Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CG015_RS03160 Genome accession   NZ_CP022468
Coordinates   651105..652142 (+) Length   345 a.a.
NCBI ID   WP_013857819.1    Uniprot ID   -
Organism   Vibrio anguillarum strain MHK3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 646105..657142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG015_RS03125 (CG015_03110) hemW 646345..647502 (-) 1158 WP_129532220.1 radical SAM family heme chaperone HemW -
  CG015_RS03130 (CG015_03115) - 647502..648101 (-) 600 WP_017042394.1 XTP/dITP diphosphatase -
  CG015_RS03135 (CG015_03120) - 648114..648545 (-) 432 WP_010320641.1 DUF4426 domain-containing protein -
  CG015_RS03140 (CG015_03125) yggU 648588..648878 (-) 291 WP_129532221.1 DUF167 family protein YggU -
  CG015_RS03145 (CG015_03130) - 648878..649435 (-) 558 WP_010320643.1 YggT family protein -
  CG015_RS03150 (CG015_03135) proC 649498..650316 (-) 819 WP_064624328.1 pyrroline-5-carboxylate reductase -
  CG015_RS03155 (CG015_03140) - 650378..651079 (-) 702 WP_017048292.1 YggS family pyridoxal phosphate-dependent enzyme -
  CG015_RS03160 (CG015_03145) pilT 651105..652142 (+) 1038 WP_013857819.1 type IV pilus twitching motility protein PilT Machinery gene
  CG015_RS03165 (CG015_03150) pilU 652153..653259 (+) 1107 WP_129532222.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CG015_RS03170 (CG015_03155) ruvX 653343..653771 (-) 429 WP_017045508.1 Holliday junction resolvase RuvX -
  CG015_RS03175 (CG015_03160) - 653854..654417 (-) 564 WP_129532223.1 YqgE/AlgH family protein -
  CG015_RS03180 (CG015_03165) gshB 654464..655417 (-) 954 WP_129532224.1 glutathione synthase -
  CG015_RS03185 (CG015_03170) rsmE 655431..656162 (-) 732 WP_013857814.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CG015_RS03190 (CG015_03175) endA 656271..656978 (-) 708 WP_013857813.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38275.79 Da        Isoelectric Point: 6.4491

>NTDB_id=239706 CG015_RS03160 WP_013857819.1 651105..652142(+) (pilT) [Vibrio anguillarum strain MHK3]
MDIAELLDFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHADVHRLIFEIMNDAQRSEFEEKLEVDFSFELPNVGR
FRVNAFHQSRGCSAVFRTIPTVIPTLEELQAPDIFNKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINSHHNKHILTIED
PIEFVHSNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKEMVRSMLSESLRAVIAQKLLKRLGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNS
KQLIAQGIVDQEEVQKKLEVNSSPF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=239706 CG015_RS03160 WP_013857819.1 651105..652142(+) (pilT) [Vibrio anguillarum strain MHK3]
ATGGATATCGCTGAGCTACTGGATTTTAGTGTAAAGCACAACGCTTCGGATCTACACCTATCTGCAGGTGTTCCACCTAT
GGTACGGATAGATGGTGAAGTAAGAAAGCTCGGAGTGCCTGCTTTTACTCATGCTGACGTGCATCGTTTGATTTTTGAGA
TCATGAACGATGCTCAGCGTAGTGAATTTGAAGAGAAACTGGAAGTGGATTTCTCATTTGAGCTGCCTAATGTAGGGCGT
TTTCGTGTCAATGCTTTCCATCAATCTCGTGGCTGCTCTGCGGTTTTCCGGACGATTCCTACGGTGATCCCAACCTTGGA
AGAACTACAAGCGCCTGACATTTTTAATAAAATCGCCAATTATGAGAAAGGATTGGTGCTGGTGACTGGGCCAACAGGGT
CGGGTAAATCAACCACACTGGCAGCAATGGTTGATTACATTAATTCTCATCACAACAAACACATTTTAACCATTGAAGAC
CCCATTGAGTTTGTGCATAGCAACAATAAATGTTTGGTTAACCAACGTGAAGTGCACCGAGATACCCACAGTTTTAAAGC
GGCGCTACGCTCGGCGCTGCGCGAAGATCCGGATGTAATCTTGGTCGGTGAGCTGCGCGATCAAGAAACCATCAGCTTAG
CGTTAACCGCGGCTGAAACCGGGCATTTAGTGTTCGGCACGTTACATACCAGCTCTGCGGCGAAAACCATCGACCGGATC
ATTGATGTGTTTCCCGGTAGTGATAAAGAGATGGTTCGTTCAATGCTCTCTGAATCATTACGAGCCGTGATTGCGCAAAA
GTTGCTAAAACGTTTAGGTGGGGGCCGCGTCGCATGTCATGAAATTATGCTTGCCACTCCAGCGATTCGTAACTTGATCC
GCGAGGATAAGGTTGCGCAGATGTATTCCATCATCCAAACGGGTGCGGCACATGGCATGCAAACCATGGAACAGAATTCC
AAACAGTTGATTGCGCAAGGTATTGTCGATCAAGAAGAAGTGCAGAAAAAACTGGAAGTCAATTCATCACCATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

92.174

100

0.922
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

92.174

100

0.922
  pilT Acinetobacter baylyi ADP1

72.424

95.652

0.693
  pilT Acinetobacter baumannii D1279779

72.036

95.362

0.687
  pilT Acinetobacter baumannii strain A118

72.036

95.362

0.687
  pilT Acinetobacter nosocomialis M2

71.733

95.362

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Pseudomonas aeruginosa PAK

67.941

98.551

0.67
  pilT Legionella pneumophila strain Lp02

68.882

95.942

0.661
  pilT Legionella pneumophila strain ERS1305867

68.882

95.942

0.661
  pilT Neisseria meningitidis 8013

62.5

99.71

0.623
  pilT Neisseria gonorrhoeae MS11

62.209

99.71

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.774

92.174

0.496
  pilU Vibrio cholerae strain A1552

43.413

96.812

0.42
  pilU Pseudomonas stutzeri DSM 10701

42.308

97.971

0.414
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment