Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CDA09_RS18800 Genome accession   NZ_CP021731
Coordinates   4065457..4066497 (+) Length   346 a.a.
NCBI ID   WP_121430043.1    Uniprot ID   -
Organism   Azoarcus sp. DN11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4060457..4071497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDA09_RS18770 (CDA09_18745) - 4060553..4061530 (+) 978 WP_121430038.1 aspartate carbamoyltransferase catalytic subunit -
  CDA09_RS18775 (CDA09_18750) - 4061527..4062804 (+) 1278 WP_121430039.1 dihydroorotase -
  CDA09_RS18780 (CDA09_18755) - 4062880..4063236 (-) 357 WP_121430040.1 DUF167 family protein -
  CDA09_RS18785 (CDA09_18760) - 4063233..4063811 (-) 579 WP_121430041.1 YggT family protein -
  CDA09_RS18790 (CDA09_18765) proC 4063817..4064629 (-) 813 WP_121430935.1 pyrroline-5-carboxylate reductase -
  CDA09_RS18795 (CDA09_18770) - 4064653..4065357 (-) 705 WP_121430042.1 YggS family pyridoxal phosphate-dependent enzyme -
  CDA09_RS18800 (CDA09_18775) pilT 4065457..4066497 (+) 1041 WP_121430043.1 type IV pilus twitching motility protein PilT Machinery gene
  CDA09_RS18805 (CDA09_18780) pilU 4066635..4067768 (+) 1134 WP_121430044.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CDA09_RS18810 (CDA09_18785) - 4068003..4069163 (-) 1161 WP_121430045.1 THUMP domain-containing protein -
  CDA09_RS18815 (CDA09_18790) - 4069245..4069655 (-) 411 WP_121430046.1 CopD family protein -
  CDA09_RS18820 (CDA09_18795) - 4069655..4070557 (-) 903 WP_121430047.1 NAD-dependent epimerase/dehydratase family protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38567.28 Da        Isoelectric Point: 7.0222

>NTDB_id=232926 CDA09_RS18800 WP_121430043.1 4065457..4066497(+) (pilT) [Azoarcus sp. DN11]
MDITELLAFAVKNKASDLHLSSGLPPMIRVHGDVRRINLPPMEHKDVHSMVYDIMNDQQRKQYEEVLECDFSFAVPNLAR
FRVNAFNQNRGAAAVFRTIPSKVLTLEELNCPKIFKDISNQPRGIVLVTGPTGSGKSTTLAAMIDHINDNEYGHILTIED
PIEFVHESKRSLINQREIARDTLSFNNALRSALREDPDVILVGEMRDLETIRLALTGAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQNVGMQTLDQC
LLDLVRRNIVSPAEAKMKAQNKDNFG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=232926 CDA09_RS18800 WP_121430043.1 4065457..4066497(+) (pilT) [Azoarcus sp. DN11]
ATGGACATCACCGAACTGCTCGCCTTCGCAGTCAAGAACAAGGCCTCCGACCTCCATCTCTCGTCCGGTCTGCCACCCAT
GATCCGGGTGCACGGCGACGTGCGGCGCATCAACCTGCCGCCAATGGAGCACAAGGACGTGCACTCCATGGTGTACGACA
TCATGAACGACCAGCAGCGCAAGCAATACGAGGAAGTGCTGGAGTGCGACTTCTCGTTCGCGGTGCCCAACCTCGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGCCGCCGTGTTCCGGACCATCCCGTCCAAGGTGCTGACGCTCGA
GGAGCTCAACTGTCCGAAGATCTTCAAGGACATCTCGAACCAGCCGCGCGGCATCGTGCTCGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACGACGCTCGCGGCGATGATCGATCACATCAATGACAACGAATACGGCCATATCCTCACCATCGAGGAC
CCGATCGAATTCGTGCACGAGTCGAAGCGCAGCCTGATCAACCAGCGCGAGATCGCACGCGATACGCTGTCGTTCAATAA
CGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACGTGATCCTCGTCGGCGAAATGCGCGACCTCGAAACCATCCGCCTCG
CGCTGACCGGCGCCGAGACCGGCCACCTCGTGTTCGGCACGCTGCACACCTCGTCCGCCGCCAAGACCATCGACCGTATC
GTCGACGTCTTCCCGGCGGCCGAGAAGGAGATGGTCCGCTCGATGCTGTCGGAGTCGCTGCGCGCGGTGATCTCGCAGAC
CCTGCTGAAGACCAAGGACGGCCAGGGCCGCGTCGCCGCCCACGAGATCATGATCGGCACCCCCGCCATCCGCAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGTCGATCCAGACCGGCCAGAACGTCGGCATGCAGACGCTCGACCAGTGC
CTGCTCGACCTCGTGCGGCGCAACATCGTCTCGCCCGCCGAGGCCAAGATGAAGGCCCAGAACAAGGACAATTTCGGCTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.971

99.711

0.777
  pilT Acinetobacter baumannii D1279779

77.971

99.711

0.777
  pilT Acinetobacter baumannii strain A118

77.971

99.711

0.777
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.711

0.766
  pilT Pseudomonas aeruginosa PAK

76.522

99.711

0.763
  pilT Acinetobacter baylyi ADP1

75.942

99.711

0.757
  pilT Legionella pneumophila strain Lp02

73.043

99.711

0.728
  pilT Legionella pneumophila strain ERS1305867

73.043

99.711

0.728
  pilT Neisseria meningitidis 8013

72.464

99.711

0.723
  pilT Neisseria gonorrhoeae MS11

72.174

99.711

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.059

98.266

0.659
  pilT Vibrio cholerae strain A1552

67.059

98.266

0.659
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.985

95.665

0.526
  pilU Acinetobacter baylyi ADP1

41.092

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

40

99.711

0.399
  pilU Vibrio cholerae strain A1552

39.71

99.711

0.396

Multiple sequence alignment