Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CJ010_RS21160 Genome accession   NZ_CP022958
Coordinates   4566513..4567646 (+) Length   377 a.a.
NCBI ID   WP_141019882.1    Uniprot ID   -
Organism   Azoarcus sp. DD4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4561513..4572646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJ010_RS21135 (CJ010_21075) - 4562703..4562999 (-) 297 WP_141019878.1 DUF167 domain-containing protein -
  CJ010_RS21140 (CJ010_21080) - 4563002..4563583 (-) 582 WP_141019879.1 YggT family protein -
  CJ010_RS21145 (CJ010_21085) proC 4563602..4564426 (-) 825 WP_141019880.1 pyrroline-5-carboxylate reductase -
  CJ010_RS21150 (CJ010_21090) - 4564521..4565234 (-) 714 WP_141019881.1 YggS family pyridoxal phosphate-dependent enzyme -
  CJ010_RS21155 (CJ010_21095) pilT 4565368..4566408 (+) 1041 WP_141020802.1 type IV pilus twitching motility protein PilT Machinery gene
  CJ010_RS21160 (CJ010_21100) pilU 4566513..4567646 (+) 1134 WP_141019882.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CJ010_RS25755 - 4567695..4568195 (-) 501 WP_371415716.1 CZB domain-containing protein -
  CJ010_RS25760 - 4568169..4568858 (-) 690 Protein_4180 methyl-accepting chemotaxis protein -
  CJ010_RS25765 - 4568901..4569170 (-) 270 Protein_4181 hypothetical protein -
  CJ010_RS21170 (CJ010_21110) - 4569145..4569666 (-) 522 WP_141019884.1 PAS domain-containing protein -
  CJ010_RS21175 (CJ010_21115) - 4569713..4570879 (-) 1167 WP_141019885.1 class I SAM-dependent RNA methyltransferase -
  CJ010_RS21180 (CJ010_21120) - 4571138..4571356 (-) 219 WP_141019886.1 hypothetical protein -
  CJ010_RS21185 (CJ010_21125) - 4571353..4572387 (-) 1035 WP_141019887.1 formate dehydrogenase subunit gamma -
  CJ010_RS21190 (CJ010_21130) - 4572398..4572625 (-) 228 WP_141019888.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42114.52 Da        Isoelectric Point: 6.4000

>NTDB_id=243729 CJ010_RS21160 WP_141019882.1 4566513..4567646(+) (pilU) [Azoarcus sp. DD4]
MERDQALKFMHDLLRLMLQKNGSDLFINAGFPPAIKIDGRITPQSNQALAPPHTAELARAIMNDRQSAEFEATKECNFAI
SPAGIGRFRANAYIQQGRVALVLRTIPQKIPTLDELGLPPVLRDIGMTKRGLVIFVGGTGTGKTTSLAAMVDYRNENSYG
HIITVEDPIEFVHPHKNCIVSQREIGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRQQLLMDLSLNLRAMVSQRLLPLKGRKGRVPAVEVMLNSPLISDLIFKGEVPGIKEIMKRSRELG
MQTFDQALFDLYEEEKISYEDALRNADSVNDLRLQIKLNSKLGDKDLVSGVQNLDIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=243729 CJ010_RS21160 WP_141019882.1 4566513..4567646(+) (pilU) [Azoarcus sp. DD4]
ATGGAACGCGATCAGGCCCTCAAGTTCATGCACGATCTGCTGCGGCTGATGCTGCAGAAGAACGGCTCCGACCTCTTCAT
CAATGCCGGCTTCCCGCCGGCGATAAAGATCGACGGCCGCATCACGCCGCAGTCCAATCAGGCACTGGCGCCGCCGCACA
CCGCCGAGCTCGCACGCGCGATCATGAACGACCGCCAGTCGGCCGAGTTCGAAGCCACCAAGGAGTGCAACTTCGCGATT
TCGCCGGCGGGCATCGGCCGCTTCCGCGCCAACGCCTACATCCAGCAGGGGCGTGTGGCGCTGGTGCTGCGGACCATCCC
ACAGAAGATACCCACCCTCGACGAACTCGGCCTGCCGCCGGTCCTGCGCGACATCGGCATGACCAAGCGCGGCCTGGTCA
TCTTCGTCGGCGGCACCGGCACCGGCAAGACCACCTCGCTGGCGGCGATGGTCGACTATCGCAACGAGAACTCCTACGGC
CACATCATCACGGTCGAAGACCCAATCGAATTCGTCCATCCGCACAAGAACTGCATCGTGTCGCAGCGTGAGATCGGCAT
CGACACCGACAGCTGGGAAGCCGCACTGAAGAACACCCTGCGGCAGGCGCCGGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAAACGATGGACTACGCGATCGCCTTCGCCGAAACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAGCGCC
AACCAGGCGATCGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCCAGCAGCTGCTGATGGATCTCTCGCTCAACCT
GCGGGCGATGGTGTCGCAGCGCTTGCTGCCGTTGAAGGGACGCAAGGGCCGGGTGCCGGCGGTGGAGGTGATGCTCAACT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTGCCGGGCATCAAGGAGATCATGAAGCGCTCGCGCGAGCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTCTACGAGGAAGAGAAGATCAGCTACGAGGATGCGTTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCCTGCAGATCAAGCTCAACAGCAAGCTCGGCGACAAGGATCTCGTCTCGGGCGTGCAGAACC
TCGACATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(127-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.088

96.552

0.599
  pilU Acinetobacter baylyi ADP1

59.544

93.103

0.554
  pilU Vibrio cholerae strain A1552

53.889

95.491

0.515
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.458

94.96

0.403
  pilT Legionella pneumophila strain Lp02

43.505

87.798

0.382
  pilT Legionella pneumophila strain ERS1305867

43.505

87.798

0.382
  pilT Pseudomonas stutzeri DSM 10701

42.09

88.859

0.374
  pilT Acinetobacter baumannii D1279779

42.598

87.798

0.374
  pilT Acinetobacter nosocomialis M2

42.598

87.798

0.374
  pilT Acinetobacter baumannii strain A118

42.598

87.798

0.374
  pilT Acinetobacter baylyi ADP1

43.75

84.881

0.371
  pilT Pseudomonas aeruginosa PAK

41.791

88.859

0.371
  pilT Vibrio cholerae strain A1552

43.492

83.554

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.492

83.554

0.363

Multiple sequence alignment