Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CDA09_RS18805 Genome accession   NZ_CP021731
Coordinates   4066635..4067768 (+) Length   377 a.a.
NCBI ID   WP_121430044.1    Uniprot ID   -
Organism   Azoarcus sp. DN11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4061635..4072768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDA09_RS18780 (CDA09_18755) - 4062880..4063236 (-) 357 WP_121430040.1 DUF167 family protein -
  CDA09_RS18785 (CDA09_18760) - 4063233..4063811 (-) 579 WP_121430041.1 YggT family protein -
  CDA09_RS18790 (CDA09_18765) proC 4063817..4064629 (-) 813 WP_121430935.1 pyrroline-5-carboxylate reductase -
  CDA09_RS18795 (CDA09_18770) - 4064653..4065357 (-) 705 WP_121430042.1 YggS family pyridoxal phosphate-dependent enzyme -
  CDA09_RS18800 (CDA09_18775) pilT 4065457..4066497 (+) 1041 WP_121430043.1 type IV pilus twitching motility protein PilT Machinery gene
  CDA09_RS18805 (CDA09_18780) pilU 4066635..4067768 (+) 1134 WP_121430044.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CDA09_RS18810 (CDA09_18785) - 4068003..4069163 (-) 1161 WP_121430045.1 THUMP domain-containing protein -
  CDA09_RS18815 (CDA09_18790) - 4069245..4069655 (-) 411 WP_121430046.1 CopD family protein -
  CDA09_RS18820 (CDA09_18795) - 4069655..4070557 (-) 903 WP_121430047.1 NAD-dependent epimerase/dehydratase family protein -
  CDA09_RS18825 (CDA09_18800) - 4070579..4071601 (+) 1023 WP_121430048.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  CDA09_RS18830 (CDA09_18805) - 4071606..4072742 (+) 1137 WP_121430049.1 HDOD domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42200.60 Da        Isoelectric Point: 6.5062

>NTDB_id=232927 CDA09_RS18805 WP_121430044.1 4066635..4067768(+) (pilU) [Azoarcus sp. DN11]
MERDQALKFMHDLLRLMVQKNGSDLFITAGFPPAIKIDGRVIPQSNQSLTQQHTAELARAVMNDRQAAEFESSKECNFAI
SPAGIGRFRANAFIQQGKVGLVLRTIAQKIPTFDELGMPAVLRDVAMAKRGLVIFVGGTGTGKTTSLAAMVDFRNEHSYG
HIITVEDPIEYVHLHKNCIVTQREIGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLRSLISQRLLPKKAEKGRVPAVEVLLNSPLISDLIFKGEVPGIKEVMKRSRELG
MQTFDQALFDLYEDEKITYEDALRNADSVNDLRLQIKLHSKLGEKDLVSGIQHLDIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=232927 CDA09_RS18805 WP_121430044.1 4066635..4067768(+) (pilU) [Azoarcus sp. DN11]
ATGGAACGCGATCAGGCCCTCAAGTTCATGCACGACCTGCTCAGGCTGATGGTGCAGAAGAACGGCTCCGACCTCTTCAT
CACCGCCGGCTTCCCGCCCGCGATCAAGATCGACGGGCGCGTCATCCCGCAGTCCAACCAGTCGCTCACGCAGCAGCACA
CCGCCGAACTCGCGCGCGCCGTGATGAACGACCGCCAGGCGGCGGAGTTCGAATCCAGCAAGGAATGCAACTTCGCGATC
TCGCCCGCGGGCATCGGCCGCTTCCGTGCCAACGCCTTCATCCAGCAGGGCAAGGTCGGCCTCGTGCTGCGAACCATCGC
GCAGAAGATCCCGACCTTCGACGAGCTGGGCATGCCGGCGGTGCTGCGCGACGTCGCGATGGCCAAGCGCGGCCTCGTGA
TCTTCGTCGGCGGCACCGGCACCGGCAAGACGACCTCGCTCGCCGCGATGGTCGACTTCCGCAACGAGCACTCCTACGGC
CACATCATCACGGTCGAGGACCCGATCGAATACGTGCATCTGCACAAGAACTGCATCGTCACGCAACGCGAGATCGGGCT
CGACACCGACAGCTGGGAAGCGGCGCTGAAAAACACCCTGCGCCAGGCCCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACGATGGACTACGCGATTGCGTTCGCCGAAACCGGCCACCTGTGTCTCGCCACACTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCGGAAGAACGCCGCCAGCAGCTGCTGATGGATCTGTCGCTCAACCT
GCGCTCGCTGATCTCGCAGCGCCTGCTGCCGAAGAAAGCCGAGAAGGGACGCGTCCCGGCGGTGGAAGTGCTGCTCAACT
CGCCGCTGATCTCGGACCTGATCTTCAAGGGCGAGGTGCCGGGCATCAAGGAAGTGATGAAGCGCTCGCGCGAGCTCGGG
ATGCAGACCTTCGACCAGGCGCTGTTCGATCTCTACGAGGACGAGAAGATCACCTACGAGGACGCGCTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCCTGCAGATCAAGCTCCACAGCAAGCTCGGCGAGAAGGACCTGGTGTCCGGCATCCAGCACC
TCGACATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.813

96.552

0.597
  pilU Acinetobacter baylyi ADP1

59.143

92.838

0.549
  pilU Vibrio cholerae strain A1552

55.278

95.491

0.528
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.179

88.859

0.393
  pilT Pseudomonas aeruginosa PAK

42.388

88.859

0.377
  pilT Pseudomonas stutzeri DSM 10701

41.194

88.859

0.366
  pilT Legionella pneumophila strain Lp02

41.39

87.798

0.363
  pilT Legionella pneumophila strain ERS1305867

41.39

87.798

0.363
  pilT Acinetobacter baylyi ADP1

42.812

84.881

0.363

Multiple sequence alignment