Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   QMA0248_RS00925 Genome accession   NZ_CP022392
Coordinates   145644..146039 (+) Length   131 a.a.
NCBI ID   WP_003098836.1    Uniprot ID   A0A3L8GMQ5
Organism   Streptococcus iniae strain QMA0248     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 87560..156942 145644..146039 within 0


Gene organization within MGE regions


Location: 87560..156942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMA0248_RS00605 (QMA0248_0126) - 87909..88103 (-) 195 WP_000562208.1 DUF3173 family protein -
  QMA0248_RS00610 (QMA0248_0127) - 88172..89518 (-) 1347 WP_003098677.1 Rep family protein -
  QMA0248_RS00615 (QMA0248_0128) - 89868..90227 (-) 360 WP_003098679.1 hypothetical protein -
  QMA0248_RS00625 (QMA0248_0129) - 90837..91244 (+) 408 WP_003098684.1 helix-turn-helix domain-containing protein -
  QMA0248_RS00630 (QMA0248_0130) - 91338..91532 (+) 195 WP_016355751.1 hypothetical protein -
  QMA0248_RS00635 - 91529..91837 (-) 309 Protein_89 RNA polymerase sigma factor -
  QMA0248_RS00645 (QMA0248_0132) - 92603..93556 (-) 954 WP_003098714.1 IS30 family transposase -
  QMA0248_RS10510 - 93674..93946 (+) 273 Protein_91 collagen binding domain-containing protein -
  QMA0248_RS10515 (QMA0248_0133) - 94076..95380 (+) 1305 WP_411198206.1 Cna B-type domain-containing protein -
  QMA0248_RS00660 (QMA0248_0134) - 95549..96724 (+) 1176 WP_003099060.1 IS256 family transposase -
  QMA0248_RS10520 - 96985..97185 (+) 201 WP_080768722.1 ATP-binding cassette domain-containing protein -
  QMA0248_RS00675 (QMA0248_0135) - 97721..98488 (+) 768 WP_037583616.1 ABC transporter permease subunit -
  QMA0248_RS00680 (QMA0248_0136) - 98807..99829 (+) 1023 WP_003098721.1 IS30 family transposase -
  QMA0248_RS10390 - 100085..100171 (+) 87 Protein_97 ABC transporter ATP-binding protein -
  QMA0248_RS00690 (QMA0248_0137) - 100168..100398 (+) 231 Protein_98 hypothetical protein -
  QMA0248_RS00700 (QMA0248_0140) - 101624..101887 (+) 264 Protein_100 hypothetical protein -
  QMA0248_RS00705 (QMA0248_0141) - 102102..102551 (+) 450 WP_016355755.1 pyridoxamine 5'-phosphate oxidase family protein -
  QMA0248_RS00710 (QMA0248_0142) - 102653..103066 (+) 414 WP_003098726.1 DUF1722 domain-containing protein -
  QMA0248_RS00715 (QMA0248_0143) adhP 103126..104145 (-) 1020 WP_003098727.1 alcohol dehydrogenase AdhP -
  QMA0248_RS00720 - 104945..105298 (+) 354 WP_003098729.1 alpha/beta fold hydrolase -
  QMA0248_RS00725 - 105335..105733 (+) 399 WP_016355758.1 hypothetical protein -
  QMA0248_RS00730 (QMA0248_0147) - 105846..106376 (+) 531 WP_003098733.1 GNAT family N-acetyltransferase -
  QMA0248_RS00735 - 106596..107218 (+) 623 Protein_107 MFS transporter -
  QMA0248_RS00740 (QMA0248_0149) - 107490..108714 (+) 1225 Protein_108 peptidoglycan bridge formation glycyltransferase FemA/FemB family protein -
  QMA0248_RS00745 (QMA0248_0151) - 108795..109325 (+) 531 WP_003098764.1 NUDIX hydrolase -
  QMA0248_RS00750 (QMA0248_0152) - 109336..109959 (+) 624 WP_003098766.1 tetratricopeptide repeat protein -
  QMA0248_RS00755 (QMA0248_0153) - 110369..111268 (+) 900 WP_016355761.1 prenyltransferase -
  QMA0248_RS00760 (QMA0248_0154) - 111281..112492 (+) 1212 WP_003098771.1 NAD(P)/FAD-dependent oxidoreductase -
  QMA0248_RS00765 (QMA0248_0155) - 112579..114006 (+) 1428 WP_089180046.1 cytochrome ubiquinol oxidase subunit I -
  QMA0248_RS00770 (QMA0248_0156) cydB 114008..115027 (+) 1020 WP_003098776.1 cytochrome d ubiquinol oxidase subunit II -
  QMA0248_RS00775 (QMA0248_0157) cydD 115027..116745 (+) 1719 WP_016355762.1 thiol reductant ABC exporter subunit CydD -
  QMA0248_RS00780 (QMA0248_0158) cydC 116738..118483 (+) 1746 WP_089180047.1 thiol reductant ABC exporter subunit CydC -
  QMA0248_RS00785 (QMA0248_0159) - 118617..119594 (-) 978 WP_003098781.1 polyprenyl synthetase family protein -
  QMA0248_RS00790 (QMA0248_0160) ispE 119795..120646 (+) 852 WP_003098783.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
