Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   UN84_RS15425 Genome accession   NZ_CP022134
Coordinates   3258426..3259463 (-) Length   345 a.a.
NCBI ID   WP_046079379.1    Uniprot ID   -
Organism   Halomonas sp. HG01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3253426..3264463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UN84_RS15410 tyrS 3254651..3255850 (-) 1200 WP_046079382.1 tyrosine--tRNA ligase -
  UN84_RS15415 - 3255962..3257092 (+) 1131 WP_046079381.1 anhydro-N-acetylmuramic acid kinase -
  UN84_RS15420 pilU 3257257..3258399 (-) 1143 WP_046079380.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  UN84_RS15425 pilT 3258426..3259463 (-) 1038 WP_046079379.1 type IV pilus twitching motility protein PilT Machinery gene
  UN84_RS15430 - 3259518..3260225 (+) 708 WP_046079378.1 YggS family pyridoxal phosphate-dependent enzyme -
  UN84_RS15435 proC 3260287..3261111 (+) 825 WP_046079377.1 pyrroline-5-carboxylate reductase -
  UN84_RS15440 - 3261158..3261751 (+) 594 WP_035593026.1 YggT family protein -
  UN84_RS15445 - 3261934..3263091 (+) 1158 WP_046079376.1 homoserine O-acetyltransferase -
  UN84_RS15450 metW 3263091..3263684 (+) 594 WP_035593045.1 methionine biosynthesis protein MetW -
  UN84_RS15455 sfsA 3263720..3264463 (-) 744 WP_046079375.1 DNA/RNA nuclease SfsA -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38207.71 Da        Isoelectric Point: 6.4218

>NTDB_id=237156 UN84_RS15425 WP_046079379.1 3258426..3259463(-) (pilT) [Halomonas sp. HG01]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMDDREVRKLIYDILADRQRRDFEEYLETDFSFEVPGVSR
FRVNVFNQARGAGAVFRTIPSEVLTMEDLGMGETFRRLAMLPRGLVLVTGPTGSGKSTTLAAMIDHINDNRFEHILTIED
PVEFVHRSKRCLINQREVHRDTQGFAPALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGDEKAMVRSMLSESLQAVISQTLLKRQGGGRVAAHEILIATSAVRNLIREDKVAQIYSALQTGGNLGMQTLDTAL
SRLVADNVVAREEAQTKARATLAHQ

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=237156 UN84_RS15425 WP_046079379.1 3258426..3259463(-) (pilT) [Halomonas sp. HG01]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAACGCCTCGGACCTTCACCTTTCGGCCGGCCTGCCGCCGAT
GATCCGGGTCGACGGCGACATTCGGCGGCTCAACGTGCCGGCCATGGACGATCGTGAGGTCCGCAAGCTGATCTACGATA
TCCTCGCCGACCGTCAGCGGCGCGACTTCGAGGAGTACCTGGAAACCGACTTCTCCTTCGAGGTGCCGGGCGTCTCGCGC
TTTCGCGTCAACGTCTTCAACCAGGCCCGCGGCGCCGGGGCGGTGTTCCGCACCATTCCCTCCGAGGTGCTGACCATGGA
GGATCTGGGCATGGGCGAGACGTTCCGCCGCCTGGCCATGCTGCCGCGCGGCCTGGTGCTGGTGACCGGTCCTACCGGCT
CGGGCAAGAGCACCACCCTGGCGGCGATGATCGACCACATCAACGACAACCGCTTCGAGCATATCCTGACCATCGAGGAT
CCGGTGGAATTCGTGCATCGCAGCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCATCGCGACACCCAGGGCTTCGCCCC
GGCGCTGCGCAGTGCGCTGCGCGAGGACCCGGACGTGATCCTGGTCGGCGAGATGCGCGATCTGGAGACCATCCGGCTGG
CGCTGACCGCGGCCGAGACCGGCCATCTGGTGTTCGGCACCCTGCATACCACCTCGGCGGCCAAGACCATCGACCGGATC
ATCGATGTCTTCCCGGGGGACGAGAAGGCCATGGTGCGCTCGATGCTCTCCGAGTCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTCAAGCGCCAGGGCGGCGGCCGGGTGGCGGCCCACGAGATCCTGATCGCCACCTCGGCCGTGCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATCTACTCGGCGCTGCAGACCGGCGGTAACCTGGGCATGCAGACCCTGGATACGGCGCTG
TCGCGGCTGGTGGCGGACAACGTGGTCGCCCGTGAGGAAGCCCAGACCAAGGCGCGAGCCACGCTCGCGCATCAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.056

98.261

0.777
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Acinetobacter baumannii D1279779

76.106

98.261

0.748
  pilT Acinetobacter baumannii strain A118

76.106

98.261

0.748
  pilT Acinetobacter nosocomialis M2

75.811

98.261

0.745
  pilT Acinetobacter baylyi ADP1

75

98.551

0.739
  pilT Legionella pneumophila strain Lp02

69.231

97.971

0.678
  pilT Legionella pneumophila strain ERS1305867

69.231

97.971

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.681

0.667
  pilT Vibrio cholerae strain A1552

68.249

97.681

0.667
  pilT Neisseria meningitidis 8013

66.471

98.551

0.655
  pilT Neisseria gonorrhoeae MS11

66.176

98.551

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.802

98.261

0.519
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.101

0.403
  pilU Acinetobacter baylyi ADP1

40.882

98.551

0.403
  pilU Vibrio cholerae strain A1552

39.701

97.101

0.386

Multiple sequence alignment