Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CFF01_RS13690 Genome accession   NZ_CP022272
Coordinates   3085583..3086695 (-) Length   370 a.a.
NCBI ID   WP_033539856.1    Uniprot ID   A0AAC9U1X7
Organism   Shewanella marisflavi strain EP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3080583..3091695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFF01_RS13660 (CFF01_13660) yciH 3081001..3081330 (+) 330 WP_033538653.1 stress response translation initiation inhibitor YciH -
  CFF01_RS13665 (CFF01_13665) - 3081347..3081904 (+) 558 WP_088905156.1 YqgE/AlgH family protein -
  CFF01_RS13670 (CFF01_13670) ruvX 3081926..3082345 (+) 420 WP_088905157.1 Holliday junction resolvase RuvX -
  CFF01_RS13680 (CFF01_13680) - 3082977..3083624 (-) 648 WP_088905159.1 DUF4145 domain-containing protein -
  CFF01_RS13685 (CFF01_13685) hemH 3084359..3085366 (-) 1008 WP_088905160.1 ferrochelatase -
  CFF01_RS13690 (CFF01_13690) pilU 3085583..3086695 (-) 1113 WP_033539856.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CFF01_RS13695 (CFF01_13695) pilT 3086708..3087745 (-) 1038 WP_033539855.1 type IV pilus twitching motility protein PilT Machinery gene
  CFF01_RS13700 (CFF01_13700) - 3087781..3088482 (+) 702 WP_088905161.1 YggS family pyridoxal phosphate-dependent enzyme -
  CFF01_RS13705 (CFF01_13705) proC 3088549..3089367 (+) 819 WP_088905162.1 pyrroline-5-carboxylate reductase -
  CFF01_RS13710 (CFF01_13710) - 3089425..3089973 (+) 549 WP_033539852.1 YggT family protein -
  CFF01_RS13715 (CFF01_13715) yggU 3089974..3090264 (+) 291 WP_088905163.1 DUF167 family protein YggU -
  CFF01_RS13720 (CFF01_13720) - 3090351..3090785 (+) 435 WP_088905164.1 DUF4426 domain-containing protein -
  CFF01_RS13725 (CFF01_13725) rdgB 3090924..3091523 (+) 600 WP_088905958.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41226.27 Da        Isoelectric Point: 6.1913

>NTDB_id=237798 CFF01_RS13690 WP_033539856.1 3085583..3086695(-) (pilU) [Shewanella marisflavi strain EP1]
MEVRPFLKVMVERKASDLFITAGFPPSAKVDGEVRPLAENPFTPAQSLEFVEALMTEAQRKEFHETRECNFAYGEKDLGR
FRVSAFWQRESPGCVMRRIETKIPLVEDLHLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKSDGTGRRAAIEVLINTPRVSSIIQKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVEGEISYSDALHHADSPNDLRLMIKLQSSESASSGFMEGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=237798 CFF01_RS13690 WP_033539856.1 3085583..3086695(-) (pilU) [Shewanella marisflavi strain EP1]
ATGGAAGTTCGTCCATTTTTAAAGGTGATGGTAGAGCGTAAGGCATCGGATCTATTCATCACGGCTGGTTTTCCTCCCAG
CGCCAAGGTTGATGGTGAGGTGCGACCCCTTGCCGAAAATCCCTTTACGCCGGCTCAGTCACTGGAGTTTGTCGAAGCCT
TGATGACAGAGGCTCAGCGCAAAGAGTTTCACGAGACCCGCGAATGTAACTTCGCCTATGGCGAGAAGGATCTGGGGCGT
TTCCGTGTTAGCGCCTTCTGGCAGCGAGAGTCGCCAGGTTGCGTTATGCGTCGCATCGAGACCAAGATCCCGCTAGTCGA
AGATCTTCATCTGCCGCCAATTTTGAAAGACTTAGTGATGAGTAAGCGCGGTCTTATCATCATGGTGGGTGGTACGGGTA
CGGGTAAGTCGACTTCGCTCGCGGCCCTGGTGGGCTATCGCAATGCCCATGCTCGCGGCCATATTCTGACCATCGAAGAT
CCGGTGGAATTTGTCCATGACCATCGCAAGAGCATCATCACTCAGCGAGAGGTAGGTATAGATACTGATTCTTTCGATGC
TGCGCTAAAGAGTTCTTTGCGTCAGGCACCAGACGTGATTCTGATCGGTGAGATCCGAACCCAGGAAACCATGGAGTTTG
CCCTGTCGTTCGCCGAAACCGGTCACCTCTGTATGGCAACCTTGCACGCCAACAACGCTAACCAGGCGCTGGACCGCATC
ATGCACCTGGTGCCAGAGAGTAAGCATCAGCAGTTACTGTTCGACTTGTCGTTAAACCTGCGCGGCATTGTGGCGCAGCA
GTTGGTACCAAAGAGTGACGGTACGGGTCGCCGTGCGGCGATCGAGGTATTGATTAACACCCCAAGGGTTTCCAGCATTA
TCCAGAAGAACGAGCTGCATCTGCTTAAGGAAACCATGGCGAAATCCAACGAGCAGGGGATGCAGACCTTCGACCAGGCG
CTACTCAAGCTCTATGTGGAAGGTGAGATCAGCTACTCGGATGCATTGCATCACGCCGACTCGCCAAACGATCTGCGCTT
GATGATTAAGCTGCAAAGCTCAGAGTCTGCAAGTTCAGGCTTTATGGAAGGTGTGACGCTGGATCTCGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.186

100

0.614
  pilU Acinetobacter baylyi ADP1

60.446

97.027

0.586
  pilU Vibrio cholerae strain A1552

57.838

100

0.578
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.444

97.297

0.384
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365

Multiple sequence alignment