  QMA0248_RS00795 (QMA0248_0161) - 120714..121157 (+) 444 WP_003098785.1 zinc-dependent MarR family transcriptional regulator -
  QMA0248_RS00800 (QMA0248_0162) - 121162..121860 (+) 699 WP_003098787.1 metal ABC transporter ATP-binding protein -
  QMA0248_RS00805 (QMA0248_0163) - 121863..122678 (+) 816 WP_003098790.1 metal ABC transporter permease -
  QMA0248_RS00810 (QMA0248_0164) tyrS 122723..123979 (-) 1257 WP_003098791.1 tyrosine--tRNA ligase -
  QMA0248_RS00815 (QMA0248_0165) pbp1b 124073..126379 (+) 2307 WP_031239175.1 penicillin-binding protein PBP1B -
  QMA0248_RS00820 (QMA0248_0166) rpoB 126641..130204 (+) 3564 WP_089180048.1 DNA-directed RNA polymerase subunit beta -
  QMA0248_RS00825 (QMA0248_0167) rpoC 130302..133934 (+) 3633 WP_089180049.1 DNA-directed RNA polymerase subunit beta' -
  QMA0248_RS00830 (QMA0248_0168) - 134053..134418 (+) 366 WP_003098799.1 DUF1033 family protein -
  QMA0248_RS00835 (QMA0248_0169) comYA 134479..135420 (+) 942 WP_003098801.1 competence type IV pilus ATPase ComGA Machinery gene
  QMA0248_RS00840 (QMA0248_0170) comYB 135371..136387 (+) 1017 WP_259458648.1 competence type IV pilus assembly protein ComGB Machinery gene
  QMA0248_RS00845 (QMA0248_0171) comGC/cglC 136388..136714 (+) 327 WP_003098806.1 competence type IV pilus major pilin ComGC Machinery gene
  QMA0248_RS10465 (QMA0248_0172) comGD 136674..137102 (+) 429 WP_003098807.1 competence type IV pilus minor pilin ComGD -
  QMA0248_RS00855 (QMA0248_2083) comGE 137074..137367 (+) 294 WP_031239176.1 competence type IV pilus minor pilin ComGE -
  QMA0248_RS00860 (QMA0248_0173) comYF 137327..137776 (+) 450 WP_119773875.1 competence type IV pilus minor pilin ComGF Machinery gene
  QMA0248_RS00865 (QMA0248_0174) comGG 137766..138119 (+) 354 WP_003098812.1 competence type IV pilus minor pilin ComGG -
  QMA0248_RS00870 (QMA0248_0175) comYH 138181..139137 (+) 957 WP_003098815.1 class I SAM-dependent methyltransferase Machinery gene
  QMA0248_RS00875 (QMA0248_0176) - 139194..140390 (+) 1197 WP_003098816.1 acetate kinase -
  QMA0248_RS00880 (QMA0248_0177) - 140513..140716 (+) 204 WP_003098819.1 helix-turn-helix transcriptional regulator -
  QMA0248_RS00885 (QMA0248_0178) - 140729..141181 (+) 453 WP_003098820.1 hypothetical protein -
  QMA0248_RS00890 (QMA0248_0179) - 141191..141847 (+) 657 WP_003098822.1 CPBP family intramembrane glutamic endopeptidase -
  QMA0248_RS00895 (QMA0248_0180) proC 141881..142651 (-) 771 WP_003098824.1 pyrroline-5-carboxylate reductase -
  QMA0248_RS00900 (QMA0248_0181) pepA 142675..143742 (-) 1068 WP_003098826.1 glutamyl aminopeptidase -
  QMA0248_RS00905 (QMA0248_2084) - 143883..144167 (+) 285 WP_003098828.1 DUF4651 domain-containing protein -
  QMA0248_RS00910 (QMA0248_0182) - 144164..144481 (+) 318 WP_003098830.1 thioredoxin family protein -
  QMA0248_RS00915 (QMA0248_0183) ytpR 144498..145124 (+) 627 WP_003098832.1 YtpR family tRNA-binding protein -
  QMA0248_RS00920 (QMA0248_0184) - 145162..145572 (-) 411 WP_003098834.1 GNAT family N-acetyltransferase -
  QMA0248_RS00925 (QMA0248_0185) ssbA 145644..146039 (+) 396 WP_003098836.1 single-stranded DNA-binding protein Machinery gene
  QMA0248_RS00930 (QMA0248_0186) - 146513..147475 (+) 963 WP_003098839.1 ABC transporter substrate-binding protein -
  QMA0248_RS00935 (QMA0248_0187) - 147489..148463 (+) 975 WP_089180051.1 ABC transporter substrate-binding protein -
  QMA0248_RS00940 (QMA0248_0188) - 148473..149357 (+) 885 WP_003098842.1 ABC transporter permease -
  QMA0248_RS00945 (QMA0248_0189) - 149366..150169 (+) 804 WP_003098843.1 ABC transporter ATP-binding protein -
  QMA0248_RS00950 (QMA0248_0190) - 150230..150874 (-) 645 WP_003098844.1 deoxynucleoside kinase -
  QMA0248_RS00955 (QMA0248_0191) dusB 150892..151869 (-) 978 WP_003098845.1 tRNA dihydrouridine synthase DusB -
  QMA0248_RS00960 (QMA0248_0192) hslO 151856..152728 (-) 873 WP_003098846.1 Hsp33 family molecular chaperone HslO -
  QMA0248_RS00965 (QMA0248_0193) - 153079..156663 (+) 3585 WP_003098847.1 accessory Sec-dependent serine-rich glycoprotein adhesin -

