Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Kalk_RS12930 Genome accession   NZ_CP022684
Coordinates   3022133..3023170 (+) Length   345 a.a.
NCBI ID   WP_101894645.1    Uniprot ID   A0A2K9LM11
Organism   Ketobacter alkanivorans strain GI5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3017133..3028170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Kalk_RS12900 (Kalk_12885) - 3017368..3017661 (-) 294 WP_101894639.1 DUF167 domain-containing protein -
  Kalk_RS12905 (Kalk_12890) - 3017665..3018225 (-) 561 WP_101894640.1 YggT family protein -
  Kalk_RS12910 (Kalk_12895) proC 3018349..3019158 (-) 810 WP_101894641.1 pyrroline-5-carboxylate reductase -
  Kalk_RS12915 (Kalk_12900) - 3019282..3019947 (-) 666 WP_101894642.1 GntR family transcriptional regulator -
  Kalk_RS12920 (Kalk_12905) - 3020083..3021243 (+) 1161 WP_101894643.1 alkane 1-monooxygenase -
  Kalk_RS12925 (Kalk_12910) - 3021256..3021948 (-) 693 WP_101894644.1 YggS family pyridoxal phosphate-dependent enzyme -
  Kalk_RS12930 (Kalk_12915) pilT 3022133..3023170 (+) 1038 WP_101894645.1 type IV pilus twitching motility protein PilT Machinery gene
  Kalk_RS12935 (Kalk_12920) pilU 3023201..3024340 (+) 1140 WP_101894646.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Kalk_RS12940 (Kalk_12925) - 3024390..3024983 (+) 594 WP_101894647.1 DUF6436 domain-containing protein -
  Kalk_RS12945 (Kalk_12930) - 3025006..3026310 (-) 1305 WP_101894648.1 dihydroorotase -
  Kalk_RS12950 (Kalk_12935) - 3026310..3027308 (-) 999 WP_101894649.1 aspartate carbamoyltransferase catalytic subunit -
  Kalk_RS12955 (Kalk_12940) pyrR 3027305..3027811 (-) 507 WP_101894650.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37993.69 Da        Isoelectric Point: 6.3474

>NTDB_id=241649 Kalk_RS12930 WP_101894645.1 3022133..3023170(+) (pilT) [Ketobacter alkanivorans strain GI5]
MDITELLAFGVKNGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHGLIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGQGQVFKDICEFPRGLVLVTGPTGSGKSTTLAAMLDYVNETRYEHILTIED
PIEFVHESKKCLLNQREVHRDTLGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAAEKAMVRSMLSESLQAVVSQTLLKKTGGGRVAAHEIMIGTPAIRNLIREDKIAQMYSAIQTGASLGMQTLDQCL
GDLVRKGHVSIELAREKAKNPDGLK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=241649 Kalk_RS12930 WP_101894645.1 3022133..3023170(+) (pilT) [Ketobacter alkanivorans strain GI5]
ATGGATATTACGGAGTTGCTTGCCTTTGGCGTGAAAAACGGTGCGTCTGACTTACACCTGTCGGCCGGTTTGCCCCCCAT
GATCCGGGTTGATGGCGATGTTCGCCGAATCAACCTGCCAGCAATGGATCATAAACAGGTGCATGGCCTCATTTATGACA
TCATGAATGATAAGCAGCGTAAGGATTTCGAAGAATTTCTGGAAACGGATTTCTCTTTCGAGGTGCCGGGTGTGGCTCGC
TTCCGGGTTAACGCCTTTAACCAGAACCGGGGCGCCGGTGCGGTGTTCCGAACCATTCCCTCCAAAGTGTTATCCATGGA
GGATCTGGGCCAGGGGCAAGTGTTCAAAGATATCTGTGAGTTTCCCCGTGGCTTGGTATTGGTAACCGGGCCTACCGGCT
CCGGCAAATCCACAACCCTGGCGGCGATGCTGGATTACGTTAACGAAACCCGTTACGAACACATCCTCACCATCGAAGAC
CCCATCGAATTCGTACACGAATCCAAAAAATGTCTACTTAACCAACGGGAAGTGCATCGTGATACCCTCGGTTTTGCGGA
GGCCCTGCGTTCAGCGCTGCGGGAAGATCCCGATATCATCCTGGTGGGTGAGATGCGAGATCTGGAAACCATTCGTTTGG
CGCTGACTGCCGCTGAAACCGGTCACTTGGTGTTCGGCACCCTCCACACCACCTCTGCGGCCAAAACCATTGACCGTGTG
GTGGATGTGTTCCCGGCAGCGGAAAAAGCCATGGTGCGCTCCATGTTGTCGGAATCCCTGCAGGCAGTTGTGTCCCAGAC
ACTGTTGAAAAAAACCGGTGGTGGCCGGGTGGCGGCCCATGAAATTATGATTGGCACACCGGCTATTCGAAACCTGATTC
GTGAAGATAAAATTGCCCAGATGTACTCGGCCATTCAAACCGGAGCATCATTGGGGATGCAGACCCTGGATCAATGTCTG
GGTGATTTGGTGCGCAAGGGCCATGTTAGTATCGAGCTGGCTCGCGAAAAGGCCAAGAACCCCGACGGACTTAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9LM11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

99.71

0.878
  pilT Pseudomonas aeruginosa PAK

88.081

99.71

0.878
  pilT Acinetobacter nosocomialis M2

84.302

99.71

0.841
  pilT Acinetobacter baumannii D1279779

84.302

99.71

0.841
  pilT Acinetobacter baumannii strain A118

84.302

99.71

0.841
  pilT Acinetobacter baylyi ADP1

81.686

99.71

0.814
  pilT Legionella pneumophila strain Lp02

75.882

98.551

0.748
  pilT Legionella pneumophila strain ERS1305867

75.882

98.551

0.748
  pilT Neisseria meningitidis 8013

68.986

100

0.69
  pilT Neisseria gonorrhoeae MS11

68.696

100

0.687
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.681

0.664
  pilT Vibrio cholerae strain A1552

67.953

97.681

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.13

0.513
  pilU Pseudomonas stutzeri DSM 10701

40.462

100

0.406
  pilU Acinetobacter baylyi ADP1

39.769

100

0.4
  pilU Vibrio cholerae strain A1552

39.306

100

0.394

Multiple sequence alignment