Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PSYC_RS01380 Genome accession   NC_007204
Coordinates   320393..321514 (-) Length   373 a.a.
NCBI ID   WP_011279574.1    Uniprot ID   Q4FV22
Organism   Psychrobacter arcticus 273-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 315393..326514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYC_RS01350 (Psyc_0260) - 315850..316227 (+) 378 WP_406621526.1 c-type cytochrome -
  PSYC_RS01355 (Psyc_0261) - 316570..317238 (+) 669 WP_011279569.1 c-type cytochrome -
  PSYC_RS01360 (Psyc_0262) - 317468..318610 (+) 1143 WP_011279570.1 hypothetical protein -
  PSYC_RS01365 (Psyc_0263) - 318598..319002 (+) 405 WP_011279571.1 RnfH family protein -
  PSYC_RS01370 (Psyc_0264) - 319028..319444 (-) 417 WP_011279572.1 outer membrane protein assembly factor BamE -
  PSYC_RS01375 (Psyc_0265) fur 319768..320202 (+) 435 WP_011279573.1 ferric iron uptake transcriptional regulator -
  PSYC_RS01380 (Psyc_0266) pilU 320393..321514 (-) 1122 WP_011279574.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSYC_RS01385 (Psyc_0267) pilT 321690..322742 (-) 1053 WP_011279575.1 type IV pilus twitching motility protein PilT Machinery gene
  PSYC_RS01390 (Psyc_0268) - 323058..323813 (+) 756 WP_011279576.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSYC_RS01395 (Psyc_0269) - 324062..325336 (-) 1275 WP_011279577.1 hemolysin family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41591.86 Da        Isoelectric Point: 6.7847

>NTDB_id=24428 PSYC_RS01380 WP_011279574.1 320393..321514(-) (pilU) [Psychrobacter arcticus 273-4]
MDIDKLLQLMINKNGSDLFITADVAPSMKINGKILPVGKIPLTAEQTMRLVKGVMTVNQQKEFDETHECQFAITDQAQQA
RFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELAMKNHGLVLLVGATGTGKSTTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFETGLKNTLRQAPDVILIGEIRNLEVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFEASRHNQLFMDLSLNLQAIIAQKLIPTPDGKGRRAAIEILLNSQLISDHIRKGDVHEIKDVMTRSRDSGMQTFDQ
ALFDLYENGEITYKEAILHADSPNDLRLKIKLSSKNGVANLDEGADSLSLDIT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=24428 PSYC_RS01380 WP_011279574.1 320393..321514(-) (pilU) [Psychrobacter arcticus 273-4]
ATGGACATTGATAAACTTTTACAATTGATGATTAATAAAAATGGCTCCGACCTTTTTATCACTGCCGATGTCGCACCTTC
TATGAAAATCAATGGGAAAATACTGCCTGTTGGTAAAATACCTTTGACCGCTGAGCAAACCATGAGGCTGGTTAAAGGTG
TCATGACAGTCAATCAGCAAAAAGAATTTGATGAGACCCATGAGTGTCAGTTTGCGATTACTGATCAGGCGCAACAAGCA
CGTTTTCGGGTTAGTGCTTTTATACAGCGCGACATGGCAGGGATGATTTTAAGGAAAATCGAAAACAAAATCCCTACTGT
CGAAGAGCTACGTCTGCCGCCAGCGCTAAAAGAGCTGGCAATGAAAAATCACGGTTTGGTTTTATTAGTTGGCGCAACCG
GTACTGGTAAATCAACCACGCTTGCAGCCATGATAGGACATCGTAATCAAAATTCTCATGGTCATATTATTACCATAGAA
GACCCTATCGAGTTTGTCCATAAACATCGCGGCTGTATTATTACCCAGCGCGAAGTCGGTATCGACACCCACTCTTTTGA
AACCGGTCTAAAAAATACCTTGCGCCAAGCACCTGACGTTATTTTGATTGGTGAGATTCGTAATCTCGAAGTCATGAGTT
ACGCGATTCAATATGCTGAAACGGGGCATTTGGTATTTGCAACCTTGCACGCCAATAATGCTAACCAAGCAATCGACCGT
ATTATTCACTTTTTTGAGGCCAGTCGCCATAATCAATTGTTTATGGATTTGTCGCTTAACTTGCAGGCCATTATCGCGCA
GAAATTGATTCCAACGCCTGATGGCAAGGGACGCCGCGCCGCTATTGAAATATTGTTAAACTCACAGCTGATTAGCGATC
ATATCCGTAAAGGTGATGTGCATGAGATCAAAGATGTCATGACACGCTCACGTGATAGCGGTATGCAAACCTTTGACCAA
GCTTTGTTTGATTTATATGAAAATGGTGAAATTACTTATAAAGAAGCTATTCTTCATGCTGATTCTCCCAATGATTTGCG
CTTAAAAATCAAACTCAGCAGCAAAAATGGCGTGGCAAACTTAGATGAAGGCGCAGATAGTTTATCGTTAGACATTACCT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4FV22

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.416

100

0.654
  pilU Acinetobacter baylyi ADP1

65.07

95.174

0.619
  pilU Vibrio cholerae strain A1552

51.359

98.66

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.803

98.123

0.41
  pilT Vibrio cholerae strain A1552

42.09

89.812

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.09

89.812

0.378
  pilT Acinetobacter nosocomialis M2

39.884

92.761

0.37
  pilT Acinetobacter baumannii D1279779

39.884

92.761

0.37
  pilT Acinetobacter baumannii strain A118

39.884

92.761

0.37
  pilT Pseudomonas aeruginosa PAK

39.884

92.761

0.37
  pilT Legionella pneumophila strain Lp02

42.154

87.131

0.367
  pilT Legionella pneumophila strain ERS1305867

42.154

87.131

0.367
  pilT Acinetobacter baylyi ADP1

39.017

92.761

0.362

Multiple sequence alignment