Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CKY39_RS29810 Genome accession   NZ_CP023284
Coordinates   6468828..6469871 (+) Length   347 a.a.
NCBI ID   WP_062482334.1    Uniprot ID   A0A554RS02
Organism   Variovorax boronicumulans strain J1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6463828..6474871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKY39_RS34610 - 6464128..6464634 (+) 507 WP_240531224.1 ATP-binding cassette domain-containing protein -
  CKY39_RS34615 - 6464598..6464942 (+) 345 WP_240531225.1 hypothetical protein -
  CKY39_RS29785 (CKY39_29785) dapA 6464986..6465879 (+) 894 WP_095746985.1 4-hydroxy-tetrahydrodipicolinate synthase -
  CKY39_RS29790 (CKY39_29790) - 6465886..6466728 (+) 843 WP_095746986.1 alpha/beta hydrolase -
  CKY39_RS29795 (CKY39_29795) - 6466725..6467315 (+) 591 WP_095746987.1 malonic semialdehyde reductase -
  CKY39_RS29800 (CKY39_29800) - 6467326..6468062 (+) 737 Protein_6024 sulfite exporter TauE/SafE family protein -
  CKY39_RS29805 (CKY39_29805) - 6468075..6468776 (-) 702 WP_095746988.1 YggS family pyridoxal phosphate-dependent enzyme -
  CKY39_RS29810 (CKY39_29810) pilT 6468828..6469871 (+) 1044 WP_062482334.1 type IV pilus twitching motility protein PilT Machinery gene
  CKY39_RS29815 (CKY39_29815) pilU 6469901..6471037 (+) 1137 WP_095746989.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CKY39_RS29820 (CKY39_29820) - 6471106..6472014 (+) 909 WP_095746990.1 NAD(P)-dependent oxidoreductase -
  CKY39_RS29825 (CKY39_29825) - 6472045..6472839 (-) 795 WP_062482325.1 BON domain-containing protein -
  CKY39_RS29830 (CKY39_29830) - 6472836..6473477 (-) 642 WP_062482322.1 SIS domain-containing protein -
  CKY39_RS29835 (CKY39_29835) - 6473563..6473934 (-) 372 WP_095746991.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38132.83 Da        Isoelectric Point: 6.8356

>NTDB_id=245732 CKY39_RS29810 WP_062482334.1 6468828..6469871(+) (pilT) [Variovorax boronicumulans strain J1]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFANALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LTELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=245732 CKY39_RS29810 WP_062482334.1 6468828..6469871(+) (pilT) [Variovorax boronicumulans strain J1]
GTGGACATCACCCAACTGCTGGCGTTCAGCGTCAAGAACAAAGCCTCCGACCTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGAACGGCGACGTGCGCCGCATCAACGTCGATGCGCTGGACCACAAGGCCGTGCATGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTCGCGCGC
TTTCGCGTGAACGCCTTCAACCAGGCGCGCGGCGCGGCCGCGGTGTTCCGGACCATTCCCTCGAAGATCCTCACGCTCGA
GCAGCTCAACGCGCCGAAGATTTTCGGCGAACTCGCGCTCAAGCCGCGCGGCCTCGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTGAACTACCTCAACGAGAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGGCCGATGACGCTGTCGTTTGCCAA
CGCGTTGCGCTCGGCGCTGCGCGAAGACCCGGACGCCATCCTGGTGGGCGAGTTGCGCGACCTCGAAACCATTCGCCTGG
CAATGACCGCGGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAGAAAGAAATGATCCGCGCGATGCTGTCGGAGTCTTTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTCGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCTCGGGCATGCAGACGCTCGACCAGAAC
CTGACAGAGCTGGTGCGCCGCAATACGATCTCTGCCGCCGAAGCGCGCGGCAAGGCCAAGATCCCAGAAAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A554RS02

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Vibrio cholerae strain A1552

68.915

98.271

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.915

98.271

0.677
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Neisseria gonorrhoeae MS11

67.536

99.424

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment