Detailed information NTG0235764

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: C6991_RS22410
• Genome accession: NZ_CP027555
• Coordinates: 4051589..4052569 (+)
• Length: 326 a.a.
• NCBI ID: WP_001055634.1
• Uniprot ID: -
• Organism: Escherichia coli strain 2013C-3513
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4046589..4057569

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C6991_RS22380	-	4046968..4047954 (-)	987	WP_000784004.1	TRAP transporter substrate-binding protein	-
C6991_RS22385	hemW	4048266..4049402 (-)	1137	WP_000239919.1	radical SAM family heme chaperone HemW	-
C6991_RS22390	rdgB	4049395..4049988 (-)	594	WP_001174742.1	XTP/dITP diphosphatase	-
C6991_RS22395	yggU	4049996..4050286 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
C6991_RS22400	yggT	4050283..4050849 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
C6991_RS22405	yggS	4050867..4051571 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
C6991_RS22410	pilT	4051589..4052569 (+)	981	WP_001055634.1	type IV pilus twitching motility protein PilT	Machinery gene
C6991_RS22415	ruvX	4052745..4053161 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
C6991_RS22420	yqgE	4053161..4053724 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
C6991_RS22425	gshB	4053833..4054783 (-)	951	WP_000593273.1	glutathione synthase	-
C6991_RS22430	rsmE	4054796..4055527 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
C6991_RS22435	endA	4055607..4056314 (-)	708	WP_000286500.1	deoxyribonuclease I	-
C6991_RS22440	yggI	4056409..4056906 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35942.12 Da
• Isoelectric Point: 5.5860

>NTDB_id=235764 C6991_RS22410 WP_001055634.1 4051589..4052569(+) (pilT) [Escherichia coli strain 2013C-3513]
MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLYQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=235764 C6991_RS22410 WP_001055634.1 4051589..4052569(+) (pilT) [Escherichia coli strain 2013C-3513]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGTCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTAGCGGCGATGGTTGGCTATCTCTATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	48.93	100	0.491
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.93	100	0.491
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	45.872	100	0.46
pilT	Acinetobacter baylyi ADP1	45.872	100	0.46
pilT	Acinetobacter baumannii D1279779	45.872	100	0.46
pilT	Acinetobacter nosocomialis M2	45.872	100	0.46
pilT	Pseudomonas stutzeri DSM 10701	45.872	100	0.46
pilT	Pseudomonas aeruginosa PAK	45.26	100	0.454
pilT	Legionella pneumophila strain ERS1305867	44.343	100	0.445
pilT	Legionella pneumophila strain Lp02	44.343	100	0.445
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.509	97.546	0.405
pilU	Vibrio cholerae strain A1552	40.063	97.239	0.39
pilU	Pseudomonas stutzeri DSM 10701	38.125	98.16	0.374
pilU	Acinetobacter baylyi ADP1	36.728	99.387	0.365
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362