Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C6668_RS12160 Genome accession   NZ_CP028166
Coordinates   2308328..2309308 (-) Length   326 a.a.
NCBI ID   WP_001295381.1    Uniprot ID   -
Organism   Escherichia coli strain CFSAN064036     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2303328..2314308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6668_RS12130 (C6668_12130) yggI 2303983..2304480 (+) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -
  C6668_RS12135 (C6668_12135) endA 2304575..2305282 (+) 708 WP_107148002.1 deoxyribonuclease I -
  C6668_RS12140 (C6668_12140) rsmE 2305362..2306093 (+) 732 WP_032187617.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C6668_RS12145 (C6668_12145) gshB 2306106..2307056 (+) 951 WP_107148003.1 glutathione synthase -
  C6668_RS12150 (C6668_12150) yqgE 2307165..2307728 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  C6668_RS12155 (C6668_12155) ruvX 2307728..2308144 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  C6668_RS26920 - 2308151..2308279 (-) 129 WP_001278270.1 hypothetical protein -
  C6668_RS12160 (C6668_12160) pilT 2308328..2309308 (-) 981 WP_001295381.1 type IV pilus twitching motility protein PilT Machinery gene
  C6668_RS12165 (C6668_12165) yggS 2309326..2310030 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  C6668_RS12170 (C6668_12170) yggT 2310048..2310614 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  C6668_RS12175 (C6668_12175) yggU 2310611..2310901 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  C6668_RS12180 (C6668_12180) rdgB 2310909..2311502 (+) 594 WP_107148004.1 XTP/dITP diphosphatase -
  C6668_RS12185 (C6668_12185) hemW 2311495..2312631 (+) 1137 WP_000239928.1 radical SAM family heme chaperone HemW -
  C6668_RS12190 (C6668_12190) yggM 2312786..2313793 (-) 1008 WP_107148005.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35926.14 Da        Isoelectric Point: 5.7654

>NTDB_id=238976 C6668_RS12160 WP_001295381.1 2308328..2309308(-) (pilT) [Escherichia coli strain CFSAN064036]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGMILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=238976 C6668_RS12160 WP_001295381.1 2308328..2309308(-) (pilT) [Escherichia coli strain CFSAN064036]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCATGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.912

97.546

0.448
  pilT Legionella pneumophila strain Lp02

45.912

97.546

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.062

98.773

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

37.048

100

0.377
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362

Multiple sequence alignment