Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AKG35_RS00100 Genome accession   NC_005071
Coordinates   23030..24946 (-) Length   638 a.a.
NCBI ID   WP_011129399.1    Uniprot ID   Q7V9D0
Organism   Prochlorococcus marinus str. MIT 9313     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 18030..29946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKG35_RS00080 (PMT0016) msrB 18916..19422 (+) 507 WP_011129395.1 peptide-methionine (R)-S-oxide reductase MsrB -
  AKG35_RS00085 (PMT0017) - 19349..20671 (+) 1323 WP_011129396.1 NAD(P)/FAD-dependent oxidoreductase -
  AKG35_RS00090 (PMT0018) - 20646..21926 (-) 1281 WP_011129397.1 type II secretion system F family protein -
  AKG35_RS00095 (PMT0019) pilT 21943..23019 (-) 1077 WP_011129398.1 type IV pilus twitching motility protein PilT Machinery gene
  AKG35_RS00100 (PMT0020) pilB/pilB1 23030..24946 (-) 1917 WP_011129399.1 GspE/PulE family protein Machinery gene
  AKG35_RS00105 (PMT0021) grpE 24945..25658 (+) 714 WP_011129400.1 nucleotide exchange factor GrpE -
  AKG35_RS00110 (PMT0022) dnaJ 25703..26839 (+) 1137 WP_011129401.1 molecular chaperone DnaJ -
  AKG35_RS00115 (PMT0023) - 26836..27081 (+) 246 WP_011129402.1 sulfurtransferase TusA family protein -
  AKG35_RS00120 (PMT0024) rsgA 27068..28033 (+) 966 WP_011129403.1 ribosome small subunit-dependent GTPase A -
  AKG35_RS00125 (PMT0025) - 27990..28331 (-) 342 WP_011129404.1 YbaB/EbfC family nucleoid-associated protein -
  AKG35_RS00130 (PMT0026) murB 28356..29279 (-) 924 WP_011129405.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 638 a.a.        Molecular weight: 70505.47 Da        Isoelectric Point: 6.4570

>NTDB_id=23036 AKG35_RS00100 WP_011129399.1 23030..24946(-) (pilB/pilB1) [Prochlorococcus marinus str. MIT 9313]
MLTVCKFTSRHVDGNSTPPTSGGSPHLQFHFFFVTQGRPIPKATNSIQQRLELELLLQVNVLSHEELVVGVELMANHTTL
DISTWQQFQALPINMHNQHLVVAISDQCNEQTKNQLISVLQSQGFSTEFRLALAADISQLLAPMRSEQHVESASKSKTTK
PIAQTPTSLLAGFSAEGVLEEDPEEQARLASSIEDLESSLMDSDSSPVINLVDRILLEALQTEASDVHVEPQQDGLQIRF
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSLPSRYGEKICLRLLDSSAPQL
GLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFD
FSTALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPKCQGSGYKGRVGVYEVLRMNEELASAVAKGATTD
LVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDSGLESERRARALSTLTCNVCGAGLQDDWLECPYCLTARQ

Nucleotide


Download         Length: 1917 bp        

>NTDB_id=23036 AKG35_RS00100 WP_011129399.1 23030..24946(-) (pilB/pilB1) [Prochlorococcus marinus str. MIT 9313]
ATGCTGACCGTTTGCAAATTCACGTCTAGACATGTTGATGGGAATAGCACACCTCCCACAAGCGGCGGAAGCCCGCACCT
CCAATTTCATTTCTTCTTCGTGACCCAGGGCCGACCAATCCCCAAAGCAACCAACAGCATCCAGCAGCGACTGGAACTGG
AACTGCTCCTACAAGTCAATGTGCTTAGCCACGAAGAACTTGTTGTCGGAGTGGAGCTAATGGCCAACCACACCACTCTT
GACATCTCAACGTGGCAACAATTCCAGGCCCTGCCAATCAACATGCACAACCAACATTTGGTTGTAGCCATCTCGGACCA
ATGCAATGAGCAAACCAAAAATCAACTGATCTCAGTGCTGCAGTCCCAAGGCTTCAGCACAGAATTCCGTCTCGCGCTTG
CAGCCGACATCAGCCAGCTGCTTGCACCAATGAGATCTGAGCAGCACGTTGAATCTGCATCCAAGTCAAAAACAACAAAG
CCAATTGCACAAACACCAACCTCCCTGCTGGCAGGATTTAGTGCCGAAGGCGTGCTCGAAGAAGATCCTGAAGAACAAGC
CAGACTTGCTAGTTCCATAGAGGATTTGGAGTCCAGCTTGATGGACTCAGACAGTTCACCAGTCATCAACCTGGTGGACC
GCATATTGCTAGAGGCACTGCAAACAGAAGCCAGCGACGTACACGTTGAGCCGCAACAAGACGGACTACAGATCCGCTTC
CGTCAAGACGGTGTCCTGCAGCGCTATATCGAACCCCTCCCGAGCCGGTTGATCCCTGCTGTTACCTCACGCTTCAAGAT
CATGGCCGACCTAGACATCGCAGAACGGCGCATGGCTCAGGACGGTCGCATTCGTCGCACATATCGCAATCGTATGGTCG
ATTTCAGGGTTAATAGCCTGCCAAGCCGTTACGGGGAAAAAATCTGTCTGCGACTACTCGACAGCAGTGCTCCACAACTC
GGGTTAGACAAACTGATCAGCAACCCAAGTGCTCTCTCTCTCGTACGCAACTTGGGCTCTAAGCCCTTCGGCATGATCCT
TGTGACAGGTCCAACAGGGTCAGGCAAGTCAACAACCCTTTACTCCCTCCTGGCAGAACGCAACGAACCTGGTATCAACA
TTTCCACCGTAGAAGATCCCATCGAATACACCCTCCCTGGGATTACCCAATGTCAAGTGAATAGGGAAAAAGGCTTCGAT
TTCAGCACTGCACTGAGAGCCTTCATGCGTCAAGACCCAGACGTTCTGCTGGTCGGTGAAACCCGTGACCTAGAAACCGC
AAAAACCGCCATCGAAGCCGCACTGACTGGTCATCTGGTACTAACCACACTGCATTGCAATGACGCATCAAGTGCGATCG
CCCGCCTCAACGAGATGGGCGTGGAACCATTCATGGTGAGCGCTTCGTTGATTGGCATCGTCTCTCAACGACTTCTACGA
AGAGTGTGCCGTTCTTGTCGTAAGTCATATCACCCAACTGAAAAAGAACTGGGGCGATTCGGCTTGATGGCCCATACAGA
AACTGGGGTGACCTTTTTCAAGGCCCACCATCACGGTCAAGAAAAACAGCCGTGCCCCAAATGTCAAGGCAGCGGCTACA
AGGGCCGAGTTGGTGTCTATGAAGTACTGCGCATGAACGAAGAGCTCGCTTCAGCCGTCGCCAAAGGTGCCACTACTGAT
TTAGTAAGACGTCTGGCGCTCGAGGCTGGTATGAAAACTCTATTGGGCTACAGCCTTGACCTGGTGCGAGAAGGCCACAC
CACCCTTGAGGAAGTAGGCCGAATGATCCTCACCGATTCCGGATTGGAATCAGAGCGCCGCGCCAGAGCCCTTAGCACTC
TTACCTGCAACGTCTGCGGGGCAGGCCTCCAAGACGACTGGCTGGAATGTCCCTACTGTCTAACCGCTCGCCAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-148)

Type II/IV secretion system protein (T2SSE)

(215-481)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7V9D0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

53.808

94.671

0.509

Multiple sequence alignment