Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   TBD_RS12145 Genome accession   NC_007404
Coordinates   2491156..2492283 (+) Length   375 a.a.
NCBI ID   WP_041433238.1    Uniprot ID   -
Organism   Thiobacillus denitrificans ATCC 25259     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2486156..2497283
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TBD_RS12125 (Tbd_2416) - 2487908..2488483 (-) 576 WP_011312928.1 YggT family protein -
  TBD_RS12130 (Tbd_2417) proC 2488480..2489295 (-) 816 WP_011312929.1 pyrroline-5-carboxylate reductase -
  TBD_RS12135 (Tbd_2418) - 2489299..2490039 (-) 741 WP_011312930.1 YggS family pyridoxal phosphate-dependent enzyme -
  TBD_RS12140 (Tbd_2419) pilT 2490089..2491132 (+) 1044 WP_011312931.1 type IV pilus twitching motility protein PilT Machinery gene
  TBD_RS12145 (Tbd_2420) pilU 2491156..2492283 (+) 1128 WP_041433238.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TBD_RS12150 (Tbd_2421) - 2492381..2493094 (-) 714 WP_011312933.1 AbiV family abortive infection protein -
  TBD_RS12155 (Tbd_2422) - 2493248..2493634 (-) 387 WP_011312934.1 type II toxin-antitoxin system VapC family toxin -
  TBD_RS12160 (Tbd_2423) - 2493631..2493879 (-) 249 WP_011312935.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  TBD_RS12165 (Tbd_2424) - 2493960..2495156 (-) 1197 WP_011312936.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  TBD_RS12170 (Tbd_2425) - 2495263..2495676 (-) 414 WP_011312937.1 CopD family protein -
  TBD_RS15180 (Tbd_2426) - 2495675..2496049 (+) 375 WP_238376455.1 TlpA disulfide reductase family protein -
  TBD_RS15185 - 2496022..2496231 (+) 210 WP_049750256.1 hypothetical protein -
  TBD_RS12180 (Tbd_2427) - 2496228..2496431 (+) 204 WP_011312939.1 SlyX family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41892.12 Da        Isoelectric Point: 6.4358

>NTDB_id=24681 TBD_RS12145 WP_041433238.1 2491156..2492283(+) (pilU) [Thiobacillus denitrificans ATCC 25259]
MNAEKTVHDLLRLMLAKNASDLFITAGFPPAIKVDGKITPVSNQSLTPAHTSELARSIMNDRQWKDFEATNESNFAISPP
EIGRFRVNVFVQQGRVGIVMRTINTKIPKMEDLNLPPILRDVAMIKRGLVVFVGGTGTGKSTSLAAMVGYRNENDAGHII
TVEDPIEYVHEHRKSIVSQREVGIDTDNWFSALKNTLRQAPDVILIGEIRDRETMEYAIAFAETGHLCLSTLHANSANQA
LDRIINFFPEEKRDQLLMDLSLNLKSFISQRLIPRKGSNGRVAAVEILLNSPLIADLIFKGHVHEIKEIMAKSRELGMQT
FDQALFDLYEAGLISYEDALRNADSLNDLRLQIKLHGQEAKGRDMMAGLDHLDIV

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=24681 TBD_RS12145 WP_041433238.1 2491156..2492283(+) (pilU) [Thiobacillus denitrificans ATCC 25259]
ATGAACGCAGAAAAAACCGTCCACGACCTGCTGCGCCTGATGCTGGCGAAGAACGCGTCCGACCTTTTCATCACCGCGGG
ATTCCCGCCCGCGATCAAGGTCGACGGCAAGATCACCCCGGTCTCGAACCAGAGCCTGACGCCCGCGCACACGAGCGAGC
TCGCGCGCTCGATCATGAACGATCGCCAGTGGAAAGATTTCGAGGCGACCAACGAATCGAACTTCGCGATCAGCCCGCCC
GAGATCGGGCGCTTCCGCGTCAACGTCTTCGTGCAACAGGGCCGCGTCGGCATCGTCATGCGGACGATCAATACCAAGAT
CCCGAAGATGGAAGACCTCAACCTGCCGCCGATCCTGCGCGACGTCGCGATGATCAAGCGCGGCCTCGTCGTCTTCGTCG
GCGGCACCGGCACCGGTAAATCGACCTCGCTCGCCGCGATGGTCGGCTACCGCAACGAGAACGACGCCGGCCACATCATC
ACCGTCGAGGACCCGATCGAATACGTGCACGAGCACCGCAAATCGATCGTCAGCCAGCGCGAGGTCGGCATCGACACCGA
CAACTGGTTCTCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTCATCCTGATCGGCGAAATCCGCGACCGGGAGA
CCATGGAATACGCGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGTCTACGCTGCACGCCAACAGCGCCAACCAGGCG
CTCGACCGCATCATCAACTTCTTCCCCGAGGAGAAGCGCGATCAATTGCTGATGGACTTGTCGCTCAACCTCAAGTCCTT
CATCTCGCAGCGCCTGATTCCGCGAAAAGGCAGCAACGGCCGCGTCGCCGCCGTCGAAATCCTGCTCAACTCGCCGCTCA
TCGCCGACCTGATCTTCAAGGGCCACGTCCACGAGATCAAGGAGATCATGGCCAAGTCCCGCGAACTCGGCATGCAGACC
TTCGACCAGGCCCTGTTCGACCTCTACGAAGCCGGACTGATCTCCTACGAGGACGCGCTAAGAAACGCCGACAGCCTCAA
CGACCTACGCCTGCAGATCAAGCTGCACGGCCAGGAGGCCAAGGGGCGGGACATGATGGCGGGGTTGGATCATCTCGATA
TTGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.732

95.467

0.589
  pilU Acinetobacter baylyi ADP1

59.384

95.2

0.565
  pilU Vibrio cholerae strain A1552

56.305

90.933

0.512
  pilT Pseudomonas aeruginosa PAK

44.214

89.867

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.44

91.467

0.397
  pilT Acinetobacter baylyi ADP1

43.363

90.4

0.392
  pilT Acinetobacter baumannii strain A118

43.844

88.8

0.389
  pilT Acinetobacter nosocomialis M2

43.844

88.8

0.389
  pilT Acinetobacter baumannii D1279779

43.844

88.8

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.867

0.387
  pilT Legionella pneumophila strain Lp02

42.342

88.8

0.376
  pilT Legionella pneumophila strain ERS1305867

42.342

88.8

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

88.533

0.373
  pilT Vibrio cholerae strain A1552

42.169

88.533

0.373

Multiple sequence alignment