Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CG015_RS03165 Genome accession   NZ_CP022468
Coordinates   652153..653259 (+) Length   368 a.a.
NCBI ID   WP_129532222.1    Uniprot ID   -
Organism   Vibrio anguillarum strain MHK3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 647153..658259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG015_RS03130 (CG015_03115) - 647502..648101 (-) 600 WP_017042394.1 XTP/dITP diphosphatase -
  CG015_RS03135 (CG015_03120) - 648114..648545 (-) 432 WP_010320641.1 DUF4426 domain-containing protein -
  CG015_RS03140 (CG015_03125) yggU 648588..648878 (-) 291 WP_129532221.1 DUF167 family protein YggU -
  CG015_RS03145 (CG015_03130) - 648878..649435 (-) 558 WP_010320643.1 YggT family protein -
  CG015_RS03150 (CG015_03135) proC 649498..650316 (-) 819 WP_064624328.1 pyrroline-5-carboxylate reductase -
  CG015_RS03155 (CG015_03140) - 650378..651079 (-) 702 WP_017048292.1 YggS family pyridoxal phosphate-dependent enzyme -
  CG015_RS03160 (CG015_03145) pilT 651105..652142 (+) 1038 WP_013857819.1 type IV pilus twitching motility protein PilT Machinery gene
  CG015_RS03165 (CG015_03150) pilU 652153..653259 (+) 1107 WP_129532222.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CG015_RS03170 (CG015_03155) ruvX 653343..653771 (-) 429 WP_017045508.1 Holliday junction resolvase RuvX -
  CG015_RS03175 (CG015_03160) - 653854..654417 (-) 564 WP_129532223.1 YqgE/AlgH family protein -
  CG015_RS03180 (CG015_03165) gshB 654464..655417 (-) 954 WP_129532224.1 glutathione synthase -
  CG015_RS03185 (CG015_03170) rsmE 655431..656162 (-) 732 WP_013857814.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CG015_RS03190 (CG015_03175) endA 656271..656978 (-) 708 WP_013857813.1 deoxyribonuclease I -
  CG015_RS03195 (CG015_03180) - 657110..657610 (-) 501 WP_019282289.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41399.46 Da        Isoelectric Point: 6.5071

>NTDB_id=239707 CG015_RS03165 WP_129532222.1 652153..653259(+) (pilU) [Vibrio anguillarum strain MHK3]
MELNQCLEGMLTQKASDLYLTVGAPVLFRVDGELRAHGEKLSQTQVMALLDAMMDEDRRREFQRTREANFAVVRDIGRFR
VSAFFQRELPGAVIRRIETHIPTFEELKLPGVLQDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNSHRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVVGQQLIRDKNGKGRHGVFEILLNSPRISDLIRRGDLHELKATMGKSKEVGMQTFDQALY
QLVVEDKISEQDALHSADSANDLRLMLKTKRGEGISSGTLDNVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=239707 CG015_RS03165 WP_129532222.1 652153..653259(+) (pilU) [Vibrio anguillarum strain MHK3]
ATGGAACTGAACCAATGTCTTGAGGGTATGTTGACTCAAAAAGCTTCCGACTTGTACCTCACCGTAGGAGCGCCAGTGTT
ATTTCGCGTCGATGGAGAGTTAAGAGCTCATGGCGAAAAGTTATCCCAAACGCAGGTGATGGCGTTGTTAGATGCCATGA
TGGATGAGGATCGCCGCCGTGAATTTCAACGCACACGTGAAGCCAATTTTGCAGTAGTGAGAGATATTGGCCGTTTTCGT
GTCAGTGCTTTTTTTCAGCGTGAGCTGCCCGGTGCGGTGATCCGCCGTATTGAAACTCATATCCCCACTTTTGAAGAATT
AAAGTTACCGGGAGTGTTGCAAGATCTCTCGATTGCAAAGCGTGGGCTGGTGCTGGTGGTCGGCGCGACGGGGTCGGGCA
AATCGACCACCATGGCGGCCATGACGGGTTATCGTAACTCGCACCGAACAGGGCATATTTTAACAGTGGAAGATCCGATT
GAGTTTGTGCATGAGCATCAGCGTTGCATTGTGACCCAGCGAGAAGTGGGGTTGGATACCGAGAGTTACGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCTGATATGATTTTAATTGGTGAGATCCGTTCACGTGAAACGATGGAATATGCGATGA
CCTTTGCCGAGACAGGTCATTTATGTATGGCAACGTTACATGCTAACAATGCCAACCAAGCGCTTGAGCGAATTTTGCAT
TTGGTTCCTAAAGAGCAAAAAGAGCAGTTTTTGTTTGATCTGTCGATGAACCTCAAAGGCGTTGTGGGGCAGCAGTTGAT
CCGCGATAAAAATGGCAAGGGACGACATGGTGTGTTTGAGATTTTACTCAACAGCCCTCGAATTTCCGATTTGATCCGCC
GCGGTGATCTGCACGAATTGAAAGCGACGATGGGCAAATCAAAAGAAGTGGGGATGCAGACTTTTGACCAAGCACTATAT
CAACTGGTCGTTGAGGACAAAATCAGTGAACAAGATGCACTGCACAGCGCTGACTCGGCTAACGATTTACGTTTAATGTT
AAAAACAAAGCGTGGCGAAGGGATCAGCAGTGGCACATTGGATAACGTGCAGATTGATATGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

87.5

100

0.875
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

52.991

95.38

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.429

95.109

0.413
  pilT Pseudomonas aeruginosa PAK

42.478

92.12

0.391
  pilT Acinetobacter baylyi ADP1

41.935

92.663

0.389
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37

Multiple sequence alignment