Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CEW83_RS13360 Genome accession   NZ_CP022187
Coordinates   2922023..2923066 (+) Length   347 a.a.
NCBI ID   WP_108949787.1    Uniprot ID   A0A2U8GRE6
Organism   Parazoarcus communis strain TSPY31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2917023..2928066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEW83_RS13330 (CEW83_13325) - 2917187..2918146 (+) 960 WP_108949781.1 aspartate carbamoyltransferase catalytic subunit -
  CEW83_RS13335 (CEW83_13330) - 2918157..2919434 (+) 1278 WP_108949782.1 dihydroorotase -
  CEW83_RS13340 (CEW83_13335) - 2919458..2919754 (-) 297 WP_108949783.1 DUF167 domain-containing protein -
  CEW83_RS13345 (CEW83_13340) - 2919751..2920332 (-) 582 WP_108949784.1 YggT family protein -
  CEW83_RS13350 (CEW83_13345) proC 2920339..2921163 (-) 825 WP_108949785.1 pyrroline-5-carboxylate reductase -
  CEW83_RS13355 (CEW83_13350) - 2921204..2921920 (-) 717 WP_108949786.1 YggS family pyridoxal phosphate-dependent enzyme -
  CEW83_RS13360 (CEW83_13355) pilT 2922023..2923066 (+) 1044 WP_108949787.1 type IV pilus twitching motility protein PilT Machinery gene
  CEW83_RS13365 (CEW83_13360) pilU 2923164..2924297 (+) 1134 WP_108949788.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CEW83_RS13370 (CEW83_13365) - 2924352..2925533 (-) 1182 WP_108949789.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  CEW83_RS13375 (CEW83_13370) - 2925788..2926003 (-) 216 WP_108949790.1 hypothetical protein -
  CEW83_RS13380 (CEW83_13375) - 2926000..2927034 (-) 1035 WP_108951400.1 formate dehydrogenase subunit gamma -
  CEW83_RS13385 (CEW83_13380) - 2927046..2927273 (-) 228 WP_108949791.1 hypothetical protein -
  CEW83_RS13390 (CEW83_13385) fdh3B 2927270..2927878 (-) 609 WP_108949792.1 formate dehydrogenase FDH3 subunit beta -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38570.22 Da        Isoelectric Point: 7.0157

>NTDB_id=237470 CEW83_RS13360 WP_108949787.1 2922023..2923066(+) (pilT) [Parazoarcus communis strain TSPY31]
MDITELLAFAVKNKASDLHLSSGLAPMIRVHGDVRRINLPPMEHKDVHAMVYDIMNDGQRKQYEETWECDFSFAIPNLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEELNCPKIFKDIANQPRGIVLVTGPTGSGKSTTLAAMVDYVNENEYGHILTVED
PIEFVHESKRCLINQREVHRDTMSFTNALRASLREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGAGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQNVGMQTLDQC
LSDLVRRNIVSNAEARLKAQNKESFAG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=237470 CEW83_RS13360 WP_108949787.1 2922023..2923066(+) (pilT) [Parazoarcus communis strain TSPY31]
ATGGACATCACAGAACTCCTCGCCTTTGCGGTCAAGAACAAGGCCTCCGACCTTCACCTCTCGTCAGGCCTGGCGCCGAT
GATCCGGGTGCATGGAGACGTCCGGCGCATCAACCTGCCGCCGATGGAACACAAGGACGTCCATGCCATGGTGTATGACA
TCATGAACGACGGCCAGCGCAAGCAGTACGAGGAAACCTGGGAGTGCGACTTTTCCTTCGCCATTCCCAATCTGGCACGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCGGGCGCCGTGTTCCGGACCATTCCGTCCAAGGTGCTCACGCTCGA
AGAGCTGAACTGCCCGAAGATCTTCAAGGACATTGCCAACCAGCCGCGCGGCATCGTGCTCGTCACCGGCCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCCATGGTGGACTACGTCAATGAAAACGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAGTTCGTGCACGAATCCAAGCGCTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGATGTCCTTCACCAA
TGCGCTGCGCGCCTCGCTGCGTGAAGACCCCGACGTCATCCTGGTGGGCGAGATGCGCGACCTCGAAACCATCCGTCTGG
CGCTCACCGCGGCCGAGACGGGCCATCTGGTGTTCGGCACGCTGCACACCTCGTCTGCCGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCGGCGGCCGAGAAGGACATGGTGCGTTCGATGCTCTCCGAGTCGCTGCGTGCGGTGATCTCGCAGAC
ACTGCTCAAGACCAAGGACGGCGCCGGCCGCGTGGCTGCGCACGAAATCATGATCGGCACCCCTGCAATCCGCAACCTGA
TCCGCGAGAACAAGATCGCACAGATGTACTCCGCGATTCAGACCGGACAGAACGTCGGCATGCAGACGCTCGACCAGTGC
CTGTCCGATCTGGTGCGGCGTAATATCGTGTCCAACGCCGAAGCCAGGCTGAAGGCACAGAACAAGGAAAGCTTCGCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8GRE6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.42

99.424

0.79
  pilT Acinetobacter baumannii strain A118

79.42

99.424

0.79
  pilT Acinetobacter nosocomialis M2

79.42

99.424

0.79
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Acinetobacter baylyi ADP1

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Legionella pneumophila strain Lp02

72.754

99.424

0.723
  pilT Legionella pneumophila strain ERS1305867

72.754

99.424

0.723
  pilT Neisseria meningitidis 8013

71.387

99.712

0.712
  pilT Neisseria gonorrhoeae MS11

71.098

99.712

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.235

97.983

0.669
  pilT Vibrio cholerae strain A1552

68.235

97.983

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.023

99.712

0.519
  pilU Acinetobacter baylyi ADP1

42.308

97.406

0.412
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.298

97.695

0.403

Multiple sequence alignment