Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CEW83_RS13365 Genome accession   NZ_CP022187
Coordinates   2923164..2924297 (+) Length   377 a.a.
NCBI ID   WP_108949788.1    Uniprot ID   A0A2U8GUF9
Organism   Parazoarcus communis strain TSPY31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2918164..2929297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEW83_RS13340 (CEW83_13335) - 2919458..2919754 (-) 297 WP_108949783.1 DUF167 domain-containing protein -
  CEW83_RS13345 (CEW83_13340) - 2919751..2920332 (-) 582 WP_108949784.1 YggT family protein -
  CEW83_RS13350 (CEW83_13345) proC 2920339..2921163 (-) 825 WP_108949785.1 pyrroline-5-carboxylate reductase -
  CEW83_RS13355 (CEW83_13350) - 2921204..2921920 (-) 717 WP_108949786.1 YggS family pyridoxal phosphate-dependent enzyme -
  CEW83_RS13360 (CEW83_13355) pilT 2922023..2923066 (+) 1044 WP_108949787.1 type IV pilus twitching motility protein PilT Machinery gene
  CEW83_RS13365 (CEW83_13360) pilU 2923164..2924297 (+) 1134 WP_108949788.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CEW83_RS13370 (CEW83_13365) - 2924352..2925533 (-) 1182 WP_108949789.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  CEW83_RS13375 (CEW83_13370) - 2925788..2926003 (-) 216 WP_108949790.1 hypothetical protein -
  CEW83_RS13380 (CEW83_13375) - 2926000..2927034 (-) 1035 WP_108951400.1 formate dehydrogenase subunit gamma -
  CEW83_RS13385 (CEW83_13380) - 2927046..2927273 (-) 228 WP_108949791.1 hypothetical protein -
  CEW83_RS13390 (CEW83_13385) fdh3B 2927270..2927878 (-) 609 WP_108949792.1 formate dehydrogenase FDH3 subunit beta -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42174.55 Da        Isoelectric Point: 6.3145

>NTDB_id=237471 CEW83_RS13365 WP_108949788.1 2923164..2924297(+) (pilU) [Parazoarcus communis strain TSPY31]
MERDQALKFMHDLLRLMLQKNGSDLFINSGFPPAIKIDGRIVPQSNQALSPAHTAELARAIMNDRQAAEFEATKECNFAI
SPAGIGRFRANAYIQQGRVGLVLRTIALKIPTFDELGLPAVLRDIAMAKRGLVIFVGGTGTGKTTSLAAMVDYRNENSYG
HIITVEDPIEYVHQHKNCIISQREVGIDTENWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEERRSQLLMDLSLNLRSMISQRLLPLKDRKGRIPAVEVLLNSPLISDLIFKGEVPGIKDIMKRSRELG
MQTFDQSLFDLYEEGMITYEDALRNADSVNDLRLQIKLNSKLGDKDMSSGIQHLDIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=237471 CEW83_RS13365 WP_108949788.1 2923164..2924297(+) (pilU) [Parazoarcus communis strain TSPY31]
ATGGAACGCGATCAGGCACTCAAGTTCATGCACGACCTGCTGCGGCTGATGCTGCAGAAGAACGGCTCCGACCTTTTCAT
CAACTCCGGATTCCCGCCGGCAATCAAGATCGACGGTCGCATCGTGCCGCAGTCGAATCAGGCGCTGAGCCCCGCCCACA
CCGCCGAACTCGCCCGCGCGATCATGAACGATCGCCAGGCTGCCGAGTTCGAGGCCACCAAGGAATGCAACTTCGCGATC
TCGCCGGCCGGCATCGGCCGCTTCCGCGCCAATGCCTATATCCAGCAGGGGCGCGTCGGCCTGGTCCTGCGGACCATCGC
GCTGAAGATTCCCACTTTCGACGAACTGGGCCTGCCGGCCGTGCTGCGTGACATCGCAATGGCCAAACGCGGCCTGGTGA
TTTTCGTCGGCGGGACGGGTACAGGCAAGACGACCTCGCTGGCGGCGATGGTCGATTACCGCAACGAGAACAGCTACGGG
CACATCATCACCGTAGAAGACCCGATCGAGTATGTGCATCAGCACAAGAACTGCATCATTTCCCAGCGTGAGGTGGGCAT
CGACACCGAGAACTGGGAGGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGACTACGCGATTGCCTTCGCCGAAACCGGCCACCTGTGTCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCCATCGACCGCATCATCAACTTCTTCCCGGAAGAACGTCGCTCGCAGTTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGATGATCTCGCAACGCCTGCTGCCGCTCAAGGACCGCAAGGGCCGCATCCCGGCAGTCGAAGTCCTGCTCAATT
CGCCACTGATCTCCGATCTCATCTTCAAGGGTGAAGTGCCGGGCATCAAGGACATCATGAAGCGTTCGCGCGAGCTCGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTCTACGAGGAAGGCATGATCACCTACGAGGATGCACTGCGAAACGCCGA
TTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCAAGCTGGGCGACAAGGACATGAGCTCGGGCATCCAGCACC
TCGACATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8GUF9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.538

96.552

0.594
  pilU Acinetobacter baylyi ADP1

58.12

93.103

0.541
  pilU Vibrio cholerae strain A1552

55.278

95.491

0.528
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.575

94.96

0.414
  pilT Acinetobacter baylyi ADP1

42.73

89.39

0.382
  pilT Acinetobacter nosocomialis M2

42.9

87.798

0.377
  pilT Acinetobacter baumannii strain A118

42.9

87.798

0.377
  pilT Acinetobacter baumannii D1279779

42.9

87.798

0.377
  pilT Pseudomonas stutzeri DSM 10701

42.09

88.859

0.374
  pilT Pseudomonas aeruginosa PAK

41.493

88.859

0.369
  pilT Legionella pneumophila strain ERS1305867

41.39

87.798

0.363
  pilT Legionella pneumophila strain Lp02

41.39

87.798

0.363

Multiple sequence alignment