Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSYC_RS01385 Genome accession   NC_007204
Coordinates   321690..322742 (-) Length   350 a.a.
NCBI ID   WP_011279575.1    Uniprot ID   Q4FV21
Organism   Psychrobacter arcticus 273-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 316690..327742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYC_RS01360 (Psyc_0262) - 317468..318610 (+) 1143 WP_011279570.1 hypothetical protein -
  PSYC_RS01365 (Psyc_0263) - 318598..319002 (+) 405 WP_011279571.1 RnfH family protein -
  PSYC_RS01370 (Psyc_0264) - 319028..319444 (-) 417 WP_011279572.1 outer membrane protein assembly factor BamE -
  PSYC_RS01375 (Psyc_0265) fur 319768..320202 (+) 435 WP_011279573.1 ferric iron uptake transcriptional regulator -
  PSYC_RS01380 (Psyc_0266) pilU 320393..321514 (-) 1122 WP_011279574.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSYC_RS01385 (Psyc_0267) pilT 321690..322742 (-) 1053 WP_011279575.1 type IV pilus twitching motility protein PilT Machinery gene
  PSYC_RS01390 (Psyc_0268) - 323058..323813 (+) 756 WP_011279576.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSYC_RS01395 (Psyc_0269) - 324062..325336 (-) 1275 WP_011279577.1 hemolysin family protein -
  PSYC_RS01400 (Psyc_0270) - 325581..327284 (-) 1704 WP_011279578.1 NAD+ synthase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39090.04 Da        Isoelectric Point: 6.5994

>NTDB_id=24429 PSYC_RS01385 WP_011279575.1 321690..322742(-) (pilT) [Psychrobacter arcticus 273-4]
MAEQNLRIEDLLRFAVKLKASDLHISAGMPAMIRVDGEMTRINMPEMSHKVVHQLIYDIMNDRQRADFEEFFETDFSFEI
PDLARFRVNAFNQNRGAGAVFRTIPSKILTMEDLGMGEVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINETRKEHI
LTIEDPIEFVHESKKSLINQREVHRDTLGFEPALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAK
TIDRVIDVFPAAEKDMIRAMLSESLQAVVSQTLLRRQTGGRVAAHEIMLGTPAIRNLIRENKIAQMYSAIQTGAGDGMIT
LDQALKNLVARGTISKDVARPYAKQPEAFL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=24429 PSYC_RS01385 WP_011279575.1 321690..322742(-) (pilT) [Psychrobacter arcticus 273-4]
ATGGCTGAACAAAATTTAAGAATTGAAGACTTGCTGCGTTTTGCCGTCAAGCTTAAGGCTTCAGATTTACATATTTCAGC
CGGTATGCCTGCGATGATTCGAGTCGATGGCGAGATGACTCGGATTAATATGCCAGAGATGAGTCATAAAGTGGTGCATC
AACTCATTTATGACATCATGAATGATAGACAACGTGCCGACTTTGAAGAATTTTTTGAGACGGATTTTTCTTTTGAGATT
CCAGATTTGGCCCGTTTTCGGGTAAACGCATTTAATCAAAATCGCGGTGCAGGCGCTGTCTTTCGTACGATTCCATCCAA
AATTCTGACCATGGAAGATCTAGGTATGGGAGAAGTTTTTAAGCGTGTCTCAGACTTTAAGCGTGGCGTGGTATTGGTCA
CTGGTCCAACCGGCTCAGGTAAGTCGACCACACTGGCGGCCATGATTGATTATATCAATGAAACCCGTAAAGAGCATATC
TTGACCATTGAAGACCCGATTGAATTTGTTCATGAGTCAAAAAAGAGCTTAATTAACCAGCGTGAAGTACACCGCGACAC
CTTAGGTTTCGAACCGGCATTGCGCTCAGCTTTACGTGAAGATCCTGATATTATTCTAGTTGGTGAGCTACGTGACCTTG
AGACCATACGCTTAGCGCTCACTGCCGCTGAAACCGGACACTTAGTCTTTGGAACTTTACACACCAGTTCCGCTGCCAAA
ACCATTGACCGTGTGATCGACGTATTCCCTGCTGCTGAAAAAGACATGATTCGAGCAATGCTATCAGAGTCACTACAAGC
AGTGGTCTCGCAAACGCTTTTACGTCGTCAAACAGGCGGGCGAGTTGCCGCGCATGAGATTATGCTGGGTACGCCCGCTA
TTCGTAACTTGATACGTGAAAACAAAATTGCTCAAATGTATTCTGCTATCCAAACGGGTGCTGGTGATGGCATGATTACT
CTCGATCAAGCCCTTAAAAATCTTGTAGCGAGAGGCACCATTAGCAAAGACGTTGCACGACCTTATGCTAAGCAGCCTGA
AGCCTTTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4FV21

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

79.883

98

0.783
  pilT Acinetobacter baumannii D1279779

79.883

98

0.783
  pilT Acinetobacter nosocomialis M2

79.592

98

0.78
  pilT Pseudomonas aeruginosa PAK

78.655

97.714

0.769
  pilT Pseudomonas stutzeri DSM 10701

78.07

97.714

0.763
  pilT Acinetobacter baylyi ADP1

76.023

97.714

0.743
  pilT Legionella pneumophila strain Lp02

73.099

97.714

0.714
  pilT Legionella pneumophila strain ERS1305867

73.099

97.714

0.714
  pilT Neisseria meningitidis 8013

64.058

98.571

0.631
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.584

93.429

0.631
  pilT Vibrio cholerae strain A1552

67.584

93.429

0.631
  pilT Neisseria gonorrhoeae MS11

63.478

98.571

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.187

98

0.511
  pilU Vibrio cholerae strain A1552

43.789

92

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.593

96.857

0.403
  pilU Acinetobacter baylyi ADP1

41.593

96.857

0.403

Multiple sequence alignment