Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CA592_RS14480 Genome accession   NZ_CP021838
Coordinates   2717550..2718584 (+) Length   344 a.a.
NCBI ID   WP_088223690.1    Uniprot ID   A0AAX2A4J8
Organism   Anoxybacillus flavithermus strain 52-1A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2712550..2723584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA592_RS14460 (CA592_14460) - 2712670..2713857 (+) 1188 WP_232467185.1 hypothetical protein -
  CA592_RS14465 (CA592_14465) - 2713854..2714600 (+) 747 WP_088223687.1 PRC-barrel domain-containing protein -
  CA592_RS14470 (CA592_14470) - 2714614..2715876 (+) 1263 WP_088223688.1 VanW family protein -
  CA592_RS14475 (CA592_14475) - 2715873..2717534 (+) 1662 WP_088223689.1 GspE/PulE family protein -
  CA592_RS14480 (CA592_14480) pilT 2717550..2718584 (+) 1035 WP_088223690.1 type IV pilus twitching motility protein PilT Machinery gene
  CA592_RS14485 (CA592_14485) - 2718590..2719801 (+) 1212 WP_088223691.1 type II secretion system F family protein -
  CA592_RS14490 (CA592_14490) - 2719878..2720396 (+) 519 WP_088223692.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CA592_RS14495 (CA592_14495) comC 2720468..2721217 (+) 750 WP_088223693.1 prepilin peptidase Machinery gene
  CA592_RS14500 (CA592_14500) pilM 2721230..2722198 (+) 969 WP_088223694.1 type IV pilus biogenesis protein PilM -
  CA592_RS14505 (CA592_14505) - 2722182..2722763 (+) 582 WP_088223695.1 PilN domain-containing protein -
  CA592_RS14510 (CA592_14510) pilO 2722760..2723386 (+) 627 WP_088223696.1 type 4a pilus biogenesis protein PilO -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37917.90 Da        Isoelectric Point: 7.5472

>NTDB_id=233721 CA592_RS14480 WP_088223690.1 2717550..2718584(+) (pilT) [Anoxybacillus flavithermus strain 52-1A]
MKQKVDAMLRAAFELKASDIHLTVGIPPIFRINGDLKRYGQDVLSPTDTEQMAKAIVPEHMWTRFETDGELDLSYSLSGV
SRFRVNVFKQRGCISLAIRIVPTKIPTLDELQLPDVLKKMVAKPQGLILVTGPTGSGKSTTLAAMIDYMNKTMRKHIITL
EDPIEYVHKHDSCIIDQREVGTDTSSFANGLRAALRQDPDVILVGEMRDLETIQTAVTAAETGHLVFGTLHTSSAPATID
RIIDVFPPEQQGQIRIQLATVLVSIISQRLFPRAQNNGRIAATEILINNAAVANLIRNGKTHQIPSIMQTSRALGMHTLE
TSIKELVQQGLIAKEVVAPYLQEG

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=233721 CA592_RS14480 WP_088223690.1 2717550..2718584(+) (pilT) [Anoxybacillus flavithermus strain 52-1A]
ATGAAACAAAAAGTAGATGCGATGTTGCGTGCTGCATTTGAATTAAAAGCATCCGATATTCATTTAACGGTCGGTATTCC
TCCGATTTTTCGCATTAACGGAGATTTGAAACGATATGGGCAAGATGTATTGTCGCCAACCGATACAGAACAAATGGCAA
AAGCGATCGTTCCAGAGCATATGTGGACGCGCTTTGAAACAGACGGCGAATTAGATTTGTCATATAGTCTTTCAGGCGTT
TCACGTTTTCGTGTGAACGTATTTAAACAACGTGGATGTATATCGTTAGCCATTCGTATCGTACCGACGAAAATTCCAAC
GCTTGATGAACTGCAATTGCCGGACGTATTAAAAAAGATGGTGGCGAAACCACAAGGGCTCATTCTCGTCACGGGGCCGA
CAGGGAGCGGAAAATCGACAACGTTAGCTGCGATGATTGATTATATGAATAAAACGATGCGCAAACATATTATTACGCTT
GAAGATCCGATTGAATATGTGCATAAGCATGACAGCTGCATCATTGATCAGCGCGAAGTCGGAACGGATACGAGCAGCTT
TGCAAACGGGTTGCGCGCTGCTCTTCGCCAAGACCCTGATGTCATTTTAGTCGGGGAAATGCGCGATTTAGAAACGATTC
AAACGGCAGTGACTGCTGCTGAAACGGGGCATCTTGTATTCGGAACGCTTCATACGTCAAGCGCACCAGCAACGATTGAT
CGAATCATTGATGTGTTCCCTCCTGAGCAGCAAGGACAAATTCGTATTCAATTAGCGACCGTTTTAGTTTCAATTATTTC
GCAAAGATTATTTCCGCGCGCCCAAAACAACGGGCGGATTGCCGCGACGGAAATTTTAATAAACAATGCAGCAGTCGCCA
ATTTAATTCGCAACGGAAAAACACATCAAATTCCAAGCATCATGCAGACGAGCCGCGCGCTCGGTATGCATACGCTTGAA
ACGAGCATAAAAGAGCTTGTTCAGCAAGGTTTGATCGCTAAAGAAGTCGTTGCACCGTACTTGCAGGAAGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.462

94.477

0.515
  pilT Vibrio cholerae strain A1552

50.453

96.221

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.453

96.221

0.485
  pilT Pseudomonas aeruginosa PAK

50.613

94.767

0.48
  pilT Neisseria meningitidis 8013

50.779

93.314

0.474
  pilT Neisseria gonorrhoeae MS11

50.313

93.023

0.468
  pilT Pseudomonas stutzeri DSM 10701

49.08

94.767

0.465
  pilT Acinetobacter baumannii D1279779

49.074

94.186

0.462
  pilT Acinetobacter nosocomialis M2

49.074

94.186

0.462
  pilT Acinetobacter baumannii strain A118

49.074

94.186

0.462
  pilT Acinetobacter baylyi ADP1

48.615

94.477

0.459
  pilT Legionella pneumophila strain Lp02

47.649

92.733

0.442
  pilT Legionella pneumophila strain ERS1305867

47.649

92.733

0.442
  pilU Pseudomonas stutzeri DSM 10701

42.771

96.512

0.413
  pilU Vibrio cholerae strain A1552

40.979

95.058

0.39
  pilU Acinetobacter baylyi ADP1

40.752

92.733

0.378

Multiple sequence alignment