Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CBR65_RS11580 Genome accession   NZ_CP021382
Coordinates   2770953..2771987 (+) Length   344 a.a.
NCBI ID   WP_087466994.1    Uniprot ID   A0A1Y0FY13
Organism   Cellvibrio sp. PSBB006     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2765953..2776987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBR65_RS11555 (CBR65_11540) - 2766152..2768167 (-) 2016 WP_087466989.1 dynamin family protein -
  CBR65_RS11560 (CBR65_11545) - 2768373..2768675 (-) 303 WP_087466990.1 DUF167 family protein -
  CBR65_RS11565 (CBR65_11550) - 2768675..2769265 (-) 591 WP_087466991.1 YggT family protein -
  CBR65_RS11570 (CBR65_11555) proC 2769306..2770127 (-) 822 WP_087466992.1 pyrroline-5-carboxylate reductase -
  CBR65_RS11575 (CBR65_11560) - 2770155..2770850 (-) 696 WP_087466993.1 YggS family pyridoxal phosphate-dependent enzyme -
  CBR65_RS11580 (CBR65_11565) pilT 2770953..2771987 (+) 1035 WP_087466994.1 type IV pilus twitching motility protein PilT Machinery gene
  CBR65_RS11585 (CBR65_11570) pilU 2772102..2773247 (+) 1146 WP_087466995.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CBR65_RS11590 (CBR65_11575) ruvX 2773287..2773721 (-) 435 WP_087466996.1 Holliday junction resolvase RuvX -
  CBR65_RS11595 (CBR65_11580) - 2773771..2774361 (-) 591 WP_087466997.1 YqgE/AlgH family protein -
  CBR65_RS11600 (CBR65_11585) - 2774425..2775324 (-) 900 WP_087466998.1 energy transducer TonB -
  CBR65_RS11605 (CBR65_11590) gshB 2775321..2776289 (-) 969 WP_087469041.1 glutathione synthase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38135.88 Da        Isoelectric Point: 6.8325

>NTDB_id=230322 CBR65_RS11580 WP_087466994.1 2770953..2771987(+) (pilT) [Cellvibrio sp. PSBB006]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHSLIYEIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGNVFRDICAVPRGLVLVTGPTGSGKSTTLAAMIDYINDNKYEHVLTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPANEKSMVRSMLSESLQAVISQTLMKKNGGGRVAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGASLGMQTMDQCL
ADLVARRVISREAAKSKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=230322 CBR65_RS11580 WP_087466994.1 2770953..2771987(+) (pilT) [Cellvibrio sp. PSBB006]
ATGGATATTACCGAACTCCTCGCCTTCAGCGCCAAACAGGGCGCGTCCGACTTACACCTTTCCGCTGGCCTGCCGCCGAT
GATTCGCGTCGATGGCGACGTGCGGCGTATTAACCTGCCGCCCATGGAGCACAAGCAGGTGCACAGTTTGATCTACGAGA
TCATGAATGACAAGCAACGTAAGGACTTCGAAGAATTCCTCGAAACCGACTTCTCTTTTGAGGTGCCGGGCGTCGCCCGC
TTCCGGGTTAATGCCTTTAATCAGAACCGCGGCGCCGGCGCCGTATTCCGGACGATTCCTTCCAAGGTACTCACCATGGA
AGAGCTGGGCATGGGCAATGTGTTTCGCGATATCTGCGCCGTACCGCGCGGGTTGGTATTGGTAACCGGCCCAACCGGTT
CCGGTAAATCCACCACCCTCGCCGCTATGATTGACTACATCAACGATAACAAATACGAACACGTCCTGACGATTGAAGAC
CCGATCGAATTTGTGCATGAAAGTAAGAAGTGCCTCGTCAACCAACGGGAAGTGCACCGCGACACCCACGGCTTCGCCGA
AGCACTGCGCTCAGCATTGCGGGAAGACCCGGACATTATCCTGGTGGGTGAGATGCGTGACCTGGAAACCATTCGCCTCG
CCTTGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGTACCCTGCACACCACTTCCGCCGCAAAAACCATTGACCGTATC
GTGGATGTATTTCCGGCCAACGAAAAATCCATGGTGCGTTCAATGCTCTCGGAGTCATTGCAAGCGGTTATTTCGCAAAC
ACTGATGAAGAAAAACGGCGGCGGCCGGGTAGCAGCTCACGAAATTATGCGCGGCACCCCGGCAATTCGTAACCTGATTC
GCGAAGATAAGGTGGCCCAAATGTATTCCGCCATTCAAACCGGCGCATCGCTGGGCATGCAGACTATGGACCAATGCCTG
GCGGACCTGGTGGCGCGACGTGTCATCAGCCGTGAAGCGGCCAAGAGCAAGGCGAAGATGCCGGAGAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0FY13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

89.535

100

0.895
  pilT Pseudomonas stutzeri DSM 10701

89.244

100

0.892
  pilT Acinetobacter baumannii D1279779

83.721

100

0.837
  pilT Acinetobacter baumannii strain A118

83.721

100

0.837
  pilT Acinetobacter nosocomialis M2

83.43

100

0.834
  pilT Acinetobacter baylyi ADP1

80.814

100

0.808
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.824

98.837

0.68
  pilT Vibrio cholerae strain A1552

68.824

98.837

0.68
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment