Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CF168_RS14660 Genome accession   NZ_CP022358
Coordinates   3365300..3366412 (-) Length   370 a.a.
NCBI ID   WP_011716283.1    Uniprot ID   A0A220UQ50
Organism   Shewanella bicestrii strain JAB-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3360300..3371412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CF168_RS14625 (CF168_14625) - 3360856..3361416 (-) 561 WP_011621990.1 DUF4136 domain-containing protein -
  CF168_RS14630 (CF168_14630) - 3361596..3361910 (+) 315 WP_011716287.1 hypothetical protein -
  CF168_RS14635 (CF168_14635) yciH 3362060..3362389 (+) 330 WP_011716286.1 stress response translation initiation inhibitor YciH -
  CF168_RS14640 (CF168_14640) - 3362458..3363021 (+) 564 WP_011621987.1 YqgE/AlgH family protein -
  CF168_RS14645 (CF168_14645) ruvX 3363050..3363475 (+) 426 WP_011621986.1 Holliday junction resolvase RuvX -
  CF168_RS14650 (CF168_14650) hemH 3363537..3364520 (-) 984 WP_011716285.1 ferrochelatase -
  CF168_RS14655 (CF168_14655) - 3364590..3365123 (-) 534 WP_041412578.1 glutathione peroxidase -
  CF168_RS14660 (CF168_14660) pilU 3365300..3366412 (-) 1113 WP_011716283.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CF168_RS14665 (CF168_14665) pilT 3366422..3367459 (-) 1038 WP_011716282.1 type IV pilus twitching motility protein PilT Machinery gene
  CF168_RS14670 (CF168_14670) - 3367495..3368211 (+) 717 WP_011716281.1 YggS family pyridoxal phosphate-dependent enzyme -
  CF168_RS22660 - 3368208..3368336 (-) 129 WP_276207210.1 hypothetical protein -
  CF168_RS14675 (CF168_14675) proC 3368433..3369251 (+) 819 WP_011716280.1 pyrroline-5-carboxylate reductase -
  CF168_RS14680 (CF168_14680) - 3369327..3369875 (+) 549 WP_011716279.1 YggT family protein -
  CF168_RS14685 (CF168_14685) yggU 3369875..3370165 (+) 291 WP_011716278.1 DUF167 family protein YggU -
  CF168_RS14690 (CF168_14690) - 3370234..3370668 (+) 435 WP_011716277.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41173.39 Da        Isoelectric Point: 6.3725

>NTDB_id=238739 CF168_RS14660 WP_011716283.1 3365300..3366412(-) (pilU) [Shewanella bicestrii strain JAB-1]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESVMTEAQKKEFHSTRECNFAFAVKDLGR
FRVSAFWQRESPGCVMRRIETKIPEVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLQLYIDGEISYADALHHADSPNDLRLMIKLQNKEPTSSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=238739 CF168_RS14660 WP_011716283.1 3365300..3366412(-) (pilU) [Shewanella bicestrii strain JAB-1]
ATGGATGTCCGTCCGTTTTTAAAAGTCATGGTGGAGCGTAAAGCCTCGGACTTGTTTATTACCGCAGGTTTTCCGCCAAG
CGCCAAAATCGATGGCGAGTTACGCCCATTAGCCGAGAGTGCCTTCACGCCCGCGCAGTCTTTGGATTTTGTCGAATCTG
TAATGACTGAGGCGCAAAAGAAGGAGTTTCACAGCACTCGCGAGTGTAACTTTGCTTTTGCGGTTAAGGATTTAGGGCGT
TTCCGCGTCAGCGCATTCTGGCAGCGTGAATCTCCGGGATGCGTTATGCGCCGGATTGAGACCAAGATCCCTGAGGTAGA
GGACTTAAAACTGCCACCGATTTTAAAAGATCTGGTGATGAGTAAACGGGGTCTTATCATCATGGTCGGGGGGACGGGAA
CCGGTAAGTCGACCTCCTTGGCGGCGTTAGTGGGTTATCGTAACGCCCATGCCCGTGGTCATATCCTCACCATCGAAGAC
CCAGTGGAATTTGTGCACGATCATCGCAAAAGTATCATCACTCAACGCGAAGTGGGTATAGATACAGAATCCTTTGATGC
GGCGTTGAAGAGTTCGCTGCGTCAGGCGCCCGATGTGATTTTGATTGGTGAGATCCGTACTCAAGAAACCATGGAGTTTG
CGCTCTCCTTCGCCGAAACGGGTCACCTCTGTATGGCGACATTGCACGCAAACAACGCTAACCAAGCGTTAGACCGGATC
ATGCACTTAGTGCCTGAGAGTAAACACCAGCAGTTGCTATTCGACTTGTCACTCAACCTGCGCGGCATTGTGGCGCAGCA
ACTGGTGCCTAAGGTTGATGGTACAGGACGACGGGCAGCGATTGAAGTCTTGATCAATACGCCACGTGTCGCGAGCTTGA
TTGCTAAAAACGAGCTGCATTTGCTTAAAGAAACTATGGCTAAATCGAATGAGCAGGGGATGCAGACCTTCGACCAAGCC
TTGCTGCAACTCTATATCGATGGGGAAATCAGCTATGCCGATGCGCTTCACCATGCTGACTCGCCAAACGACTTACGTTT
AATGATCAAATTGCAAAATAAAGAGCCTACCAGTTCAAGCTTTATGGAAGGCGTGACCTTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A220UQ50

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Vibrio cholerae strain A1552

59.189

100

0.592
  pilU Acinetobacter baylyi ADP1

60.511

95.135

0.576
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.333

89.189

0.386
  pilT Vibrio cholerae strain A1552

43.333

89.189

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Acinetobacter nosocomialis M2

39.766

92.432

0.368
  pilT Acinetobacter baumannii D1279779

39.766

92.432

0.368
  pilT Acinetobacter baumannii strain A118

39.766

92.432

0.368
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365
  pilT Legionella pneumophila strain Lp02

41.358

87.568

0.362
  pilT Legionella pneumophila strain ERS1305867

41.358

87.568

0.362

Multiple sequence alignment