Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CBR65_RS11585 Genome accession   NZ_CP021382
Coordinates   2772102..2773247 (+) Length   381 a.a.
NCBI ID   WP_087466995.1    Uniprot ID   A0A1Y0FYD9
Organism   Cellvibrio sp. PSBB006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2767102..2778247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBR65_RS11560 (CBR65_11545) - 2768373..2768675 (-) 303 WP_087466990.1 DUF167 family protein -
  CBR65_RS11565 (CBR65_11550) - 2768675..2769265 (-) 591 WP_087466991.1 YggT family protein -
  CBR65_RS11570 (CBR65_11555) proC 2769306..2770127 (-) 822 WP_087466992.1 pyrroline-5-carboxylate reductase -
  CBR65_RS11575 (CBR65_11560) - 2770155..2770850 (-) 696 WP_087466993.1 YggS family pyridoxal phosphate-dependent enzyme -
  CBR65_RS11580 (CBR65_11565) pilT 2770953..2771987 (+) 1035 WP_087466994.1 type IV pilus twitching motility protein PilT Machinery gene
  CBR65_RS11585 (CBR65_11570) pilU 2772102..2773247 (+) 1146 WP_087466995.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CBR65_RS11590 (CBR65_11575) ruvX 2773287..2773721 (-) 435 WP_087466996.1 Holliday junction resolvase RuvX -
  CBR65_RS11595 (CBR65_11580) - 2773771..2774361 (-) 591 WP_087466997.1 YqgE/AlgH family protein -
  CBR65_RS11600 (CBR65_11585) - 2774425..2775324 (-) 900 WP_087466998.1 energy transducer TonB -
  CBR65_RS11605 (CBR65_11590) gshB 2775321..2776289 (-) 969 WP_087469041.1 glutathione synthase -
  CBR65_RS11610 (CBR65_11595) pilG 2776608..2777003 (+) 396 WP_087466999.1 twitching motility response regulator PilG Regulator
  CBR65_RS11615 (CBR65_11600) pilH 2777070..2777432 (+) 363 WP_087467000.1 twitching motility response regulator PilH -
  CBR65_RS11620 (CBR65_11605) - 2777560..2778102 (+) 543 WP_087467001.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42514.87 Da        Isoelectric Point: 6.0942

>NTDB_id=230323 CBR65_RS11585 WP_087466995.1 2772102..2773247(+) (pilU) [Cellvibrio sp. PSBB006]
MDFDRLLALMVEKGASDLFVTAGVPPSIKLHGKIVPVTATPLAPEKVRELVLSVMNEKQRVEFLENKELNFAVSARGIGR
FRASAFYQRNLAGMVLRRIETNIPRIDDLGLPEIIKELAMTKRGLIIFVGATGTGKSTSLASMIGHRNRNSKGHIISIED
PIEFIHQHEGCIITQREVGIDTESFEIALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHRQLWMDLSLNLKAIVAQQLIPTPDGQGRRACLEVLINTPLAQDLIRKGEVSDLKELMKKSTELGMQTFDQA
LYELYDNGEITYEDALLHADSPNDLRLMIKLGSETDATYLSHAADGLSIQDDDKNDRGRMF

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=230323 CBR65_RS11585 WP_087466995.1 2772102..2773247(+) (pilU) [Cellvibrio sp. PSBB006]
ATGGATTTTGATCGTTTGTTAGCGTTGATGGTGGAAAAAGGCGCGTCGGATTTATTCGTCACCGCCGGTGTGCCGCCGTC
CATCAAGTTGCACGGCAAGATCGTTCCCGTTACCGCCACGCCACTCGCCCCCGAAAAGGTCCGCGAATTGGTGCTGAGCG
TTATGAATGAAAAACAACGTGTGGAATTCCTTGAAAATAAGGAACTGAACTTCGCCGTCAGCGCGCGCGGTATCGGCCGG
TTTCGTGCCAGCGCGTTTTACCAACGCAACCTGGCGGGCATGGTGCTGCGTCGGATTGAAACCAACATTCCGCGCATAGA
CGATCTCGGCCTGCCAGAAATTATTAAAGAACTGGCGATGACCAAGCGCGGTTTGATTATCTTTGTAGGCGCCACGGGTA
CCGGTAAATCAACATCGCTGGCATCGATGATCGGCCACCGCAACCGCAACAGTAAAGGTCACATTATTTCGATTGAAGAC
CCGATCGAATTTATTCACCAACATGAAGGCTGCATCATCACCCAACGCGAAGTGGGCATCGATACTGAATCCTTTGAAAT
TGCCCTGAAAAATACTCTGCGCCAGGCGCCGGATGTGATCCTGATTGGTGAGGTGCGGTCACGGGAAACCATGGACCACG
CCATCGCGTTTGCGGAAACCGGTCACCTGTGTTTGTGTACGCTGCACGCCAACAACGCTAACCAGGCGCTGGACCGTATC
ATTCACTTCTTCCCCGCTGACCGCCATCGCCAGTTGTGGATGGACTTGTCGCTCAACCTGAAAGCGATTGTTGCACAGCA
ATTGATCCCGACACCGGATGGACAAGGGCGACGCGCCTGTCTTGAGGTGCTGATCAATACACCGCTCGCCCAGGATCTGA
TTCGCAAAGGCGAAGTCTCGGACTTGAAAGAGCTGATGAAAAAATCTACCGAACTGGGCATGCAGACTTTCGACCAGGCA
CTCTATGAACTGTATGACAATGGTGAAATTACTTACGAGGACGCGCTGCTTCACGCTGACTCGCCCAACGACCTGCGCTT
GATGATCAAACTGGGCTCAGAAACCGATGCGACCTATTTGTCTCATGCGGCAGATGGTTTATCTATTCAGGACGATGATA
AGAATGATCGCGGGCGGATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0FYD9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

78.215

100

0.782
  pilU Acinetobacter baylyi ADP1

69.468

93.701

0.651
  pilU Vibrio cholerae strain A1552

54.571

91.864

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.058

90.551

0.399
  pilT Legionella pneumophila strain Lp02

41.493

87.927

0.365
  pilT Legionella pneumophila strain ERS1305867

41.493

87.927

0.365
  pilT Acinetobacter baumannii strain A118

40.351

89.764

0.362
  pilT Acinetobacter nosocomialis M2

40.351

89.764

0.362
  pilT Acinetobacter baumannii D1279779

40.351

89.764

0.362
  pilT Pseudomonas aeruginosa PAK

40

90.551

0.362

Multiple sequence alignment