Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Kalk_RS12935 Genome accession   NZ_CP022684
Coordinates   3023201..3024340 (+) Length   379 a.a.
NCBI ID   WP_101894646.1    Uniprot ID   A0A2K9LLR2
Organism   Ketobacter alkanivorans strain GI5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3018201..3029340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Kalk_RS12910 (Kalk_12895) proC 3018349..3019158 (-) 810 WP_101894641.1 pyrroline-5-carboxylate reductase -
  Kalk_RS12915 (Kalk_12900) - 3019282..3019947 (-) 666 WP_101894642.1 GntR family transcriptional regulator -
  Kalk_RS12920 (Kalk_12905) - 3020083..3021243 (+) 1161 WP_101894643.1 alkane 1-monooxygenase -
  Kalk_RS12925 (Kalk_12910) - 3021256..3021948 (-) 693 WP_101894644.1 YggS family pyridoxal phosphate-dependent enzyme -
  Kalk_RS12930 (Kalk_12915) pilT 3022133..3023170 (+) 1038 WP_101894645.1 type IV pilus twitching motility protein PilT Machinery gene
  Kalk_RS12935 (Kalk_12920) pilU 3023201..3024340 (+) 1140 WP_101894646.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Kalk_RS12940 (Kalk_12925) - 3024390..3024983 (+) 594 WP_101894647.1 DUF6436 domain-containing protein -
  Kalk_RS12945 (Kalk_12930) - 3025006..3026310 (-) 1305 WP_101894648.1 dihydroorotase -
  Kalk_RS12950 (Kalk_12935) - 3026310..3027308 (-) 999 WP_101894649.1 aspartate carbamoyltransferase catalytic subunit -
  Kalk_RS12955 (Kalk_12940) pyrR 3027305..3027811 (-) 507 WP_101894650.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  Kalk_RS12960 (Kalk_12945) ruvX 3027811..3028263 (-) 453 WP_101894651.1 Holliday junction resolvase RuvX -
  Kalk_RS12965 (Kalk_12950) - 3028285..3028857 (-) 573 WP_101894652.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42032.32 Da        Isoelectric Point: 6.6403

>NTDB_id=241650 Kalk_RS12935 WP_101894646.1 3023201..3024340(+) (pilU) [Ketobacter alkanivorans strain GI5]
MDFEDLLKLMVEKGGSDLFITAGVPPSMKVNGRVVPVTKTALSPEKTRETVLGVMNEGQRKEFIESHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETRIPTIDELNLPSVIKDLAMTKRGIVIFVGATGTGKSTSLASMIGHRNNNSKGHIISIED
PIEYIHQHKGCIVTQREVGLDTESFDIALKNTLRQAPDVIMIGEIRTRETMEYAVAFSETGHLVLATLHANNANQALDRI
IHFFPADRHNQLWMDLSLNLRAMVAQQLIPTPDGKSRRAVIEVLLNTPLMADHIRKGEVHLLKPLMAKSRELGMQTFDQA
LYDLYSAGEITYEDALAHADSPNDLRLMIKLGSESSADALDSATKGLTLQDDDGMKTGW

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=241650 Kalk_RS12935 WP_101894646.1 3023201..3024340(+) (pilU) [Ketobacter alkanivorans strain GI5]
ATGGATTTTGAAGACCTGCTAAAGCTGATGGTAGAAAAAGGAGGATCTGACCTGTTTATAACGGCGGGCGTCCCGCCTTC
CATGAAAGTGAACGGCCGGGTTGTGCCCGTTACCAAAACCGCGTTGAGCCCCGAAAAAACCCGCGAGACGGTGCTGGGTG
TCATGAACGAAGGCCAGCGTAAGGAATTTATCGAATCCCATGAATGTAACTTCGCCATATCGGCGCGGGGCATAGGTCGT
TTCCGGGTCAGCGCGTTTTATCAGCGCAACCTGGTGGGCATGGTGTTGCGTCGCATCGAAACCCGTATCCCCACCATCGA
TGAGCTGAATCTACCGTCGGTCATCAAAGATCTGGCCATGACCAAGCGCGGCATCGTGATTTTTGTAGGGGCGACCGGTA
CCGGTAAATCCACCTCGCTGGCCTCCATGATTGGCCATCGCAACAATAACTCCAAGGGTCATATCATCAGCATCGAAGAC
CCTATTGAATACATCCACCAACACAAGGGCTGCATTGTTACCCAGCGGGAAGTGGGCCTGGATACGGAAAGCTTTGATAT
AGCCCTGAAGAACACCCTGCGACAGGCACCGGATGTCATCATGATCGGTGAGATTCGAACCCGCGAGACCATGGAATACG
CTGTGGCGTTCTCCGAGACCGGTCACCTGGTGCTGGCTACTCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGTATC
ATTCACTTCTTCCCGGCGGATCGTCACAACCAGTTGTGGATGGACTTGTCGCTCAATTTACGCGCCATGGTGGCGCAACA
ATTGATCCCGACCCCCGATGGCAAAAGTCGCCGCGCGGTTATTGAAGTGTTGCTCAACACCCCATTGATGGCGGATCACA
TCCGCAAGGGTGAAGTGCACCTGCTGAAACCGTTGATGGCCAAGTCCCGCGAGCTGGGCATGCAGACCTTCGATCAGGCG
CTCTACGATCTGTACAGTGCCGGTGAGATCACCTACGAAGACGCGCTGGCCCACGCCGATTCGCCCAACGACCTGCGTTT
GATGATCAAACTTGGCTCGGAAAGCTCGGCTGATGCGCTGGACAGCGCAACCAAAGGGCTGACCTTGCAAGATGATGATG
GCATGAAAACCGGTTGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9LLR2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

79.733

98.945

0.789
  pilU Acinetobacter baylyi ADP1

68.067

94.195

0.641
  pilU Vibrio cholerae strain A1552

56.857

92.348

0.525
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.137

94.195

0.406

Multiple sequence alignment