Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CBP12_RS08195 Genome accession   NZ_CP021376
Coordinates   1776130..1777239 (-) Length   369 a.a.
NCBI ID   WP_086963995.1    Uniprot ID   A0A1Y0CYZ7
Organism   Oceanisphaera avium strain AMac2203     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1771130..1782239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBP12_RS08180 (CBP12_08175) - 1772549..1773256 (-) 708 WP_086963992.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  CBP12_RS08185 (CBP12_08180) srmB 1773329..1774690 (+) 1362 WP_086963993.1 ATP-dependent RNA helicase SrmB -
  CBP12_RS13460 - 1774782..1774934 (+) 153 WP_157420081.1 hypothetical protein -
  CBP12_RS08190 (CBP12_08185) yaaA 1775316..1776092 (-) 777 WP_086963994.1 peroxide stress protein YaaA -
  CBP12_RS08195 (CBP12_08190) pilU 1776130..1777239 (-) 1110 WP_086963995.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CBP12_RS08200 (CBP12_08195) pilT 1777267..1778304 (-) 1038 WP_086963996.1 type IV pilus twitching motility protein PilT Machinery gene
  CBP12_RS08205 (CBP12_08200) - 1778340..1779032 (+) 693 WP_086963997.1 YggS family pyridoxal phosphate-dependent enzyme -
  CBP12_RS08210 (CBP12_08205) proC 1779060..1779881 (+) 822 WP_086965480.1 pyrroline-5-carboxylate reductase -
  CBP12_RS08215 (CBP12_08210) - 1779891..1780442 (+) 552 WP_086963998.1 YggT family protein -
  CBP12_RS08220 (CBP12_08215) - 1780427..1780741 (+) 315 WP_232455040.1 DUF167 family protein -
  CBP12_RS08225 (CBP12_08220) - 1780779..1781201 (+) 423 WP_086963999.1 DUF4426 domain-containing protein -
  CBP12_RS08230 (CBP12_08225) rdgB 1781275..1781892 (+) 618 WP_086964000.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40991.82 Da        Isoelectric Point: 6.7392

>NTDB_id=230228 CBP12_RS08195 WP_086963995.1 1776130..1777239(-) (pilU) [Oceanisphaera avium strain AMac2203]
MQLSPLLQAMVEQHASDLYISVGVAPTLKRQGHLVAINDTKLSARQSLALVESCMTPEGRAQFHDQKEANFAISDAQLGR
FRVSAFWQQEKAGMVIRRIQSHIPSFEELSLPDCLREVAMAKRGLVLFVGGTGAGKSTTQAAMLGYRNRHSDDHILTIED
PIEFVHQHERSIITQREVGIDTVSFAAALKSSLRQAPNVIAIGEIRSEETMNYALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHRQLQFDLSFNLKAIVAQQLIPTHEQGQRRGAFEVLLNGPLMAELIRKGELHRIKEVMSKSREQGMQTFDQA
LFDLYNQNQIGYSEALAYADSPNDVRLMIKLQAGGKGLEGGLLDNVTIE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=230228 CBP12_RS08195 WP_086963995.1 1776130..1777239(-) (pilU) [Oceanisphaera avium strain AMac2203]
ATGCAATTAAGCCCTTTGTTGCAAGCCATGGTGGAACAACACGCATCAGACTTATATATCTCAGTGGGGGTGGCCCCCAC
CCTTAAGCGCCAAGGACATTTAGTGGCGATAAACGACACTAAGCTCAGTGCAAGACAGAGTTTAGCGCTAGTAGAGTCTT
GCATGACACCGGAGGGGCGGGCGCAGTTTCATGACCAAAAAGAAGCTAACTTTGCCATTAGTGATGCCCAATTAGGCCGC
TTTAGAGTCAGTGCTTTTTGGCAACAAGAAAAAGCGGGCATGGTGATCCGGCGCATTCAAAGTCATATCCCCTCTTTTGA
AGAACTCTCTTTGCCAGATTGTTTACGAGAAGTCGCCATGGCGAAGCGTGGACTGGTGCTATTTGTGGGTGGCACTGGTG
CGGGTAAGTCGACTACTCAGGCGGCTATGCTGGGTTATCGTAATCGCCATAGTGACGATCATATTTTAACTATTGAAGAT
CCTATTGAATTTGTACACCAACATGAGCGCTCTATTATTACTCAGCGTGAGGTGGGCATAGATACCGTCTCTTTTGCTGC
CGCCTTAAAAAGCTCGCTTCGCCAAGCGCCGAACGTGATTGCGATTGGTGAGATACGCTCTGAAGAAACCATGAATTATG
CTTTGTCTTTTGCCGAAACCGGCCATTTATGTATGGCTACGTTGCACGCTAATAATGCCAACCAAGCGCTGGATCGCATT
ATGCATTTAGTCCCAGAAAGTAAGCATCGACAATTACAGTTTGATTTATCCTTTAATCTTAAAGCCATTGTGGCGCAACA
ATTAATTCCCACCCATGAACAAGGCCAGCGGCGCGGTGCTTTTGAAGTGCTACTAAATGGTCCGTTAATGGCAGAGTTGA
TCCGCAAAGGCGAGCTCCATCGCATTAAAGAAGTGATGAGCAAGTCTCGTGAGCAAGGTATGCAAACCTTTGATCAGGCC
TTGTTTGATCTTTATAACCAAAACCAAATCGGCTATAGCGAAGCGCTTGCCTATGCTGACTCTCCAAATGATGTGCGCCT
TATGATCAAGTTACAAGCAGGAGGCAAGGGCTTAGAAGGCGGCTTATTAGATAATGTAACCATTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0CYZ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

57.568

100

0.577
  pilU Pseudomonas stutzeri DSM 10701

57.423

96.748

0.556
  pilU Acinetobacter baylyi ADP1

56.534

95.393

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.282

96.206

0.388
  pilT Neisseria gonorrhoeae MS11

40.299

90.786

0.366
  pilT Acinetobacter baylyi ADP1

39.474

92.683

0.366
  pilT Legionella pneumophila strain ERS1305867

40.483

89.702

0.363
  pilT Neisseria meningitidis 8013

40.483

89.702

0.363
  pilT Legionella pneumophila strain Lp02

40.483

89.702

0.363

Multiple sequence alignment