Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CBP12_RS08200 Genome accession   NZ_CP021376
Coordinates   1777267..1778304 (-) Length   345 a.a.
NCBI ID   WP_086963996.1    Uniprot ID   -
Organism   Oceanisphaera avium strain AMac2203     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1772267..1783304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBP12_RS08180 (CBP12_08175) - 1772549..1773256 (-) 708 WP_086963992.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  CBP12_RS08185 (CBP12_08180) srmB 1773329..1774690 (+) 1362 WP_086963993.1 ATP-dependent RNA helicase SrmB -
  CBP12_RS13460 - 1774782..1774934 (+) 153 WP_157420081.1 hypothetical protein -
  CBP12_RS08190 (CBP12_08185) yaaA 1775316..1776092 (-) 777 WP_086963994.1 peroxide stress protein YaaA -
  CBP12_RS08195 (CBP12_08190) pilU 1776130..1777239 (-) 1110 WP_086963995.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CBP12_RS08200 (CBP12_08195) pilT 1777267..1778304 (-) 1038 WP_086963996.1 type IV pilus twitching motility protein PilT Machinery gene
  CBP12_RS08205 (CBP12_08200) - 1778340..1779032 (+) 693 WP_086963997.1 YggS family pyridoxal phosphate-dependent enzyme -
  CBP12_RS08210 (CBP12_08205) proC 1779060..1779881 (+) 822 WP_086965480.1 pyrroline-5-carboxylate reductase -
  CBP12_RS08215 (CBP12_08210) - 1779891..1780442 (+) 552 WP_086963998.1 YggT family protein -
  CBP12_RS08220 (CBP12_08215) - 1780427..1780741 (+) 315 WP_232455040.1 DUF167 family protein -
  CBP12_RS08225 (CBP12_08220) - 1780779..1781201 (+) 423 WP_086963999.1 DUF4426 domain-containing protein -
  CBP12_RS08230 (CBP12_08225) rdgB 1781275..1781892 (+) 618 WP_086964000.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  CBP12_RS08235 (CBP12_08230) hemW 1781892..1783037 (+) 1146 WP_086964001.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38476.89 Da        Isoelectric Point: 6.5505

>NTDB_id=230229 CBP12_RS08200 WP_086963996.1 1777267..1778304(-) (pilT) [Oceanisphaera avium strain AMac2203]
MDVTELLAFSVKHNASDLHLSAGVAPQIRVDGDIRKINLPVLEHADVHRLVYEIMNDRQRKEFEDELEVDFSFDLTGLAR
FRVNAYHQARGAAAVFRVIPSEVQTLEQLACPDIFRRIAEYQKGLVLVTGPTGSGKSTTLAAMINHINEHFNKHILTIED
PIEFVHDNKKCLVNQREVHRDTHSFSNALRSALREDPDIILVGELRDIDTIRLALTAAETGHLVFATLHTSSAAKSIDRI
VDVFPGNEKAMVRSMLSESLRAVISQTLLKQTEGGRIAAHEIMLGTPAIRNLIREDKVAQMYSVIQTGMAHGMQTLDQCL
RNLVNSGHISIVDARTQAVDSHSFQ

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=230229 CBP12_RS08200 WP_086963996.1 1777267..1778304(-) (pilT) [Oceanisphaera avium strain AMac2203]
ATGGATGTTACGGAATTATTGGCGTTTAGTGTAAAGCATAATGCCTCTGATCTGCACCTATCTGCAGGGGTAGCGCCCCA
AATTCGTGTTGATGGCGATATTAGAAAAATAAACCTGCCAGTATTAGAGCATGCCGATGTGCACCGTTTGGTTTATGAGA
TCATGAATGATCGCCAGCGCAAAGAATTTGAAGATGAGCTGGAAGTCGACTTTTCTTTTGATTTAACCGGCTTGGCGCGC
TTTCGGGTTAATGCGTATCATCAAGCCCGTGGCGCGGCCGCCGTATTTCGTGTGATCCCAAGTGAAGTACAAACCTTAGA
GCAGCTGGCTTGCCCTGATATTTTTCGCCGTATTGCTGAATATCAAAAAGGCCTAGTGTTAGTGACCGGGCCCACCGGCT
CCGGTAAGTCCACTACACTCGCCGCCATGATTAATCACATTAACGAGCACTTTAATAAGCATATTCTTACTATTGAAGAT
CCGATTGAGTTTGTGCATGACAATAAAAAATGCTTAGTTAATCAACGAGAAGTGCATCGTGATACCCACTCTTTTAGTAA
TGCGCTGCGCTCCGCTTTGCGTGAAGACCCCGATATTATTTTAGTGGGTGAGTTGCGTGATATTGATACCATTCGCTTAG
CCTTAACCGCCGCCGAAACGGGACATTTAGTGTTTGCGACGCTGCATACCTCTTCCGCCGCTAAAAGTATTGACCGCATT
GTGGATGTATTTCCGGGTAATGAAAAAGCCATGGTGCGCTCTATGCTCTCTGAGTCGCTGCGCGCTGTGATTTCACAAAC
CTTACTGAAGCAAACCGAAGGGGGGCGCATTGCCGCGCATGAAATTATGTTAGGCACTCCCGCGATCCGCAACTTGATCC
GAGAAGATAAAGTTGCGCAAATGTATTCGGTGATCCAAACCGGTATGGCTCATGGCATGCAAACACTGGATCAGTGTTTA
AGAAACTTAGTTAACTCTGGCCATATTTCTATTGTCGATGCCCGCACCCAAGCCGTAGACAGTCACAGTTTTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.328

97.101

0.722
  pilT Vibrio cholerae strain A1552

74.328

97.101

0.722
  pilT Acinetobacter nosocomialis M2

72.093

99.71

0.719
  pilT Acinetobacter baumannii D1279779

72.093

99.71

0.719
  pilT Acinetobacter baumannii strain A118

72.093

99.71

0.719
  pilT Pseudomonas aeruginosa PAK

72.093

99.71

0.719
  pilT Pseudomonas stutzeri DSM 10701

71.221

99.71

0.71
  pilT Acinetobacter baylyi ADP1

70.349

99.71

0.701
  pilT Legionella pneumophila strain Lp02

69.477

99.71

0.693
  pilT Legionella pneumophila strain ERS1305867

69.477

99.71

0.693
  pilT Neisseria meningitidis 8013

65.217

100

0.652
  pilT Neisseria gonorrhoeae MS11

64.928

100

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.504
  pilU Pseudomonas stutzeri DSM 10701

40.462

100

0.406
  pilU Vibrio cholerae strain A1552

41.493

97.101

0.403
  pilU Acinetobacter baylyi ADP1

39.412

98.551

0.388

Multiple sequence alignment