Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CKY39_RS29815 Genome accession   NZ_CP023284
Coordinates   6469901..6471037 (+) Length   378 a.a.
NCBI ID   WP_095746989.1    Uniprot ID   A0A250DRF6
Organism   Variovorax boronicumulans strain J1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6464901..6476037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKY39_RS29785 (CKY39_29785) dapA 6464986..6465879 (+) 894 WP_095746985.1 4-hydroxy-tetrahydrodipicolinate synthase -
  CKY39_RS29790 (CKY39_29790) - 6465886..6466728 (+) 843 WP_095746986.1 alpha/beta hydrolase -
  CKY39_RS29795 (CKY39_29795) - 6466725..6467315 (+) 591 WP_095746987.1 malonic semialdehyde reductase -
  CKY39_RS29800 (CKY39_29800) - 6467326..6468062 (+) 737 Protein_6024 sulfite exporter TauE/SafE family protein -
  CKY39_RS29805 (CKY39_29805) - 6468075..6468776 (-) 702 WP_095746988.1 YggS family pyridoxal phosphate-dependent enzyme -
  CKY39_RS29810 (CKY39_29810) pilT 6468828..6469871 (+) 1044 WP_062482334.1 type IV pilus twitching motility protein PilT Machinery gene
  CKY39_RS29815 (CKY39_29815) pilU 6469901..6471037 (+) 1137 WP_095746989.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CKY39_RS29820 (CKY39_29820) - 6471106..6472014 (+) 909 WP_095746990.1 NAD(P)-dependent oxidoreductase -
  CKY39_RS29825 (CKY39_29825) - 6472045..6472839 (-) 795 WP_062482325.1 BON domain-containing protein -
  CKY39_RS29830 (CKY39_29830) - 6472836..6473477 (-) 642 WP_062482322.1 SIS domain-containing protein -
  CKY39_RS29835 (CKY39_29835) - 6473563..6473934 (-) 372 WP_095746991.1 YraN family protein -
  CKY39_RS29840 (CKY39_29840) rsmI 6473982..6474953 (+) 972 WP_095746992.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  CKY39_RS29845 (CKY39_29845) - 6474957..6475685 (-) 729 WP_095746993.1 glutathione S-transferase -
  CKY39_RS29850 (CKY39_29850) - 6475774..6476001 (-) 228 WP_062481748.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41765.86 Da        Isoelectric Point: 6.3136

>NTDB_id=245733 CKY39_RS29815 WP_095746989.1 6469901..6471037(+) (pilU) [Variovorax boronicumulans strain J1]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAPHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVAMTKRGLTILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGQGRVAAVEILLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFEGNAINFEEAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=245733 CKY39_RS29815 WP_095746989.1 6469901..6471037(+) (pilU) [Variovorax boronicumulans strain J1]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGCGCACCGCACA
CGCTGGCGCTCACGCGCTCGATCATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGACCGGCATCGGGCGCTTTCGCGTGAATGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGATGGCCTGGGCATGCCGCAGGTGCTGAAGGACGTGGCGATGACCAAGCGCGGCCTCACCA
TCCTGGTGGGCGCCACGGGTTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAAAACTCCTACGGC
CACATCGTCACGGTGGAAGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGTGAAGTGGGCAT
CGACACCGACAGCTGGGAGGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCCGTGGCCTTTGCCGAGACCGGTCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCCCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCGCGCAACTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTCTCGCAGCGGCTGGTGCCCACCGAAGACGGCCAGGGCCGCGTGGCCGCCGTCGAGATCCTGCTGAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCACTGTTCGACCTGTTCGAGGGCAACGCGATCAACTTCGAAGAGGCGATCCGCAACGCCGA
TTCGGCCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTGGCGGCCGGCACCGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A250DRF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

57.542

94.709

0.545
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Legionella pneumophila strain ERS1305867

44.681

87.037

0.389
  pilT Legionella pneumophila strain Lp02

44.681

87.037

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37

Multiple sequence alignment