Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CFF01_RS13695 Genome accession   NZ_CP022272
Coordinates   3086708..3087745 (-) Length   345 a.a.
NCBI ID   WP_033539855.1    Uniprot ID   A0AAC9TZ45
Organism   Shewanella marisflavi strain EP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3081708..3092745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFF01_RS13670 (CFF01_13670) ruvX 3081926..3082345 (+) 420 WP_088905157.1 Holliday junction resolvase RuvX -
  CFF01_RS13680 (CFF01_13680) - 3082977..3083624 (-) 648 WP_088905159.1 DUF4145 domain-containing protein -
  CFF01_RS13685 (CFF01_13685) hemH 3084359..3085366 (-) 1008 WP_088905160.1 ferrochelatase -
  CFF01_RS13690 (CFF01_13690) pilU 3085583..3086695 (-) 1113 WP_033539856.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CFF01_RS13695 (CFF01_13695) pilT 3086708..3087745 (-) 1038 WP_033539855.1 type IV pilus twitching motility protein PilT Machinery gene
  CFF01_RS13700 (CFF01_13700) - 3087781..3088482 (+) 702 WP_088905161.1 YggS family pyridoxal phosphate-dependent enzyme -
  CFF01_RS13705 (CFF01_13705) proC 3088549..3089367 (+) 819 WP_088905162.1 pyrroline-5-carboxylate reductase -
  CFF01_RS13710 (CFF01_13710) - 3089425..3089973 (+) 549 WP_033539852.1 YggT family protein -
  CFF01_RS13715 (CFF01_13715) yggU 3089974..3090264 (+) 291 WP_088905163.1 DUF167 family protein YggU -
  CFF01_RS13720 (CFF01_13720) - 3090351..3090785 (+) 435 WP_088905164.1 DUF4426 domain-containing protein -
  CFF01_RS13725 (CFF01_13725) rdgB 3090924..3091523 (+) 600 WP_088905958.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  CFF01_RS13730 (CFF01_13730) hemW 3091523..3092662 (+) 1140 WP_088905165.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38273.83 Da        Isoelectric Point: 6.9095

>NTDB_id=237799 CFF01_RS13695 WP_033539855.1 3086708..3087745(-) (pilT) [Shewanella marisflavi strain EP1]
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEKLGAPDIFKKIASFPRGLVLVTGPTGSGKSTTLAGMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKSMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGLITREDAMSKSSNKNASF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=237799 CFF01_RS13695 WP_033539855.1 3086708..3087745(-) (pilT) [Shewanella marisflavi strain EP1]
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAACACAACGCGTCGGATCTACACCTTTCAGCGGGAGTATCTCCCAT
GATACGTGTTGATGGCGAGGTCAGAAAGATTAATCTGCCGGCCCTGGATCATCAAGGCGTACATAGCCTCGTTTATGACA
TCATGAACGACAAACAGCGTAAAGATTATGAAGAGCACTTAGAGATAGATTTCTCGTTCGAAGTGCCCAATTTAGCTCGC
TTCCGTGTGAACGCCTTTAACCAATCGCGCGGCGCCGCGGCGGTGTTTCGTACTATTCCCAGCGAGATCTTAAGCCTGGA
AAAACTCGGTGCCCCCGATATTTTCAAAAAGATTGCCAGCTTCCCTCGTGGCCTGGTGCTGGTAACCGGCCCCACAGGTT
CGGGTAAGAGTACCACCCTGGCGGGCATGATAGATTATGTGAACGAAAACCGTCACGACCATATCCTGACCATCGAAGAC
CCTATCGAATTCGTGCACCAGAATAAGCAATGTCTGATCAACCAGAGGGAAGTGCACAGACACACCCACAGCTTCAACGC
GGCGCTGCGTAGCGCACTGCGTGAAGACCCGGACGTGATCCTGGTGGGTGAGATGCGTGACCTTGAAACCATTCGTCTGG
CCATGACGGCGGCGGAAACCGGTCACTTAGTATTCGGTACACTGCACACCACCTCAGCGGCGAAAACCATTGACCGTGTG
GTCGACGTTTTCCCTGAAGGTGAAAAGAGTATGGTACGTACCATGTTGTCTGAATCACTGCAGGCGGTTATCTCGCAAAC
CCTGATCAAAAAGGTAGGCGGCGGCCGTGTGGCAGCTCACGAGATCATGATGGGTACACCGGCTATTCGTAACCTGATCC
GTGAAGACAAGGTGGCCCAGATGTACTCGGCCATTCAAACGGGTATGGCTCACGGGATGCAGACTCTGGATCAATGCTTG
CAGAATCTGGTCAATCGCGGCCTGATCACCCGTGAAGATGCAATGTCTAAGAGTTCTAATAAAAACGCTAGTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.138

96.812

0.786
  pilT Acinetobacter baumannii D1279779

81.138

96.812

0.786
  pilT Acinetobacter baumannii strain A118

81.138

96.812

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.107

97.971

0.765
  pilT Pseudomonas aeruginosa PAK

78.107

97.971

0.765
  pilT Acinetobacter baylyi ADP1

79.635

95.362

0.759
  pilT Legionella pneumophila strain Lp02

75.146

99.13

0.745
  pilT Legionella pneumophila strain ERS1305867

75.146

99.13

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.186

99.71

0.69
  pilT Neisseria gonorrhoeae MS11

68.895

99.71

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

41.691

99.42

0.414
  pilU Vibrio cholerae strain A1552

40.233

99.42

0.4
  pilU Acinetobacter baylyi ADP1

39.535

99.71

0.394

Multiple sequence alignment