Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CCX87_RS02005 Genome accession   NZ_CP021648
Coordinates   420095..421138 (-) Length   347 a.a.
NCBI ID   WP_087748127.1    Uniprot ID   -
Organism   Acidovorax sp. T1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415095..426138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCX87_RS01975 - 415393..415791 (+) 399 WP_232476461.1 YraN family protein -
  CCX87_RS01980 - 415879..416478 (+) 600 WP_087743337.1 SIS domain-containing protein -
  CCX87_RS01985 - 416475..417134 (+) 660 WP_087743339.1 BON domain-containing protein -
  CCX87_RS01990 - 417195..418109 (-) 915 WP_087743342.1 NAD(P)-dependent oxidoreductase -
  CCX87_RS01995 pilU 418213..419349 (-) 1137 WP_087743344.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CCX87_RS02000 - 419390..420025 (-) 636 WP_087743345.1 cyclic nucleotide-binding domain-containing protein -
  CCX87_RS02005 pilT 420095..421138 (-) 1044 WP_087748127.1 type IV pilus twitching motility protein PilT Machinery gene
  CCX87_RS02010 - 421176..421892 (+) 717 WP_087743347.1 YggS family pyridoxal phosphate-dependent enzyme -
  CCX87_RS02015 - 422039..423178 (+) 1140 WP_087743349.1 aminotransferase class V-fold PLP-dependent enzyme -
  CCX87_RS02020 - 423242..424549 (+) 1308 WP_087748128.1 DUF445 domain-containing protein -
  CCX87_RS02025 queA 424698..425849 (-) 1152 WP_087743351.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38208.98 Da        Isoelectric Point: 6.7743

>NTDB_id=232244 CCX87_RS02005 WP_087748127.1 420095..421138(-) (pilT) [Acidovorax sp. T1]
MDITQLLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDSQRKTYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRSMLSESLQAVISQTLCKLKDGSGRVAAHEIMLGTSAIRNLIRESKVAQMYSTIQTGNSLGMQTLDQN
LTDLVRRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=232244 CCX87_RS02005 WP_087748127.1 420095..421138(-) (pilT) [Acidovorax sp. T1]
GTGGACATTACCCAGTTGCTCGCATTCAGCGTGAAGAACAAAGCTTCCGACCTGCACTTGTCCTCCGGGTTGCCGCCCAT
GATCCGGGTCCACGGCGATGTGCGGCGCATCAATGTGGATGCGCTGGACCACAAGACGGTGCACGCCATGGTGTACGACA
TCATGAGCGACTCGCAGCGCAAGACGTATGAAGAGTTTCTGGAGGTGGACTTCTCGTTTGAGATCGAAGGCCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCAGAACCGCGGCGCGGCCGCCGTGTTCCGCACCATTCCAAGCAAGATCCTCACGCTGGA
ACAGCTCAACGCCCCCAAGATCTTCGGCGACCTGGCCCTCAAACCGCGCGGCCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCGGCCATGGTGAATTATCTGAACGAAACCGAATACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGGCCCATGACGCTGTCGTTCGCGGC
AGCCCTCAAATCCGCGCTGCGCGAAGACCCCGACGCCATTTTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACCGCCGCCGAAACCGGCCACCTGGTGTTTGGCACGCTGCACACATCCAGCGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCCGCCGAAGAAAAGGAAATGGTGCGCTCCATGCTGTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
GCTGTGCAAGCTCAAGGACGGCTCGGGCCGGGTGGCCGCGCACGAAATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGTCCAAGGTGGCGCAGATGTATTCCACCATCCAGACCGGCAACAGCCTGGGCATGCAGACGCTGGACCAGAAC
CTCACCGATCTGGTGCGGCGCAACATCATCAGCCCCGCTGAAGCCCGCAGCAAGGCCAAGATTCCCGAAAATTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

77.391

99.424

0.769
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Neisseria meningitidis 8013

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.742

98.271

0.666
  pilT Vibrio cholerae strain A1552

67.742

98.271

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Vibrio cholerae strain A1552

43.582

96.542

0.421
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment