Detailed information NTG0246963

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CNX70_RS05310
• Genome accession: NZ_CP023422
• Coordinates: 1207343..1208482 (-)
• Length: 379 a.a.
• NCBI ID: WP_208624493.1
• Uniprot ID: -
• Organism: Janthinobacterium svalbardensis strain PAMC 27463
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1202343..1213482

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CNX70_RS05295 (CNX70_05295)	glcF	1203339..1204568 (+)	1230	WP_096233843.1	glycolate oxidase subunit GlcF	-
CNX70_RS05300 (CNX70_05300)	-	1204561..1206672 (-)	2112	WP_096233844.1	EAL domain-containing protein	-
CNX70_RS05305 (CNX70_05305)	-	1206856..1207341 (-)	486	WP_096233845.1	glutathione peroxidase	-
CNX70_RS05310 (CNX70_05310)	pilU	1207343..1208482 (-)	1140	WP_208624493.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CNX70_RS05315 (CNX70_05315)	pilT	1208486..1209529 (-)	1044	WP_071079443.1	type IV pilus twitching motility protein PilT	Machinery gene
CNX70_RS05320 (CNX70_05320)	-	1209600..1210304 (+)	705	WP_096233846.1	YggS family pyridoxal phosphate-dependent enzyme	-
CNX70_RS05325 (CNX70_05325)	proC	1210323..1211153 (+)	831	WP_096233847.1	pyrroline-5-carboxylate reductase	-
CNX70_RS05330 (CNX70_05330)	-	1211161..1211877 (-)	717	WP_096233848.1	anti-sigma factor	-
CNX70_RS05335 (CNX70_05335)	-	1211874..1212440 (-)	567	WP_096233849.1	sigma-70 family RNA polymerase sigma factor	-
CNX70_RS05340 (CNX70_05340)	-	1212531..1213088 (-)	558	WP_096233850.1	DUF3455 domain-containing protein	-

Sequence

Protein

• Length: 379 a.a.
• Molecular weight: 40980.14 Da
• Isoelectric Point: 6.8672

>NTDB_id=246963 CNX70_RS05310 WP_208624493.1 1207343..1208482(-) (pilU) [Janthinobacterium svalbardensis strain PAMC 27463]
MQTTQEHYGAMHALLAQMRARGGSDLFITAGFPPAIKLDGKLTPLVGTALDTQQAAGYVRAVMNERQAAEFASSREANFA
ISPDGLGRFRVSAFVQMGQAGMVLRLINTAIPTLDGLGLPAILQDIVMSKRGLVIMVGATGCGKSTTLAAMVGHRNAHSH
GHIITIEDPVEFIHPHGNCIVTQREVGVDTDDWATALKNTLRQAPDVIQIGEIRDRETMDHAIAFAETGHLCLATLHANN
ANQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLIPNKEGGGRKAALEILLNSPLMSDLIFKGQVHEIKELMKKSREL
GMQTFDQALFDLHEAGAVSHEDALRNADSVNDLRLAIKLKGATAPAPAPQTGTTTLGLL

Nucleotide

• Length: 1140 bp

>NTDB_id=246963 CNX70_RS05310 WP_208624493.1 1207343..1208482(-) (pilU) [Janthinobacterium svalbardensis strain PAMC 27463]
ATGCAAACGACGCAAGAACACTATGGCGCCATGCACGCGCTGCTGGCGCAAATGCGCGCACGGGGCGGCTCGGACCTGTT
CATCACGGCCGGCTTTCCGCCCGCCATCAAGCTCGACGGCAAGCTGACGCCGCTGGTAGGCACCGCGCTCGATACGCAGC
AGGCGGCCGGCTACGTGCGCGCCGTCATGAATGAACGCCAGGCGGCCGAATTCGCGTCCAGCCGCGAAGCCAATTTCGCC
ATCAGCCCGGACGGCCTGGGGCGTTTCCGCGTGTCCGCCTTCGTGCAGATGGGCCAGGCAGGCATGGTCTTGCGCTTGAT
CAATACCGCCATCCCCACGCTGGATGGATTGGGCCTGCCCGCCATCCTGCAAGACATCGTGATGAGCAAGCGGGGCCTCG
TCATCATGGTGGGCGCCACGGGCTGCGGCAAGTCGACCACGCTGGCAGCCATGGTGGGCCACCGCAACGCCCACAGCCAC
GGCCACATCATCACCATCGAAGATCCTGTGGAATTCATCCATCCGCATGGTAACTGCATCGTCACCCAGCGCGAGGTGGG
CGTCGATACGGACGACTGGGCCACGGCCCTGAAAAACACCCTGCGCCAGGCGCCCGACGTGATCCAGATCGGCGAAATCC
GCGACCGCGAGACCATGGACCACGCCATCGCATTCGCCGAGACGGGCCATCTGTGCCTGGCGACCCTGCACGCGAACAAC
GCCAACCAGGCGCTGGACCGCATCATCAATTTCTTCCCCGAAGAGCGGCGCCAGCAGCTGCTGATGGATCTGTCGCTCAA
TTTGAAGGGCATGATTTCGCAGCGGCTGATCCCCAACAAGGAAGGCGGCGGGCGCAAGGCGGCGCTGGAAATCCTGCTCA
ATTCGCCGCTGATGAGCGACCTGATCTTCAAGGGCCAGGTGCACGAGATCAAGGAGCTGATGAAAAAATCGCGCGAGCTT
GGCATGCAGACGTTCGACCAGGCCCTGTTCGACCTGCACGAAGCGGGCGCCGTCAGCCATGAAGATGCGCTGCGCAATGC
GGACTCCGTCAACGACCTGCGCCTGGCGATCAAGCTCAAGGGCGCAACGGCGCCTGCACCGGCCCCGCAAACGGGCACCA
CCACGCTGGGACTGCTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (88-278)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	64	92.348	0.591
pilU	Acinetobacter baylyi ADP1	59.942	91.557	0.549
pilU	Vibrio cholerae strain A1552	56	92.348	0.517
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.373	87.599	0.38