Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AKG35_RS00095 Genome accession   NC_005071
Coordinates   21943..23019 (-) Length   358 a.a.
NCBI ID   WP_011129398.1    Uniprot ID   A2C5L5
Organism   Prochlorococcus marinus str. MIT 9313     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16943..28019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKG35_RS00070 (PMT0014) - 17233..17832 (+) 600 WP_011129393.1 RNA recognition motif domain-containing protein -
  AKG35_RS00075 (PMT0015) dusA 17842..18846 (-) 1005 WP_041384817.1 tRNA dihydrouridine(20/20a) synthase DusA -
  AKG35_RS00080 (PMT0016) msrB 18916..19422 (+) 507 WP_011129395.1 peptide-methionine (R)-S-oxide reductase MsrB -
  AKG35_RS00085 (PMT0017) - 19349..20671 (+) 1323 WP_011129396.1 NAD(P)/FAD-dependent oxidoreductase -
  AKG35_RS00090 (PMT0018) - 20646..21926 (-) 1281 WP_011129397.1 type II secretion system F family protein -
  AKG35_RS00095 (PMT0019) pilT 21943..23019 (-) 1077 WP_011129398.1 type IV pilus twitching motility protein PilT Machinery gene
  AKG35_RS00100 (PMT0020) pilB/pilB1 23030..24946 (-) 1917 WP_011129399.1 GspE/PulE family protein Machinery gene
  AKG35_RS00105 (PMT0021) grpE 24945..25658 (+) 714 WP_011129400.1 nucleotide exchange factor GrpE -
  AKG35_RS00110 (PMT0022) dnaJ 25703..26839 (+) 1137 WP_011129401.1 molecular chaperone DnaJ -
  AKG35_RS00115 (PMT0023) - 26836..27081 (+) 246 WP_011129402.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39379.87 Da        Isoelectric Point: 5.9277

>NTDB_id=23035 AKG35_RS00095 WP_011129398.1 21943..23019(-) (pilT) [Prochlorococcus marinus str. MIT 9313]
MDLMIEDLMEQLVQGGGSDLHIATGQPPYGRFSGELRPMTNTPLSEETCNKLIFSMLNNSQRKTLEQIWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIESLNLPSVVIETSKRPRGLVLVTGPTGSGKTTTLASLLDHINHTRSEHILTI
EDPIEFVYKSDLSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTNSAAQTVD
RMVDVFPPNQQTQIRVQLSGSLLAIFSQTLCRRLNPQPEQFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGELGM
QTLEKALANLTKQGEISRSEAIAKASKPSELEQLLNDG

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=23035 AKG35_RS00095 WP_011129398.1 21943..23019(-) (pilT) [Prochlorococcus marinus str. MIT 9313]
ATGGATCTAATGATCGAGGACCTGATGGAACAGCTGGTGCAAGGTGGCGGCAGTGATCTGCACATTGCTACCGGCCAACC
CCCGTATGGACGATTCAGCGGTGAATTGAGGCCAATGACCAATACCCCCCTATCCGAAGAAACATGCAACAAGCTGATTT
TCTCAATGCTCAACAACAGTCAGAGAAAGACTCTTGAACAAATCTGGGAGCTTGACTGTGCCTATGGGCTAAAGGGTGTG
GCGCGCTTTCGTGTGAATGTCTATCGACAGAAGGGAAGTTACGCCGCCTGTCTACGTGCTCTTGGCAGCACTATCCCGAG
TATTGAAAGTCTCAACCTGCCATCAGTAGTCATAGAAACCAGCAAACGACCAAGGGGATTAGTGCTAGTAACAGGACCAA
CCGGATCAGGCAAAACAACAACCCTTGCTTCCCTGCTGGACCACATCAATCACACCAGAAGCGAACACATCCTCACAATC
GAAGATCCAATCGAATTTGTATACAAAAGCGATCTCAGCCTTGTGCACCAGCGACAGCTCAATGAAGACACACGCAGTTT
TGGCAATGCCCTGCGTGCGGCACTACGCGAAGATCCAGATGTGATCCTCGTGGGCGAAATGCGCGACCTGGAAACCATTC
AACTGGCCATCAGCGCCGCCGAAACAGGTCACCTCGTATTCGGCACCCTGCACACCAACTCGGCGGCACAAACGGTCGAT
CGCATGGTTGATGTTTTCCCCCCAAACCAGCAAACCCAAATCAGAGTTCAACTCTCAGGAAGCCTGCTAGCCATTTTTTC
TCAAACCCTCTGCCGGCGCCTAAATCCACAACCAGAACAATTTGGGCGGGTCATGGCTCAAGAAATCTTGATCAACACGC
CAGCCACAGCAAACCTGATTAGAGAAGGCAAAACCGCACAGCTCTACTCCCAAATTCAAACCGGAGGAGAACTAGGGATG
CAAACACTAGAAAAAGCCCTTGCGAATCTAACTAAACAGGGTGAAATTAGCCGCTCTGAAGCCATCGCCAAAGCCAGTAA
ACCCAGCGAACTGGAGCAACTCCTCAACGATGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2C5L5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.907

96.089

0.508
  pilT Pseudomonas stutzeri DSM 10701

52.326

96.089

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.858

98.603

0.492
  pilT Neisseria gonorrhoeae MS11

49.713

97.207

0.483
  pilT Acinetobacter baylyi ADP1

50.291

96.089

0.483
  pilT Legionella pneumophila strain Lp02

51.335

94.134

0.483
  pilT Legionella pneumophila strain ERS1305867

51.335

94.134

0.483
  pilT Neisseria meningitidis 8013

50.146

95.81

0.48
  pilT Acinetobacter baumannii strain A118

49.28

96.927

0.478
  pilT Acinetobacter baumannii D1279779

49.28

96.927

0.478
  pilT Acinetobacter nosocomialis M2

49.28

96.927

0.478
  pilT Vibrio cholerae strain A1552

48.235

94.972

0.458
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.235

94.972

0.458
  pilU Pseudomonas stutzeri DSM 10701

40.395

98.883

0.399
  pilU Acinetobacter baylyi ADP1

37.644

97.207

0.366
  pilU Vibrio cholerae strain A1552

37.941

94.972

0.36

Multiple sequence alignment