Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CJ010_RS21155 Genome accession   NZ_CP022958
Coordinates   4565368..4566408 (+) Length   346 a.a.
NCBI ID   WP_141020802.1    Uniprot ID   A0A4Y6KY39
Organism   Azoarcus sp. DD4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4560368..4571408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJ010_RS21130 (CJ010_21070) - 4561397..4562680 (+) 1284 WP_141019877.1 dihydroorotase -
  CJ010_RS21135 (CJ010_21075) - 4562703..4562999 (-) 297 WP_141019878.1 DUF167 domain-containing protein -
  CJ010_RS21140 (CJ010_21080) - 4563002..4563583 (-) 582 WP_141019879.1 YggT family protein -
  CJ010_RS21145 (CJ010_21085) proC 4563602..4564426 (-) 825 WP_141019880.1 pyrroline-5-carboxylate reductase -
  CJ010_RS21150 (CJ010_21090) - 4564521..4565234 (-) 714 WP_141019881.1 YggS family pyridoxal phosphate-dependent enzyme -
  CJ010_RS21155 (CJ010_21095) pilT 4565368..4566408 (+) 1041 WP_141020802.1 type IV pilus twitching motility protein PilT Machinery gene
  CJ010_RS21160 (CJ010_21100) pilU 4566513..4567646 (+) 1134 WP_141019882.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CJ010_RS25755 - 4567695..4568195 (-) 501 WP_371415716.1 CZB domain-containing protein -
  CJ010_RS25760 - 4568169..4568858 (-) 690 Protein_4180 methyl-accepting chemotaxis protein -
  CJ010_RS25765 - 4568901..4569170 (-) 270 Protein_4181 hypothetical protein -
  CJ010_RS21170 (CJ010_21110) - 4569145..4569666 (-) 522 WP_141019884.1 PAS domain-containing protein -
  CJ010_RS21175 (CJ010_21115) - 4569713..4570879 (-) 1167 WP_141019885.1 class I SAM-dependent RNA methyltransferase -
  CJ010_RS21180 (CJ010_21120) - 4571138..4571356 (-) 219 WP_141019886.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38517.10 Da        Isoelectric Point: 7.0158

>NTDB_id=243728 CJ010_RS21155 WP_141020802.1 4565368..4566408(+) (pilT) [Azoarcus sp. DD4]
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKDVHAMVYDIMNDGQRKQFEETWECDFSFAVPNLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEELNCPKIFKDISNQPRGIVLVTGPTGSGKSTTLAAMIDYINENEYGHILTVED
PIEFVHESKRCLINQREVHRDTASFNNALRASLREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGSGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQNVGMQTLDQC
LADLVRRNIVSAAEARVRAQNKDNFN

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=243728 CJ010_RS21155 WP_141020802.1 4565368..4566408(+) (pilT) [Azoarcus sp. DD4]
ATGGACATTACCGAACTGCTCGCCTTCGCGGTCAAGAACAAGGCCTCCGACCTTCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGCATGGCGACGTGCGCCGCATCAACCTGCCGCCCATGGAGCACAAGGACGTGCACGCCATGGTGTACGACA
TCATGAACGACGGGCAGCGCAAGCAGTTCGAGGAAACCTGGGAGTGCGACTTCTCGTTCGCGGTGCCCAACCTGGCCCGC
TTCCGGGTCAATGCCTTCAACCAGAACCGCGGCGCGGGCGCGGTATTCCGGACGATTCCGTCCAAGGTGCTGACGCTCGA
AGAGCTCAACTGCCCGAAGATCTTCAAAGACATCTCGAATCAGCCGCGCGGCATCGTGCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACGAGAACGAGTACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACGAATCCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCGCGTCCTTCAACAA
TGCGCTGCGCGCCTCGCTGCGCGAGGATCCGGACGTTATCCTGGTCGGCGAAATGCGCGACCTCGAAACCATCCGCCTGG
CGCTGACCGCAGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATAGACCGTATC
GTCGACGTCTTCCCCGCGGCGGAAAAGGACATGGTGCGGTCGATGCTGTCGGAATCCCTGCGCGCAGTCATCTCGCAGAC
CCTGCTCAAGACCAAGGACGGCTCCGGCCGGGTCGCGGCGCACGAGATCATGATAGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAAAACAAGATCGCGCAGATGTACTCCGCCATCCAGACCGGCCAGAACGTCGGCATGCAGACACTGGACCAATGC
CTGGCCGATCTCGTCCGCCGCAACATCGTCTCGGCGGCAGAAGCGCGCGTGCGGGCGCAGAACAAGGACAACTTCAACTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y6KY39

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80

99.711

0.798
  pilT Acinetobacter baumannii strain A118

80

99.711

0.798
  pilT Acinetobacter nosocomialis M2

80

99.711

0.798
  pilT Pseudomonas aeruginosa PAK

77.971

99.711

0.777
  pilT Acinetobacter baylyi ADP1

77.101

99.711

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.522

99.711

0.763
  pilT Legionella pneumophila strain Lp02

73.333

99.711

0.731
  pilT Legionella pneumophila strain ERS1305867

73.333

99.711

0.731
  pilT Neisseria meningitidis 8013

70.809

100

0.708
  pilT Neisseria gonorrhoeae MS11

70.52

100

0.705
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.647

98.266

0.665
  pilT Vibrio cholerae strain A1552

67.647

98.266

0.665
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.623

99.711

0.535
  pilU Acinetobacter baylyi ADP1

41.954

100

0.422
  pilU Vibrio cholerae strain A1552

42.985

96.821

0.416
  pilU Pseudomonas stutzeri DSM 10701

41.159

99.711

0.41

Multiple sequence alignment