Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TBD_RS12140 Genome accession   NC_007404
Coordinates   2490089..2491132 (+) Length   347 a.a.
NCBI ID   WP_011312931.1    Uniprot ID   Q3SG78
Organism   Thiobacillus denitrificans ATCC 25259     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2485089..2496132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TBD_RS12120 (Tbd_2415) - 2485796..2487700 (-) 1905 WP_041432751.1 dynamin family protein -
  TBD_RS12125 (Tbd_2416) - 2487908..2488483 (-) 576 WP_011312928.1 YggT family protein -
  TBD_RS12130 (Tbd_2417) proC 2488480..2489295 (-) 816 WP_011312929.1 pyrroline-5-carboxylate reductase -
  TBD_RS12135 (Tbd_2418) - 2489299..2490039 (-) 741 WP_011312930.1 YggS family pyridoxal phosphate-dependent enzyme -
  TBD_RS12140 (Tbd_2419) pilT 2490089..2491132 (+) 1044 WP_011312931.1 type IV pilus twitching motility protein PilT Machinery gene
  TBD_RS12145 (Tbd_2420) pilU 2491156..2492283 (+) 1128 WP_041433238.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TBD_RS12150 (Tbd_2421) - 2492381..2493094 (-) 714 WP_011312933.1 AbiV family abortive infection protein -
  TBD_RS12155 (Tbd_2422) - 2493248..2493634 (-) 387 WP_011312934.1 type II toxin-antitoxin system VapC family toxin -
  TBD_RS12160 (Tbd_2423) - 2493631..2493879 (-) 249 WP_011312935.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  TBD_RS12165 (Tbd_2424) - 2493960..2495156 (-) 1197 WP_011312936.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  TBD_RS12170 (Tbd_2425) - 2495263..2495676 (-) 414 WP_011312937.1 CopD family protein -
  TBD_RS15180 (Tbd_2426) - 2495675..2496049 (+) 375 WP_238376455.1 TlpA disulfide reductase family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38494.20 Da        Isoelectric Point: 6.7738

>NTDB_id=24680 TBD_RS12140 WP_011312931.1 2490089..2491132(+) (pilT) [Thiobacillus denitrificans ATCC 25259]
MDISELLAFAVKNKASDLHLSSGLPPMIRVNGDIRRINLPPMEHKDVHRMVYDIMNDSQRKVYEETLEIDFSFEIPSLAR
FRVNAFNQQYGAGAVFRTIPSKVLTLEELNCPPIFKEIADQPRGIVLVTGPTGSGKSTTLAAMMDYVNENQYSHILTVED
PIEFVHQSKKCLINQREVGPHTLSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGTGRVAAHEIMVGTPAIRNLIRENKVAQMYSAIQTGGNLGMQTLDQN
LQDLVRRNVIASNVARSAAQNKDAFML

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=24680 TBD_RS12140 WP_011312931.1 2490089..2491132(+) (pilT) [Thiobacillus denitrificans ATCC 25259]
GTGGATATTTCTGAATTGCTCGCTTTCGCCGTCAAAAACAAGGCTTCCGACCTGCACCTTTCCTCGGGTCTGCCGCCGAT
GATCCGCGTGAACGGCGACATCCGCCGCATCAACCTGCCGCCGATGGAGCACAAGGACGTGCACCGCATGGTGTACGACA
TCATGAACGACAGCCAGCGCAAGGTGTACGAGGAGACGCTCGAAATCGACTTCTCGTTCGAGATACCCTCGCTCGCGCGC
TTTCGCGTCAACGCGTTCAACCAACAATACGGCGCGGGCGCCGTGTTCCGCACGATTCCGTCGAAGGTGCTGACGCTCGA
AGAACTGAACTGCCCGCCGATCTTCAAGGAAATCGCCGACCAGCCGCGCGGCATCGTGCTCGTCACCGGGCCGACGGGTT
CGGGCAAGTCGACGACGCTCGCGGCGATGATGGACTACGTCAACGAGAACCAGTATTCGCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACCAGAGCAAGAAATGCCTGATCAACCAGCGCGAGGTCGGGCCGCACACGCTCTCGTTCAACAA
CGCCTTGCGTTCCGCGCTGCGTGAAGACCCCGACGTCATCCTGGTCGGCGAATTGCGCGACCTCGAAACGATCCGCCTCG
CGCTGACCGCTGCCGAGACGGGCCACCTCGTCTTCGGCACGCTCCACACCTCGTCGGCGGCCAAGACGATCGACCGCGTC
GTCGACGTCTTCCCCGCTGCGGAAAAGGAAATGGTCCGCTCGATGCTCTCGGAATCGCTGCGCGCGGTGATTTCGCAGAC
GCTGCTGAAGACCAAGGACGGCACCGGCCGCGTCGCCGCGCACGAAATCATGGTCGGCACCCCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGGTCGCACAGATGTATTCGGCGATCCAGACCGGCGGCAACCTCGGCATGCAGACCCTCGACCAGAAC
CTGCAGGATCTCGTGCGCCGCAACGTCATCGCGTCGAACGTGGCCCGCTCCGCGGCGCAGAACAAAGACGCCTTCATGCT
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3SG78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.191

99.712

0.79
  pilT Acinetobacter baumannii D1279779

79.191

99.712

0.79
  pilT Acinetobacter baumannii strain A118

79.191

99.712

0.79
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

73.913

99.424

0.735
  pilT Legionella pneumophila strain ERS1305867

73.913

99.424

0.735
  pilT Neisseria meningitidis 8013

72.174

99.424

0.718
  pilT Neisseria gonorrhoeae MS11

71.594

99.424

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.118

0.666
  pilT Vibrio cholerae strain A1552

68.546

97.118

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.03

95.101

0.504
  pilU Pseudomonas stutzeri DSM 10701

42.901

93.372

0.401
  pilU Vibrio cholerae strain A1552

39.769

100

0.398
  pilU Acinetobacter baylyi ADP1

42.154

93.66

0.395

Multiple sequence alignment