RS22245 Genome accession   NZ_CP023439
Coordinates   5100711..5101844 (-) Length   377 a.a.
NCBI ID   WP_096451920.1    Uniprot ID   -
Organism   Thauera sp. K11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5095711..5106844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCZ27_RS22230 (CCZ27_22230) - 5096917..5098506 (-) 1590 WP_232516500.1 FecR domain-containing protein -
  CCZ27_RS22235 (CCZ27_22235) mobA 5098747..5099304 (-) 558 WP_232516692.1 molybdenum cofactor guanylyltransferase MobA -
  CCZ27_RS22240 (CCZ27_22240) - 5099531..5100703 (+) 1173 WP_096451918.1 THUMP domain-containing protein -
  CCZ27_RS22245 (CCZ27_22245) pilU 5100711..5101844 (-) 1134 WP_096451920.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CCZ27_RS22250 (CCZ27_22250) pilT 5101916..5102959 (-) 1044 WP_096451922.1 type IV pilus twitching motility protein PilT Machinery gene
  CCZ27_RS22255 (CCZ27_22255) - 5103051..5103758 (+) 708 WP_096451924.1 YggS family pyridoxal phosphate-dependent enzyme -
  CCZ27_RS22260 (CCZ27_22260) proC 5103841..5104665 (+) 825 WP_096451926.1 pyrroline-5-carboxylate reductase -
  CCZ27_RS22265 (CCZ27_22265) - 5104673..5105254 (+) 582 WP_096451928.1 YggT family protein -
  CCZ27_RS22270 (CCZ27_22270) - 5105259..5105570 (+) 312 WP_096451930.1 DUF167 family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42401.81 Da        Isoelectric Point: 6.5064

>NTDB_id=247173 CCZ27_RS22245 WP_096451920.1 5100711..5101844(-) (pilU) [Thauera sp. K11]
MERDQALKFMHDLLRLMLQKNGSDLFINAGFPPAIKVDGRIVPQSNQPLLPQHTAELARAIMNDRQAAEFEATKECNFAI
SPAGIGRFRANAYVQQGRIALVLRTIPQRIPTFEELGLPRVLSEVAMTKRGLVIFVGGTGTGKTTSLAAMVDYRNQNSYG
HIITVEDPIEFVHAHKNCIVSQRELGIDTDSWESALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRQQLLMDLSLNLRALVSQRLLPMKERKGRVPAVEILLNSPLIADLIFKGDIPSIKDIMKRSRELG
MQTFDQALFELYETELITYEDALRNADSVNDLRLQIKLNSRHGERDMSAGIQNLDIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=247173 CCZ27_RS22245 WP_096451920.1 5100711..5101844(-) (pilU) [Thauera sp. K11]
ATGGAACGCGACCAGGCCCTCAAGTTCATGCACGACCTGCTGCGGCTGATGCTGCAGAAGAACGGCTCGGACCTCTTCAT
CAACGCCGGCTTTCCACCCGCGATCAAGGTCGACGGCCGCATCGTCCCGCAGTCCAACCAGCCGCTGCTGCCCCAGCACA
CGGCGGAACTGGCGCGCGCGATCATGAACGATCGCCAGGCGGCCGAGTTCGAGGCCACCAAGGAATGCAACTTCGCCATC
TCGCCGGCGGGCATCGGCCGCTTCCGCGCCAACGCCTACGTGCAGCAGGGCCGCATCGCACTGGTGCTGCGCACGATTCC
GCAGCGCATCCCGACCTTCGAGGAACTGGGCCTGCCGCGGGTGCTGAGCGAGGTGGCGATGACCAAGCGCGGGCTGGTGA
TCTTCGTCGGCGGCACCGGCACGGGCAAGACGACCTCGCTGGCGGCGATGGTCGATTACCGCAACCAGAACTCCTATGGC
CACATCATCACCGTCGAGGATCCGATCGAGTTCGTGCACGCGCACAAGAACTGCATCGTGTCGCAGCGCGAGCTGGGTAT
CGACACCGACTCCTGGGAAAGCGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACGATGGACTACGCGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGATCGACCGCATCATCAACTTCTTCCCCGAGGACCGGCGCCAGCAACTGCTGATGGACCTGTCGCTGAACCT
GCGCGCGCTGGTGTCGCAGCGCCTGCTGCCGATGAAGGAGCGCAAGGGCCGCGTGCCGGCGGTGGAGATCCTGCTCAACT
CGCCGCTGATCGCCGACCTGATCTTCAAGGGCGACATCCCGAGCATCAAGGACATCATGAAACGCTCGCGCGAGCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGAACTCTACGAGACCGAACTCATCACCTACGAGGATGCGCTGCGCAATGCCGA
CTCGGTGAACGACCTGCGCTTGCAGATCAAGCTCAACAGCCGGCACGGCGAGCGCGACATGAGCGCCGGCATCCAGAACC
TGGACATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.637

96.552

0.605
  pilU Acinetobacter baylyi ADP1

58.166

92.573

0.538
  pilU Vibrio cholerae strain A1552

54.722

95.491

0.523
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.478

88.859

0.395
  pilT Legionella pneumophila strain Lp02

44.713

87.798

0.393
  pilT Legionella pneumophila strain ERS1305867

44.713

87.798

0.393
  pilT Acinetobacter baylyi ADP1

42.604

89.655

0.382
  pilT Pseudomonas aeruginosa PAK

42.388

88.859

0.377
  pilT Pseudomonas stutzeri DSM 10701

41.964

89.125

0.374
  pilT Acinetobacter nosocomialis M2

41.867

88.064

0.369
  pilT Acinetobacter baumannii D1279779

41.867

88.064

0.369
  pilT Acinetobacter baumannii strain A118

41.867

88.064

0.369

Multiple sequence alignment