Detailed information NTG0237155

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: UN84_RS15420
• Genome accession: NZ_CP022134
• Coordinates: 3257257..3258399 (-)
• Length: 380 a.a.
• NCBI ID: WP_046079380.1
• Uniprot ID: -
• Organism: Halomonas sp. HG01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3252257..3263399

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
UN84_RS15400	-	3252318..3253148 (+)	831	WP_046078425.1	Tim44-like domain-containing protein	-
UN84_RS15410	tyrS	3254651..3255850 (-)	1200	WP_046079382.1	tyrosine--tRNA ligase	-
UN84_RS15415	-	3255962..3257092 (+)	1131	WP_046079381.1	anhydro-N-acetylmuramic acid kinase	-
UN84_RS15420	pilU	3257257..3258399 (-)	1143	WP_046079380.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
UN84_RS15425	pilT	3258426..3259463 (-)	1038	WP_046079379.1	type IV pilus twitching motility protein PilT	Machinery gene
UN84_RS15430	-	3259518..3260225 (+)	708	WP_046079378.1	YggS family pyridoxal phosphate-dependent enzyme	-
UN84_RS15435	proC	3260287..3261111 (+)	825	WP_046079377.1	pyrroline-5-carboxylate reductase	-
UN84_RS15440	-	3261158..3261751 (+)	594	WP_035593026.1	YggT family protein	-
UN84_RS15445	-	3261934..3263091 (+)	1158	WP_046079376.1	homoserine O-acetyltransferase	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42007.11 Da
• Isoelectric Point: 5.4305

>NTDB_id=237155 UN84_RS15420 WP_046079380.1 3257257..3258399(-) (pilU) [Halomonas sp. HG01]
MTAKEWLYQLLDIMIEKEASDLLISVGAPPTLKMAGQLMPMGEEGLSVAQVGELVAAAMPENHRARFDDEQEANFALSLS
GRGRFRVSAFRQRNQPAMVLRRIAFEIPHLEELSLPEVLGELANIKRGLVFVVGGTGTGKSTTLASMIQQRNETAGGHII
SIEDPIEYVHPHRRAIINQREVGIDTESFEVALKNTLRQAPDVILIGEIRTRETMEHALAFAETGHLCLATLHANNANQA
LERIIHFFPHERHDQVWLDLSLNLQAIVAQQLLPTQAGGRCPAIEVLLKSPLIADLIRKGEVADVKNVMARSRDMGMQTF
DQALYDLYKKGAISEEVALVHADSANDLRMMIKYGDEQGVGLAQAKEAASHLSLRGEDDY

Nucleotide

• Length: 1143 bp

>NTDB_id=237155 UN84_RS15420 WP_046079380.1 3257257..3258399(-) (pilU) [Halomonas sp. HG01]
ATGACCGCGAAGGAATGGCTGTATCAGCTGCTCGACATCATGATCGAGAAGGAAGCCTCGGACCTGCTGATCTCGGTGGG
CGCGCCGCCGACGCTGAAGATGGCCGGTCAGCTGATGCCCATGGGCGAAGAAGGGCTGTCGGTGGCGCAGGTCGGCGAGC
TGGTGGCGGCGGCAATGCCTGAGAATCACCGGGCGCGCTTCGACGATGAGCAGGAGGCCAATTTCGCGCTCAGCCTGTCG
GGGCGGGGGCGCTTCCGCGTCAGTGCCTTCCGCCAGCGTAACCAGCCGGCCATGGTGCTCCGTCGCATCGCCTTCGAGAT
TCCCCATCTCGAGGAACTGTCGCTGCCGGAGGTGCTCGGCGAGCTGGCCAACATCAAGCGCGGGCTGGTGTTCGTGGTCG
GCGGCACCGGCACCGGCAAGTCGACGACCCTGGCGTCGATGATCCAGCAGCGCAACGAGACCGCCGGCGGCCACATCATC
AGCATCGAGGATCCGATCGAGTACGTGCACCCCCACCGCCGAGCGATCATCAACCAGCGCGAGGTCGGCATCGACACCGA
GTCGTTCGAGGTGGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCCTGATCGGCGAGATCCGCACCCGGGAGA
CCATGGAACATGCGCTGGCTTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCATGCCAACAACGCCAACCAGGCG
CTGGAACGCATCATCCACTTCTTCCCCCACGAGCGGCACGACCAGGTCTGGCTCGACCTGTCGCTCAACCTGCAGGCCAT
CGTCGCCCAGCAGCTGCTGCCGACCCAGGCCGGTGGCCGTTGCCCGGCCATCGAGGTCCTGCTGAAGTCGCCGCTGATCG
CCGACCTGATCCGCAAGGGGGAGGTGGCGGACGTCAAGAACGTCATGGCCCGCTCCCGGGACATGGGCATGCAGACCTTC
GACCAGGCGCTCTATGACCTCTACAAGAAGGGGGCGATCAGCGAAGAGGTCGCGCTGGTGCATGCCGACTCGGCCAACGA
TCTGCGCATGATGATCAAGTACGGCGACGAGCAGGGGGTGGGGCTGGCGCAGGCCAAGGAGGCGGCCAGTCATCTGTCCC
TGCGCGGCGAGGACGACTATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (70-274)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	64.571	92.105	0.595
pilU	Vibrio cholerae strain A1552	60.388	95	0.574
pilU	Acinetobacter baylyi ADP1	57.143	92.105	0.526
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.144	96.579	0.397