Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CCX87_RS01995 Genome accession   NZ_CP021648
Coordinates   418213..419349 (-) Length   378 a.a.
NCBI ID   WP_087743344.1    Uniprot ID   -
Organism   Acidovorax sp. T1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 413213..424349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCX87_RS01965 - 413390..414325 (+) 936 WP_087743334.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  CCX87_RS01970 rsmI 414446..415375 (-) 930 WP_198314752.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  CCX87_RS01975 - 415393..415791 (+) 399 WP_232476461.1 YraN family protein -
  CCX87_RS01980 - 415879..416478 (+) 600 WP_087743337.1 SIS domain-containing protein -
  CCX87_RS01985 - 416475..417134 (+) 660 WP_087743339.1 BON domain-containing protein -
  CCX87_RS01990 - 417195..418109 (-) 915 WP_087743342.1 NAD(P)-dependent oxidoreductase -
  CCX87_RS01995 pilU 418213..419349 (-) 1137 WP_087743344.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CCX87_RS02000 - 419390..420025 (-) 636 WP_087743345.1 cyclic nucleotide-binding domain-containing protein -
  CCX87_RS02005 pilT 420095..421138 (-) 1044 WP_087748127.1 type IV pilus twitching motility protein PilT Machinery gene
  CCX87_RS02010 - 421176..421892 (+) 717 WP_087743347.1 YggS family pyridoxal phosphate-dependent enzyme -
  CCX87_RS02015 - 422039..423178 (+) 1140 WP_087743349.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41757.11 Da        Isoelectric Point: 6.9134

>NTDB_id=232243 CCX87_RS01995 WP_087743344.1 418213..419349(-) (pilU) [Acidovorax sp. T1]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKVDGKVTKVSPQPLTPNHTLTLARAIMSDKQVADFERTKESNFAI
SPAGIGRFRVNAFIQQGKVGMVLRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEVMKKSRNLG
MQTFDQALFDAYEANVISYEDALRNADSLNDLRLQIKLNSQRAKSPDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=232243 CCX87_RS01995 WP_087743344.1 418213..419349(-) (pilU) [Acidovorax sp. T1]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGATCTGCTCAAGCTGATGGTGAGCCGCAATGGCAGCGACTTGTTCAT
CACGGCAGAGTTTCCGCCCGCCATCAAGGTCGATGGCAAGGTGACCAAGGTATCGCCCCAGCCGCTCACGCCCAACCACA
CCCTGACGCTGGCCCGCGCCATCATGAGCGACAAGCAGGTGGCAGACTTCGAGCGCACCAAGGAATCCAATTTCGCCATC
TCACCGGCCGGCATCGGGCGCTTTCGCGTGAACGCATTCATCCAGCAGGGCAAGGTCGGCATGGTGCTGCGCACGATTCC
GCTCACGTTGCCCACCATTGACGGTCTGGGCGTGCCGCAGGTCCTCAAGGAAGTGACGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCCACCACGCTCGCGGCCATGGTGGACTGGCGCAACGAAAACTCGTTCGGC
CACATCATCACGGTGGAAGACCCGGTCGAATTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGGCT
CGATACCGACAGCTGGGAAGCGGCCCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAACATGCCGTGGCCTTTGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCGCCCAACTGCTGATGGACCTGTCGCTCAACCT
GCGCGCCATGGTGTCGCAGCGCCTCATCCCCAAGCAGGACGGCAAGGGCCGGGCTGCGGCGGTGGAGGTGATGCTCAACA
CCCCGCTGATCGCCGACCTGATCTTCAAGGGCGAAGTCGCCGAGATCAAGGAAGTCATGAAAAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGATGCCTACGAGGCCAATGTGATCAGCTACGAAGATGCGCTACGCAACGCCGA
CTCGCTCAACGACCTGCGTCTGCAGATCAAGCTCAACAGCCAGCGCGCCAAGTCGCCCGACCTGGCCTCGGGCACCGAGC
ATTTCGCGATTGTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.183

92.328

0.593
  pilU Acinetobacter baylyi ADP1

57.983

94.444

0.548
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.153

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Acinetobacter baylyi ADP1

43.574

84.392

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362

Multiple sequence alignment