Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CNX70_RS05315 Genome accession   NZ_CP023422
Coordinates   1208486..1209529 (-) Length   347 a.a.
NCBI ID   WP_071079443.1    Uniprot ID   A0A1S1U2E3
Organism   Janthinobacterium svalbardensis strain PAMC 27463     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1203486..1214529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNX70_RS05300 (CNX70_05300) - 1204561..1206672 (-) 2112 WP_096233844.1 EAL domain-containing protein -
  CNX70_RS05305 (CNX70_05305) - 1206856..1207341 (-) 486 WP_096233845.1 glutathione peroxidase -
  CNX70_RS05310 (CNX70_05310) pilU 1207343..1208482 (-) 1140 WP_208624493.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CNX70_RS05315 (CNX70_05315) pilT 1208486..1209529 (-) 1044 WP_071079443.1 type IV pilus twitching motility protein PilT Machinery gene
  CNX70_RS05320 (CNX70_05320) - 1209600..1210304 (+) 705 WP_096233846.1 YggS family pyridoxal phosphate-dependent enzyme -
  CNX70_RS05325 (CNX70_05325) proC 1210323..1211153 (+) 831 WP_096233847.1 pyrroline-5-carboxylate reductase -
  CNX70_RS05330 (CNX70_05330) - 1211161..1211877 (-) 717 WP_096233848.1 anti-sigma factor -
  CNX70_RS05335 (CNX70_05335) - 1211874..1212440 (-) 567 WP_096233849.1 sigma-70 family RNA polymerase sigma factor -
  CNX70_RS05340 (CNX70_05340) - 1212531..1213088 (-) 558 WP_096233850.1 DUF3455 domain-containing protein -
  CNX70_RS05345 (CNX70_05345) - 1213178..1213525 (-) 348 WP_096233851.1 hypothetical protein -
  CNX70_RS05350 (CNX70_05350) - 1213522..1213908 (-) 387 WP_096233852.1 phage holin family protein -
  CNX70_RS05355 (CNX70_05355) - 1213972..1214328 (-) 357 WP_046683402.1 DUF883 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38137.69 Da        Isoelectric Point: 6.6919

>NTDB_id=246964 CNX70_RS05315 WP_071079443.1 1208486..1209529(-) (pilT) [Janthinobacterium svalbardensis strain PAMC 27463]
MDISELLAFSVNNKASDLHLSSGLPPMIRVHGDVRRLNVAPLEHKEVHSMIYDIMNDSQRKAYEEALECDFSFEIPGLAR
FRVNAYNQERGASAVLRTIPSKVLTLEELNAPRIFGELAMRPRGLVLVTGPTGSGKSTTLAAMVNHVNERLNHHILTIED
PIEFVHEPKKCLINQREVGSHTHSFSNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKSIDRI
IDVFPAEEKEMVRAMLSESLQAVISQNLLKTKDGSGRVAAHEIMLATPAVRNLIREAKVAQMYSAIQTGSNVGMQTLDQC
LSDLVRRGTITAETARAAAKVPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=246964 CNX70_RS05315 WP_071079443.1 1208486..1209529(-) (pilT) [Janthinobacterium svalbardensis strain PAMC 27463]
ATGGACATCTCCGAACTACTCGCTTTCTCCGTCAACAACAAGGCTTCCGACCTGCACCTGTCTTCCGGCCTGCCGCCGAT
GATACGCGTCCATGGCGACGTGCGCCGCCTGAACGTGGCGCCGCTCGAGCACAAGGAAGTGCACAGCATGATCTACGACA
TCATGAACGACAGCCAGCGCAAAGCCTATGAAGAAGCGCTGGAATGCGATTTCTCGTTCGAGATTCCCGGCCTGGCGCGC
TTCCGCGTCAACGCCTACAACCAGGAACGGGGCGCTTCGGCCGTGCTGCGCACGATTCCCTCAAAAGTGCTGACCCTGGA
AGAACTCAACGCCCCGCGCATCTTCGGCGAGCTGGCCATGCGTCCGCGGGGACTCGTGCTGGTCACGGGCCCGACGGGTT
CGGGCAAGTCGACCACCCTGGCGGCCATGGTGAACCACGTCAACGAACGCCTGAACCACCATATTCTGACCATCGAAGAC
CCGATCGAATTCGTGCATGAGCCGAAAAAATGCCTGATCAACCAGCGCGAGGTGGGGTCGCACACGCATTCGTTCAGCAA
CGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACGTGATTTTAGTGGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCCGAGACGGGCCACCTCGTGTTCGGCACCCTGCACACGTCGTCGGCGGCGAAATCGATCGACCGCATC
ATCGACGTCTTCCCCGCCGAAGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAATCCTTGCAAGCCGTCATTTCGCAGAA
CTTGCTCAAAACCAAGGACGGCAGCGGCCGCGTGGCCGCGCATGAAATCATGCTGGCCACGCCGGCCGTGCGCAACCTGA
TCCGCGAAGCGAAAGTGGCGCAGATGTATTCGGCCATCCAGACGGGCAGCAACGTGGGCATGCAGACCCTGGACCAGTGC
CTGTCCGACCTGGTGCGGCGCGGCACGATTACGGCGGAGACAGCCCGCGCGGCCGCCAAGGTTCCCGAAAACTTCCCCGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S1U2E3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.362

99.424

0.749
  pilT Acinetobacter nosocomialis M2

75.072

99.424

0.746
  pilT Acinetobacter baumannii D1279779

75.072

99.424

0.746
  pilT Acinetobacter baumannii strain A118

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Pseudomonas stutzeri DSM 10701

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

71.091

97.695

0.695
  pilT Legionella pneumophila strain ERS1305867

71.091

97.695

0.695
  pilT Neisseria meningitidis 8013

69.275

99.424

0.689
  pilT Neisseria gonorrhoeae MS11

68.986

99.424

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.118

0.68
  pilT Vibrio cholerae strain A1552

70.03

97.118

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

99.424

0.524
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Vibrio cholerae strain A1552

41.194

96.542

0.398

Multiple sequence alignment