Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CCO03_RS15580 Genome accession   NZ_CP021455
Coordinates   3655142..3656185 (+) Length   347 a.a.
NCBI ID   WP_087282521.1    Uniprot ID   A0A1Y0EQG9
Organism   Comamonas serinivorans strain DSM 26136     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3650142..3661185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCO03_RS15555 (CCO03_15555) - 3650198..3650467 (+) 270 WP_087282510.1 DUF3567 domain-containing protein -
  CCO03_RS15560 (CCO03_15560) pyrC 3650840..3651880 (+) 1041 WP_087282512.1 dihydroorotase -
  CCO03_RS15565 (CCO03_15565) - 3652019..3652864 (+) 846 WP_087282514.1 NYN domain-containing protein -
  CCO03_RS15570 (CCO03_15570) htpX 3653202..3654083 (+) 882 WP_087282516.1 protease HtpX -
  CCO03_RS15575 (CCO03_15575) - 3654272..3654973 (+) 702 WP_087282519.1 MarC family protein -
  CCO03_RS15580 (CCO03_15580) pilT 3655142..3656185 (+) 1044 WP_087282521.1 type IV pilus twitching motility protein PilT Machinery gene
  CCO03_RS15585 (CCO03_15585) pilU 3656262..3657398 (+) 1137 WP_087282524.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CCO03_RS15590 (CCO03_15590) - 3657814..3659595 (-) 1782 WP_087282526.1 acyclic terpene utilization AtuA family protein -
  CCO03_RS15595 (CCO03_15595) - 3659688..3660455 (-) 768 WP_087282528.1 enoyl-CoA hydratase/isomerase family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38281.94 Da        Isoelectric Point: 6.7244

>NTDB_id=230869 CCO03_RS15580 WP_087282521.1 3655142..3656185(+) (pilT) [Comamonas serinivorans strain DSM 26136]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRLNVEPLDHKQVFAMVYDIMNDAQRKAYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAGAVLRTIPSKILSLEQLNAPKIFGELSLQPRGLVLVTGPTGSGKSTTLAAMINHLNESEYAHVLTVED
PIEFVHESKKCLINQREVGPHTLSFTNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRSMLSESLNAVIAQTLCKTKDGQGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGHAYGMQTLDQC
LSDLVRRNAISKAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=230869 CCO03_RS15580 WP_087282521.1 3655142..3656185(+) (pilT) [Comamonas serinivorans strain DSM 26136]
GTGGACATCACCCAACTGCTGGCGTTCAGCGTCAAGAACAAGGCGTCTGACCTGCACCTGTCAGCCGGGTTGCCCCCCAT
GATTCGCGTGCACGGCGACGTGCGTCGCCTGAACGTGGAACCGTTGGACCACAAACAGGTGTTCGCCATGGTGTACGACA
TCATGAACGACGCCCAGCGCAAGGCGTACGAAGAATTCCTGGAGGTGGACTTCTCGTTCGAAATCGACGGCCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCAGAACCGGGGCGCCGGCGCCGTGCTGCGGACCATCCCGAGCAAGATCCTGTCGCTGGA
GCAGCTCAACGCGCCCAAGATCTTCGGCGAGCTGTCGCTGCAGCCGCGGGGCCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCGGCCATGATCAACCACCTCAACGAAAGCGAGTACGCCCACGTGCTGACGGTGGAAGAC
CCCATCGAGTTCGTGCACGAGTCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGCACACGCTGAGCTTCACCAA
CGCCCTGCGGTCGGCCCTGCGCGAAGACCCCGACGCCATCCTCGTGGGCGAATTGCGCGACCTGGAGACGATTCGCCTGG
CCATGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCAGCCGAGGAAAAGGAAATGGTGCGCTCCATGCTGTCCGAGTCGCTGAACGCCGTGATTGCCCAGAC
CCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCCGCGCACGAGATCATGATCGGCACCCCGGCCATCCGCAACCTGA
TCCGCGAGGACAAGGTGGCTCAGATGTACTCATCCATCCAGACCGGCCATGCCTACGGCATGCAGACGCTGGACCAATGC
CTGTCCGACCTGGTGCGGCGCAACGCGATCAGCAAGGCTGAGGCCCGTGGCAAGGCCAAGATTCCGGAGAACTTCCCCGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0EQG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

74.493

99.424

0.741
  pilT Acinetobacter baumannii D1279779

74.493

99.424

0.741
  pilT Acinetobacter baumannii strain A118

74.493

99.424

0.741
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

75.221

97.695

0.735
  pilT Legionella pneumophila strain ERS1305867

75.221

97.695

0.735
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.742

98.271

0.666
  pilT Vibrio cholerae strain A1552

67.742

98.271

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Vibrio cholerae strain A1552

42.687

96.542

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406

Multiple sequence alignment