Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CF168_RS14665 Genome accession   NZ_CP022358
Coordinates   3366422..3367459 (-) Length   345 a.a.
NCBI ID   WP_011716282.1    Uniprot ID   A0A2M7HST3
Organism   Shewanella bicestrii strain JAB-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3361422..3372459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CF168_RS14630 (CF168_14630) - 3361596..3361910 (+) 315 WP_011716287.1 hypothetical protein -
  CF168_RS14635 (CF168_14635) yciH 3362060..3362389 (+) 330 WP_011716286.1 stress response translation initiation inhibitor YciH -
  CF168_RS14640 (CF168_14640) - 3362458..3363021 (+) 564 WP_011621987.1 YqgE/AlgH family protein -
  CF168_RS14645 (CF168_14645) ruvX 3363050..3363475 (+) 426 WP_011621986.1 Holliday junction resolvase RuvX -
  CF168_RS14650 (CF168_14650) hemH 3363537..3364520 (-) 984 WP_011716285.1 ferrochelatase -
  CF168_RS14655 (CF168_14655) - 3364590..3365123 (-) 534 WP_041412578.1 glutathione peroxidase -
  CF168_RS14660 (CF168_14660) pilU 3365300..3366412 (-) 1113 WP_011716283.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CF168_RS14665 (CF168_14665) pilT 3366422..3367459 (-) 1038 WP_011716282.1 type IV pilus twitching motility protein PilT Machinery gene
  CF168_RS14670 (CF168_14670) - 3367495..3368211 (+) 717 WP_011716281.1 YggS family pyridoxal phosphate-dependent enzyme -
  CF168_RS22660 - 3368208..3368336 (-) 129 WP_276207210.1 hypothetical protein -
  CF168_RS14675 (CF168_14675) proC 3368433..3369251 (+) 819 WP_011716280.1 pyrroline-5-carboxylate reductase -
  CF168_RS14680 (CF168_14680) - 3369327..3369875 (+) 549 WP_011716279.1 YggT family protein -
  CF168_RS14685 (CF168_14685) yggU 3369875..3370165 (+) 291 WP_011716278.1 DUF167 family protein YggU -
  CF168_RS14690 (CF168_14690) - 3370234..3370668 (+) 435 WP_011716277.1 DUF4426 domain-containing protein -
  CF168_RS14695 (CF168_14695) rdgB 3370838..3371455 (+) 618 WP_011625625.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38356.02 Da        Isoelectric Point: 6.9097

>NTDB_id=238740 CF168_RS14665 WP_011716282.1 3366422..3367459(-) (pilT) [Shewanella bicestrii strain JAB-1]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMSKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=238740 CF168_RS14665 WP_011716282.1 3366422..3367459(-) (pilT) [Shewanella bicestrii strain JAB-1]
ATGGAAATCACTGAGTTATTAGCCTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATTAACCTGCCCGCGCTCGATCATCAGGGTGTACACAGCCTTGTGTACGACA
TTATGAATGATAAACAGCGTAAGGACTTTGAAGAGCATTTAGAAATCGACTTTTCGTTCGAAGTCCCTAATTTAGCGCGT
TTCCGTGTGAACGCCTTTAACCAATCCCGCGGCGCGGCGGCGGTATTTCGTACCATTCCCAGCGAAATTTTGTCACTCGA
GCAGTTAGGGGCGCCAGAGATTTTTAAAAAAATTTCCAGCTTTCCCCGCGGCTTAGTGCTTGTTACTGGGCCTACTGGTT
CGGGTAAGAGTACCACACTTGCGGCCATGGTGGATTACATCAATGAGAACCGCCACGACCATATCTTAACCATTGAAGAT
CCTATTGAATTCGTTCACCAGAATAAGCAATGTTTGATTAACCAACGGGAAGTGCATCGCCATACCCACAGCTTTAACGC
GGCGCTACGTAGCGCACTGCGTGAAGACCCTGACGTTATTCTCGTCGGTGAGATGCGTGACCTTGAAACCATTCGTCTGG
CTATGACGGCGGCTGAAACGGGTCACTTAGTTTTTGGTACCTTGCATACCACCTCGGCGGCTAAGACCATCGACCGTGTG
GTTGACGTTTTCCCTGCCGGTGAAAAGGACATGGTGCGTACCATGTTATCTGAATCATTACAGGCGGTTATTTCGCAAAC
CCTGATTAAGAAAATCGGTGGTGGCCGTGTGGCTGCCCACGAAATCATGATGGGTACGCCCGCTATCCGTAACCTTATCC
GTGAAGATAAAGTAGCGCAGATGTACTCGGCCATTCAAACGGGGATGGCCCATGGCATGCAAACGCTCGAACAGTGTCTG
CAAAACTTAGTGAACCGTGGCCTCATCACCCGTGAGGATGCCATGTCGAAGAGTTCAAACAAACAAGCGACGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2M7HST3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.592

99.42

0.791
  pilT Acinetobacter baumannii strain A118

79.592

99.42

0.791
  pilT Acinetobacter nosocomialis M2

79.592

99.42

0.791
  pilT Pseudomonas aeruginosa PAK

79.586

97.971

0.78
  pilT Acinetobacter baylyi ADP1

80.547

95.362

0.768
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

98.261

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.691

99.42

0.414
  pilU Vibrio cholerae strain A1552

41.369

97.391

0.403
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388

Multiple sequence alignment