Sequence


Protein


Download         Length: 131 a.a.        Molecular weight: 15061.41 Da        Isoelectric Point: 5.9574

>NTDB_id=238976 QMA0248_RS00925 WP_003098836.1 145644..146039(+) (ssbA) [Streptococcus iniae strain QMA0248]
MYNKVVMIGRLVAEPELIKTAKDKSICRVSLAVNRRYKNADGEKQVDFVPMILWGKLAETLVSYGSKGSLMSIDGELRTR
RYEKEGKSHFMMEILCQSFQLLESRAQRAMRENNADNELADLILDEEELPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=238976 QMA0248_RS00925 WP_003098836.1 145644..146039(+) (ssbA) [Streptococcus iniae strain QMA0248]
ATGTATAATAAAGTCGTAATGATTGGCCGCTTGGTTGCAGAACCAGAATTAATAAAAACAGCAAAGGATAAGTCTATTTG
TCGTGTTTCACTAGCAGTCAATAGGCGTTACAAAAATGCTGATGGTGAAAAACAGGTGGATTTTGTCCCAATGATACTTT
GGGGGAAATTAGCTGAGACACTCGTTTCTTATGGCAGCAAGGGAAGTTTGATGTCGATTGATGGTGAATTGCGTACAAGA
CGCTATGAAAAAGAGGGCAAATCTCATTTTATGATGGAGATTTTGTGTCAATCTTTTCAGTTATTAGAAAGTCGGGCACA
AAGAGCCATGCGGGAAAACAATGCTGACAATGAGTTAGCAGACCTCATTTTAGATGAGGAAGAATTACCTTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A3L8GMQ5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Streptococcus mutans UA159

73.282

100

0.733

  ssbB/cilA Streptococcus pneumoniae Rx1

67.176

100

0.672

  ssbB/cilA Streptococcus pneumoniae D39

67.176

100

0.672

  ssbB/cilA Streptococcus pneumoniae R6

67.176

100

0.672

  ssbB/cilA Streptococcus mitis NCTC 12261

67.176

100

0.672

  ssbB/cilA Streptococcus pneumoniae TIGR4

66.412

100

0.664

  ssbB/cilA Streptococcus mitis SK321

66.412

100

0.664

  ssbB Streptococcus sobrinus strain NIDR 6715-7

65.649

100

0.656

  ssbB Lactococcus lactis subsp. cremoris KW2

57.143

85.496

0.489


Multiple sequence alignment